{"id":4067,"date":"2026-10-11T06:33:31","date_gmt":"2026-10-11T06:33:31","guid":{"rendered":"https:\/\/nhdbio.com\/?page_id=4067"},"modified":"2026-10-15T03:21:40","modified_gmt":"2026-10-15T03:21:40","slug":"%d8%a7%d9%84%d8%aa%d8%b1%d9%83%d9%8a%d8%b2-%d8%a7%d9%84%d9%83%d8%aa%d9%84%d9%8a-%d9%85%d9%82%d8%a7%d8%a8%d9%84-%d8%a7%d9%84%d9%85%d9%88%d9%84%d8%a7%d8%b1%d9%8a%d8%a9","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/ar\/analytical-methods\/mass-concentration-versus-molarity\/","title":{"rendered":"mg\/mL \u0645\u0642\u0627\u0628\u0644 \u00b5M: \u0637\u0631\u064a\u0642\u062a\u0627\u0646 \u0644\u0648\u0635\u0641 \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0646\u0641\u0633\u0647"},"content":{"rendered":"<p>Is 1 mg\/mL stronger than 500 \u00b5M? You cannot tell from those two numbers alone. They describe concentration in different ways. With the peptide\u2019s molecular weight, you can put both values into the same unit.<\/p>\n<h2>\u0627\u0642\u0631\u0623 mg\/mL \u0643\u0648\u0632\u0646 \u0644\u0643\u0644 \u062d\u062c\u0645<\/h2>\n<p>\u062a\u0631\u0643\u064a\u0632 1 mg\/mL \u064a\u0639\u0646\u064a \u0623\u0646 \u0645\u0644\u0644\u064a\u0644\u062a\u0631\u064b\u0627 \u0648\u0627\u062d\u062f\u064b\u0627 \u0645\u0646 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 1 mg \u0645\u0646 \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u062a\u064a \u064a\u062c\u0631\u064a \u0639\u062f\u0651\u0647\u0627. \u0648\u0639\u0646\u062f \u0647\u0630\u0627 \u0627\u0644\u062a\u0631\u0643\u064a\u0632\u060c \u064a\u062d\u062a\u0648\u064a 2 mL \u0639\u0644\u0649 2 mg \u0648\u064a\u062d\u062a\u0648\u064a 10 mL \u0639\u0644\u0649 10 mg.<\/p>\n<p>\u0643\u0646 \u0645\u062d\u062f\u062f\u064b\u0627 \u0628\u0634\u0623\u0646 \u0627\u0644\u0645\u0627\u062f\u0629: \u0647\u0644 \u0647\u0648 \u0645\u0633\u062d\u0648\u0642 \u0645\u0648\u0632\u0648\u0646\u060c \u0623\u0645 \u0643\u0645\u064a\u0629 \u0645\u0642\u064a\u0633\u0629 \u0645\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0633\u062a\u0647\u062f\u0641\u061f<\/p>\n<h2>Read \u00b5M as molecular amount per volume<\/h2>\n<p>\u00b5M means micromolar: micromoles per litre. It is useful when comparing amounts of molecules. At the same mg\/mL, smaller molecules give more molecules per volume than larger ones.<\/p>\n<p>\u062a\u062e\u064a\u0651\u0644 \u0643\u064a\u0633\u064a\u0646 \u0645\u0646 \u0627\u0644\u062e\u0631\u0632 \u0645\u062a\u0633\u0627\u0648\u064a\u064a\u0646 \u0641\u064a \u0627\u0644\u0648\u0632\u0646. \u0627\u0644\u0643\u064a\u0633 \u0627\u0644\u0630\u064a \u064a\u062d\u062a\u0648\u064a \u062e\u0631\u0632\u064b\u0627 \u0623\u0635\u063a\u0631 \u0642\u062f \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0639\u062f\u062f \u0623\u0643\u0628\u0631 \u0645\u0646 \u0627\u0644\u062e\u0631\u0632\u0627\u062a \u0627\u0644\u0641\u0631\u062f\u064a\u0629.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t02\/en\/1.png\" alt=\"\u0627\u0644\u0634\u0643\u0644 1. \u0643\u062a\u0644\u0629 \u0627\u0644\u0645\u0633\u062d\u0648\u0642 \u0646\u0641\u0633\u0647\u0627 \u0642\u062f \u062a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0623\u0639\u062f\u0627\u062f \u0645\u062e\u062a\u0644\u0641\u0629 \u0645\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u0627\u062a.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 1. \u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u0645\u062a\u0633\u0627\u0648\u064a \u0644\u0627 \u064a\u0639\u0646\u064a \u062f\u0627\u0626\u0645\u064b\u0627 \u0623\u0639\u062f\u0627\u062f\u064b\u0627 \u0645\u062a\u0633\u0627\u0648\u064a\u0629 \u0645\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u0627\u062a.<\/figcaption><\/figure>\n<h2>Convert 1 mg\/mL into \u00b5M<\/h2>\n<p>Take an example peptide with a molecular weight of 2,000 g\/mol. First, 1 mg\/mL is the same as 1 g\/L. Divide by 2,000 to get 0.0005 mol\/L, then convert to micromolar: 500 \u00b5M.<\/p>\n<p class=\"formula\">\u00b5M = mg\/mL \u00d7 1,000,000 \u00f7 molecular weight (g\/mol)<\/p>\n<p>\u0644\u0645 \u064a\u062a\u063a\u064a\u0631 \u0634\u064a\u0621 \u0641\u064a \u0627\u0644\u0642\u0627\u0631\u0648\u0631\u0629. \u0644\u0642\u062f \u0648\u0635\u0641\u062a \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0646\u0641\u0633\u0647 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0648\u062d\u062f\u0629 \u0645\u062e\u062a\u0644\u0641\u0629.<\/p>\n<h2>\u062c\u0631\u0651\u0628 \u0648\u0632\u0646\u0627\u064b \u062c\u0632\u064a\u0626\u064a\u0627\u064b \u062b\u0627\u0646\u064a\u0627\u064b<\/h2>\n<p>At 1,000 g\/mol, the same 1 mg\/mL becomes 1,000 \u00b5M. At 2,000 g\/mol, it was 500 \u00b5M. The first solution has twice as many molecules per volume, even though the weight concentrations match.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t02\/en\/2.png\" alt=\"Figure 2. At 1 mg\/mL, hypothetical molar masses of 1,000 and 2,000 g\/mol give 1,000 and 500 \u00b5M.\"><figcaption>Figure 2. At 1 mg\/mL, the example molecular weights give 1,000 \u00b5M and 500 \u00b5M.<\/figcaption><\/figure>\n<div class=\"exercise\"><strong>\u0645\u0627 \u0647\u0648 0.2 mg\/mL \u0639\u0646\u062f \u0648\u0632\u0646 \u062c\u0632\u064a\u0626\u064a 4,000 g\/mol\u061f<\/strong><\/p>\n<details>\n<summary>\u0639\u0631\u0636 \u0627\u0644\u0625\u062c\u0627\u0628\u0629<\/summary>\n<p>0.2 \u00d7 1,000,000 \u00f7 4,000 = 50 \u00b5M.<\/p>\n<\/details>\n<\/div>\n<h2>\u062d\u0627\u0641\u0638 \u0639\u0644\u0649 \u0641\u0635\u0644 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0639\u0646 \u0627\u0644\u0643\u0645\u064a\u0629 \u0627\u0644\u0625\u062c\u0645\u0627\u0644\u064a\u0629<\/h2>\n<p>Two tubes at 250 \u00b5M can have different total amounts if one holds 1 mL and the other holds 10 mL. Concentration says how much is present per volume; total amount also needs the volume.<\/p>\n<p>\u0644\u0627 \u064a\u0645\u0643\u0646 \u0644\u0644\u062a\u062d\u0648\u064a\u0644 \u0623\u064a\u0636\u064b\u0627 \u0623\u0646 \u064a\u062e\u0628\u0631\u0643 \u0645\u0627 \u0625\u0630\u0627 \u0643\u0627\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0642\u062f \u0630\u0627\u0628 \u0628\u0627\u0644\u0643\u0627\u0645\u0644 \u0623\u0648 \u0628\u0642\u064a \u0633\u0644\u064a\u0645\u064b\u0627. \u062a\u0644\u0643 \u0641\u062d\u0648\u0635 \u0645\u0646\u0641\u0635\u0644\u0629.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t02\/en\/3.png\" alt=\"\u0627\u0644\u0634\u0643\u0644 3. \u062a\u062d\u0648\u064a\u0644 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0644\u0627 \u064a\u062b\u0628\u062a \u0645\u062d\u062a\u0648\u0649 \u0627\u0644\u0647\u062f\u0641 \u0627\u0644\u0645\u0630\u0627\u0628.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 3. \u0642\u062f \u064a\u062e\u062a\u0644\u0641 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0645\u062d\u0633\u0648\u0628 \u0639\u0646 \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0645\u0642\u0627\u0633.<\/figcaption><\/figure>\n<h2>\u0645\u0642\u0627\u0631\u0646\u0629 \u062a\u0631\u0627\u0643\u064a\u0632 KPV \u0623\u0648 LL-37<\/h2>\n<p>\u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0625\u0644\u0649 KPV \u0623\u0648 LL-37\u060c \u0633\u062c\u0651\u0644 \u0648\u062d\u062f\u0629 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0648\u0634\u0643\u0644 \u0627\u0644\u0645\u0646\u062a\u062c \u0627\u0644\u062f\u0642\u064a\u0642 \u0645\u0639\u064b\u0627. \u0625\u0630\u0627 \u0643\u0646\u062a \u062a\u0642\u0627\u0631\u0646 \u0648\u0631\u0642\u0629 \u0628\u062d\u062b\u064a\u0629 \u0628\u0633\u062c\u0644 \u0645\u0646\u062a\u062c\u060c \u0641\u062d\u0648\u0651\u0644 \u0643\u0644\u064a\u0647\u0645\u0627 \u0623\u0648\u0644\u064b\u0627 \u0625\u0644\u0649 \u0627\u0644\u0648\u062d\u062f\u0629 \u0646\u0641\u0633\u0647\u0627 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0627\u0644\u0635\u062d\u064a\u062d.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/37\/\">LL-37<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/kpv\/\">KPV<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%82%d8%aa%d8%a8%d8%a7%d8%b3\/\">\u0646\u0627\u0642\u0634 \u0645\u062a\u0637\u0644\u0628\u0627\u062a \u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0645\u0633\u062a\u0646\u062f\u0627\u062a \u0645\u0639 NHD<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d8%b7%d8%b1%d9%82-%d8%a7%d9%84%d8%aa%d8%ad%d9%84%d9%8a%d9%84%d9%8a%d8%a9\/%d9%85%d8%ad%d8%aa%d9%88%d9%89-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af-%d9%85%d9%82%d8%a7%d8%a8%d9%84-%d8%af%d8%b1%d8%ac%d8%a9-%d8%a7%d9%84%d9%86%d9%82%d8%a7%d8%a1\/\">\u062a\u0627\u0628\u0639 \u0645\u0639 \u0627\u0644\u062f\u0644\u064a\u0644 \u0627\u0644\u062a\u062d\u0644\u064a\u0644\u064a<\/a><\/p>\n<footer><strong>\u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0648\u0645\u0644\u0627\u062d\u0638\u0627\u062a \u0627\u0644\u0623\u0645\u062b\u0644\u0629<\/strong><\/p>\n<p>\u0627\u0644\u0623\u0631\u0642\u0627\u0645 \u0648\u0627\u0644\u0645\u0646\u062d\u0646\u064a\u0627\u062a \u0623\u0645\u062b\u0644\u0629 \u062a\u0639\u0644\u064a\u0645\u064a\u0629\u060c \u0648\u0644\u064a\u0633\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 \u062f\u0641\u0639\u0629 \u0645\u0646\u062a\u062c. \u062a\u0645 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0641\u064a 11 \u0623\u0643\u062a\u0648\u0628\u0631 2026.<\/p>\n<ul>\n<li><a href=\"https:\/\/www.bachem.com\/knowledge-center\/quality-control-of-amino-acids-peptides-a-guide\/\">Bachem \u00b7 peptide quality control<\/a><\/li>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/support\/calculators-and-apps\/solution-dilution-calculator\">Sigma-Aldrich \u00b7 solution dilution calculator<\/a><\/li>\n<\/ul>\n<p>\u0627\u0644\u0643\u062a\u0627\u0628\u0629 \u0628\u0645\u0633\u0627\u0639\u062f\u0629 \u0627\u0644\u0630\u0643\u0627\u0621 \u0627\u0644\u0627\u0635\u0637\u0646\u0627\u0639\u064a \u0648\u0645\u0631\u0627\u062c\u0639\u0629 \u0627\u0644\u0645\u0635\u0627\u062f\u0631. \u0645\u0648\u0627\u062f \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c \u0645\u062e\u0635\u0635\u0629 \u0644\u0644\u0628\u062d\u062b \u0648\u0627\u0644\u062a\u0637\u0648\u064a\u0631 \u0648\u0627\u0644\u062a\u0635\u0646\u064a\u0639\u060c \u0648\u0644\u064a\u0633 \u0644\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0634\u0631\u064a \u0623\u0648 \u0627\u0644\u0628\u064a\u0637\u0631\u064a.<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u062a\u0639\u0631\u0641 \u0639\u0644\u0649 \u0645\u0627 \u062a\u0642\u064a\u0633\u0647 \u0643\u0644 \u0648\u062d\u062f\u0629 \u062a\u0631\u0643\u064a\u0632\u060c \u062b\u0645 \u062d\u0648\u0650\u0651\u0644 \u0628\u064a\u0646 mg\/mL \u0648\u00b5M.<\/p>","protected":false},"author":1,"featured_media":0,"parent":865,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4067","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>mg\/mL vs \u00b5M: Two Ways to Describe the Same Peptide Solution | NHD<\/title>\n<meta name=\"description\" content=\"See 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