{"id":4070,"date":"2026-10-11T06:33:32","date_gmt":"2026-10-11T06:33:32","guid":{"rendered":"https:\/\/nhdbio.com\/?page_id=4070"},"modified":"2026-10-15T03:21:31","modified_gmt":"2026-10-15T03:21:31","slug":"%d9%85%d8%ab%d8%a7%d9%84-%d8%b9%d9%85%d9%84%d9%8a-%d8%b9%d9%84%d9%89-%d8%aa%d8%ad%d8%b6%d9%8a%d8%b1-%d9%85%d8%ad%d9%84%d9%88%d9%84-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af-%d8%a7%d9%84%d9%85%d8%ae","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/ar\/analytical-methods\/peptide-stock-preparation-worked-example\/","title":{"rendered":"\u062a\u062d\u0636\u064a\u0631 \u0645\u062d\u0644\u0648\u0644 \u0645\u062e\u0632\u0648\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f: \u062d\u0633\u0627\u0628 \u0627\u0644\u0643\u062a\u0644\u0629 \u0648\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a"},"content":{"rendered":"<p>You want 10 mL of a 100 \u00b5M peptide stock. How much material do you need? The calculation uses three numbers: concentration, final volume and molecular weight. Let us work through them before discussing the powder and solvent.<\/p>\n<h2>\u0627\u0643\u062a\u0628 \u0627\u0644\u0623\u0631\u0642\u0627\u0645 \u0627\u0644\u062b\u0644\u0627\u062b\u0629 \u0623\u0648\u0644\u0627\u064b<\/h2>\n<p>A stock solution is a relatively concentrated solution prepared for later dilution. In this example, the target is 100 \u00b5M, the final volume is 10 mL, and the molecular weight is 2,000 g\/mol.<\/p>\n<p>Convert the concentration to mol\/L and the volume to litres: 100 \u00b5M = 0.0001 mol\/L; 10 mL = 0.010 L.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t03\/en\/1.png\" alt=\"\u0627\u0644\u0634\u0643\u0644 1. \u062d\u062f\u0651\u062f \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0647\u062f\u0641 \u0648\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0642\u0628\u0644 \u0627\u062e\u062a\u064a\u0627\u0631 \u0627\u0644\u0643\u062a\u0644\u0629.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 1. \u0627\u0628\u062f\u0623 \u0628\u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0648\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0648\u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u064a.<\/figcaption><\/figure>\n<h2>\u0627\u062d\u0633\u0628 \u0627\u0644\u0643\u0645\u064a\u0629 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u0629\u060c \u062b\u0645 \u0627\u0644\u0643\u062a\u0644\u0629<\/h2>\n<p>Multiply concentration by volume: 0.0001 \u00d7 0.010 = 0.000001 mol. Multiply that amount by 2,000 g\/mol: the result is 0.002 g, or 2 mg.<\/p>\n<p>\u0644\u0630\u0644\u0643 \u064a\u062d\u062a\u0627\u062c \u0627\u0644\u0645\u062b\u0627\u0644 \u0625\u0644\u0649 2 mg \u0645\u0646 \u0635\u064a\u063a\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u062d\u062f\u062f\u0629\u060c \u0642\u0628\u0644 \u0623\u064a \u062a\u0635\u062d\u064a\u062d \u0644\u0645\u0648\u0627\u062f \u0623\u062e\u0631\u0649 \u0641\u064a \u0627\u0644\u0645\u0633\u062d\u0648\u0642.<\/p>\n<p class=\"formula\">0.0001 mol\/L \u00d7 0.010 L \u00d7 2,000 g\/mol = 0.002 g<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t03\/en\/2.png\" alt=\"Figure 2. A fictional 100 \u00b5M, 10 mL stock at 2,000 g\/mol requires 2 mg of target peptide.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 2. \u064a\u0639\u0637\u064a \u0645\u062b\u0627\u0644 \u0627\u0644\u062d\u0633\u0627\u0628 2 mg \u0645\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0633\u062a\u0647\u062f\u0641.<\/figcaption><\/figure>\n<h2>\u0645\u0627\u0630\u0627 \u0644\u0648 \u0643\u0627\u0646 \u0641\u0642\u0637 80% \u0645\u0646 \u0627\u0644\u0645\u0633\u062d\u0648\u0642 \u0647\u0648 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0633\u062a\u0647\u062f\u0641\u061f<\/h2>\n<p>Suppose a suitable quantitative test shows that the target peptide is 80% of the powder\u2019s weight. To obtain 2 mg of target peptide, the powder amount is 2 \u00f7 0.80 = 2.5 mg.<\/p>\n<p>\u064a\u062a\u0637\u0644\u0628 \u0630\u0644\u0643 \u0643\u0633\u0631\u0627\u064b \u0643\u062a\u0644\u064a\u0627\u064b \u0645\u0642\u0627\u0633\u0627\u064b \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0633\u062a\u0647\u062f\u0641. \u0644\u0627 \u064a\u0645\u0643\u0646 \u0627\u0633\u062a\u0628\u062f\u0627\u0644\u0647 \u0628\u0646\u062a\u064a\u062c\u0629 \u0646\u0642\u0627\u0621 \u0627\u0644\u0645\u0633\u0627\u062d\u0629 HPLC \u0627\u0644\u0628\u0627\u0644\u063a\u0629 98%.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t03\/en\/3.png\" alt=\"\u0627\u0644\u0634\u0643\u0644 3. \u062c\u0632\u0621 \u0645\u062d\u062a\u0648\u0649 \u0627\u0644\u0647\u062f\u0641 \u0627\u0644\u0645\u062f\u0639\u0648\u0645 \u0628\u0645\u0642\u062f\u0627\u0631 80% \u0633\u064a\u062a\u0637\u0644\u0628 2.5 mg \u0645\u0646 \u0627\u0644\u0645\u0633\u062d\u0648\u0642 \u0645\u0646 \u0623\u062c\u0644 2 mg \u0645\u0646 \u0627\u0644\u0647\u062f\u0641.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 3. \u0633\u064a\u062a\u0637\u0644\u0628 \u0627\u0644\u0643\u0633\u0631 \u0627\u0644\u0643\u062a\u0644\u064a \u0627\u0644\u0645\u0642\u0627\u0633 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0633\u062a\u0647\u062f\u0641 \u0627\u0644\u0628\u0627\u0644\u063a 80% \u0643\u0645\u064a\u0629 \u0645\u0646 \u0627\u0644\u0645\u0633\u062d\u0648\u0642 \u0645\u0642\u062f\u0627\u0631\u0647\u0627 2.5 mg.<\/figcaption><\/figure>\n<h2>\u201cMake up to 10 mL\u201d means final solution volume<\/h2>\n<p>\u0625\u0636\u0627\u0641\u0629 10 mL \u0645\u0646 \u0627\u0644\u0645\u0630\u064a\u0628 \u0648\u0625\u0643\u0645\u0627\u0644 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u062d\u062a\u0649 10 mL \u062a\u0635\u0641\u0627\u0646 \u0639\u0645\u0644\u064a\u062a\u064a\u0646 \u0645\u062e\u062a\u0644\u0641\u062a\u064a\u0646. \u064a\u0633\u062a\u062e\u062f\u0645 \u0627\u0644\u062d\u0633\u0627\u0628 \u0623\u0639\u0644\u0627\u0647 \u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0644\u0644\u0645\u062d\u0644\u0648\u0644. \u0627\u062a\u0628\u0639 \u0637\u0631\u064a\u0642\u0629 \u062a\u062d\u0636\u064a\u0631 \u0645\u0646\u0627\u0633\u0628\u0629 \u062a\u062d\u0642\u0642 \u0630\u0644\u0643 \u0627\u0644\u062d\u062c\u0645.<\/p>\n<p>\u0642\u0628\u0644 \u0627\u0644\u062a\u062d\u0636\u064a\u0631\u060c \u062a\u0623\u0643\u062f \u0645\u0646 \u0645\u0630\u064a\u0628 \u0645\u0646\u0627\u0633\u0628 \u0648\u0638\u0631\u0648\u0641 \u062a\u0639\u0627\u0645\u0644 \u0645\u0646\u0627\u0633\u0628\u0629 \u0644\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u062f\u0642\u064a\u0642\u0629. \u0647\u0630\u0647 \u0627\u0644\u062d\u0633\u0627\u0628\u0627\u062a \u0644\u0627 \u062a\u062b\u0628\u062a \u0627\u0644\u0630\u0648\u0628\u0627\u0646\u064a\u0629.<\/p>\n<h2>\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u062d\u0633\u0627\u0628 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062f\u0641\u0639\u0629 \u0623\u0635\u063a\u0631<\/h2>\n<div class=\"exercise\"><strong>With the same molecular weight, how much target peptide is needed for 5 mL at 100 \u00b5M?<\/strong><\/p>\n<details>\n<summary>\u0639\u0631\u0636 \u0627\u0644\u0625\u062c\u0627\u0628\u0629<\/summary>\n<p>\u0646\u0635\u0641 \u0627\u0644\u062d\u062c\u0645 \u064a\u062d\u062a\u0627\u062c \u0646\u0635\u0641 \u0627\u0644\u0643\u0645\u064a\u0629: 1 mg \u0645\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0647\u062f\u0641. \u0625\u0630\u0627 \u0643\u0627\u0646 \u0627\u0644\u0643\u0633\u0631 \u0627\u0644\u0643\u062a\u0644\u064a \u0627\u0644\u0645\u0642\u0627\u0633 \u0644\u0644\u0647\u062f\u0641 \u0647\u0648 80%\u060c \u0641\u0625\u0646 \u0643\u0645\u064a\u0629 \u0627\u0644\u0645\u0633\u062d\u0648\u0642 \u0633\u062a\u0643\u0648\u0646 1.25 mg.<\/p>\n<\/details>\n<\/div>\n<p>\u0633\u062c\u0651\u0644 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0645\u0648\u0632\u0648\u0646\u0629\u060c \u0648\u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u060c \u0648\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a\u060c \u0648\u0627\u0644\u0645\u0630\u064a\u0628\u060c \u0648\u0623\u064a \u062a\u0635\u062d\u064a\u062d \u0644\u0644\u0645\u062d\u062a\u0648\u0649. \u064a\u0646\u0628\u063a\u064a \u0623\u0646 \u064a\u062a\u0645\u0643\u0646 \u0634\u062e\u0635 \u0622\u062e\u0631 \u0645\u0646 \u0645\u062a\u0627\u0628\u0639\u0629 \u0627\u0644\u062d\u0633\u0627\u0628 \u062f\u0648\u0646 \u062a\u062e\u0645\u064a\u0646.<\/p>\n<h2>\u0642\u0628\u0644 \u062a\u062d\u0636\u064a\u0631 \u0645\u062d\u0644\u0648\u0644 \u0645\u062e\u0632\u0648\u0646 MOTS-c \u0623\u0648 SS-31<\/h2>\n<p>\u0644\u062a\u062e\u0637\u064a\u0637 \u0645\u062d\u0644\u0648\u0644 \u0645\u062e\u0632\u0648\u0646 MOTS-c \u0623\u0648 SS-31\u060c \u062a\u0623\u0643\u062f \u0623\u0648\u0644\u0627\u064b \u0645\u0646 \u0627\u0644\u0634\u0643\u0644 \u0627\u0644\u062f\u0642\u064a\u0642 \u0644\u0644\u0645\u0627\u062f\u0629 \u0648\u0627\u0644\u0645\u0630\u064a\u0628 \u0627\u0644\u0645\u0646\u0627\u0633\u0628. \u0627\u0633\u0623\u0644 \u0645\u0627 \u0645\u0639\u0644\u0648\u0645\u0627\u062a \u0627\u0644\u0643\u0645\u064a\u0629 \u0627\u0644\u0645\u062a\u0627\u062d\u0629 \u0644\u062a\u0644\u0643 \u0627\u0644\u0645\u0627\u062f\u0629\u061b \u0644\u0627 \u062a\u0641\u062a\u0631\u0636 \u0623\u0646 \u0645\u0644\u0635\u0642 \u0627\u0644\u0642\u0627\u0631\u0648\u0631\u0629 \u064a\u0645\u062b\u0644 \u0646\u062a\u064a\u062c\u0629 \u0645\u062d\u062a\u0648\u0649 \u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0647\u062f\u0641 \u0627\u0644\u0645\u0642\u0627\u0633.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/%d9%83%d9%84%d9%85%d8%a7%d8%aa-c-human\/\">MOTS-c<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/ss-31-%d8%a5%d9%8a%d9%84%d8%a7%d9%85%d9%8a%d8%a8%d8%b1%d9%8a%d8%aa%d9%8a%d8%af\/\">SS-31<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%82%d8%aa%d8%a8%d8%a7%d8%b3\/\">\u0646\u0627\u0642\u0634 \u0645\u062a\u0637\u0644\u0628\u0627\u062a \u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0645\u0633\u062a\u0646\u062f\u0627\u062a \u0645\u0639 NHD<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d8%b7%d8%b1%d9%82-%d8%a7%d9%84%d8%aa%d8%ad%d9%84%d9%8a%d9%84%d9%8a%d8%a9\/%d9%85%d8%ad%d8%aa%d9%88%d9%89-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af-%d9%85%d9%82%d8%a7%d8%a8%d9%84-%d8%af%d8%b1%d8%ac%d8%a9-%d8%a7%d9%84%d9%86%d9%82%d8%a7%d8%a1\/\">\u062a\u0627\u0628\u0639 \u0645\u0639 \u0627\u0644\u062f\u0644\u064a\u0644 \u0627\u0644\u062a\u062d\u0644\u064a\u0644\u064a<\/a><\/p>\n<footer><strong>\u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0648\u0645\u0644\u0627\u062d\u0638\u0627\u062a \u0627\u0644\u0623\u0645\u062b\u0644\u0629<\/strong><\/p>\n<p>\u0627\u0644\u0623\u0631\u0642\u0627\u0645 \u0648\u0627\u0644\u0645\u0646\u062d\u0646\u064a\u0627\u062a \u0623\u0645\u062b\u0644\u0629 \u062a\u0639\u0644\u064a\u0645\u064a\u0629\u060c \u0648\u0644\u064a\u0633\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 \u062f\u0641\u0639\u0629 \u0645\u0646\u062a\u062c. \u062a\u0645 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0641\u064a 11 \u0623\u0643\u062a\u0648\u0628\u0631 2026.<\/p>\n<ul>\n<li><a href=\"https:\/\/www.bachem.com\/knowledge-center\/quality-control-of-amino-acids-peptides-a-guide\/\">Bachem \u00b7 peptide quality control<\/a><\/li>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/support\/calculators-and-apps\/solution-dilution-calculator\">Sigma-Aldrich \u00b7 solution dilution calculator<\/a><\/li>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/content\/dam\/sigma-aldrich\/docs\/Sigma\/General_Information\/peptide_handling_guide.pdf\">Sigma-Aldrich \u00b7 peptide handling guide<\/a><\/li>\n<\/ul>\n<p>\u0627\u0644\u0643\u062a\u0627\u0628\u0629 \u0628\u0645\u0633\u0627\u0639\u062f\u0629 \u0627\u0644\u0630\u0643\u0627\u0621 \u0627\u0644\u0627\u0635\u0637\u0646\u0627\u0639\u064a \u0648\u0645\u0631\u0627\u062c\u0639\u0629 \u0627\u0644\u0645\u0635\u0627\u062f\u0631. \u0645\u0648\u0627\u062f \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c \u0645\u062e\u0635\u0635\u0629 \u0644\u0644\u0628\u062d\u062b \u0648\u0627\u0644\u062a\u0637\u0648\u064a\u0631 \u0648\u0627\u0644\u062a\u0635\u0646\u064a\u0639\u060c \u0648\u0644\u064a\u0633 \u0644\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0634\u0631\u064a \u0623\u0648 \u0627\u0644\u0628\u064a\u0637\u0631\u064a.<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u0627\u062d\u0633\u0628 \u0627\u0644\u0643\u0645\u064a\u0629 \u0627\u0644\u062a\u064a \u062a\u062d\u062a\u0627\u062c\u0647\u0627 \u0644\u062a\u062d\u0636\u064a\u0631 \u0645\u062d\u0644\u0648\u0644 \u0645\u062e\u0632\u0648\u0646\u060c \u0648\u0627\u0641\u0647\u0645 \u0633\u0628\u0628 \u0623\u0647\u0645\u064a\u0629 \u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a.<\/p>","protected":false},"author":1,"featured_media":0,"parent":865,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4070","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Preparing a Peptide Stock: Calculate Mass and Final Volume | NHD<\/title>\n<meta name=\"description\" content=\"Calculate the amount you need for a stock solution, and understand why final volume matters.\" \/>\n<meta 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