{"id":2695,"date":"2026-09-26T04:34:36","date_gmt":"2026-09-26T04:34:36","guid":{"rendered":"https:\/\/nhdbio.com\/temporin-a-counterion-selectivity-136-022-060-study\/"},"modified":"2026-10-01T14:29:48","modified_gmt":"2026-10-01T14:29:48","slug":"%d8%af%d8%b1%d8%a7%d8%b3%d8%a9-%d8%a7%d9%86%d8%aa%d9%82%d8%a7%d8%a6%d9%8a%d8%a9-%d8%a7%d9%84%d8%a3%d9%8a%d9%88%d9%86-%d8%a7%d9%84%d9%85%d9%82%d8%a7%d8%a8%d9%84-%d9%81%d9%8a-temporin-a-136-022-060","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ar\/temporin-a-counterion-selectivity-136-022-060-study\/","title":{"rendered":"\u0623\u0645\u0644\u0627\u062d Temporin A: \u0644\u0645\u0627\u0630\u0627 \u064a\u062a\u063a\u064a\u0631 \u0645\u0624\u0634\u0631 \u0627\u0644\u0627\u0646\u062a\u0642\u0627\u0626\u064a\u0629 \u0628\u0627\u062e\u062a\u0644\u0627\u0641 \u0627\u0644\u0623\u064a\u0648\u0646 \u0627\u0644\u0645\u0642\u0627\u0628\u0644\u061f"},"content":{"rendered":"<p>An in-vitro antimicrobial study shows why the salt form belongs in the material identity.<\/p>\n<h2>\u0645\u0627 \u0627\u062e\u062a\u0628\u0631\u062a\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0641\u0639\u0644\u064a\u064b\u0627<\/h2>\n<p>Researchers prepared acetate, chloride, and trifluoroacetate salts of five antimicrobial peptides and measured activity against Staphylococcus aureus alongside red-cell hemolysis and cell-based toxicity. For temporin A, Table 6 reports a selectivity index of 1.34 for the TFA salt, 0.22 for acetate, and 0.60 for chloride. Other tested peptides did not follow one universal ordering of salt performance.<\/p>\n<h2>\u0623\u0631\u0642\u0627\u0645 \u062a\u0633\u062a\u062d\u0642 \u0627\u0644\u0642\u0631\u0627\u0621\u0629 \u0641\u064a \u0633\u064a\u0627\u0642\u0647\u0627<\/h2>\n<table>\n<thead>\n<tr>\n<th>\u0645\u0642\u064a\u0627\u0633 \u0627\u0644\u062f\u0631\u0627\u0633\u0629<\/th>\n<th>\u0627\u0644\u0646\u062a\u064a\u062c\u0629 \u0627\u0644\u0645\u0646\u0634\u0648\u0631\u0629<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Temporin A TFA<\/td>\n<td>1.34 (Table 6)<\/td>\n<\/tr>\n<tr>\n<td>Temporin A acetate<\/td>\n<td>0.22<\/td>\n<\/tr>\n<tr>\n<td>Temporin A chloride<\/td>\n<td>0.60<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>The selectivity index is a ratio from the paper\u2019s assays, not a clinical efficacy or safety score. The highest numerical value in this comparison does not make TFA a universally preferred salt: the same study described peptide-dependent differences and multiple assay endpoints. The tested lots, organisms, and cell systems define the evidence boundary.<\/p>\n<h2>Why the paper also contains 1.36<\/h2>\n<p>The discussion gives 1.36 for temporin A trifluoroacetate, whereas Table 6 gives 1.34. The tabulated IC50 of 7.91 \u00b5g\/mL divided by the mean MIC of 5.9 \u00b5g\/mL is approximately 1.34. We use the table value and flag the disagreement instead of treating both figures as interchangeable. This correction does not resolve uncertainties in the biological assays or establish a clinical safety margin.<\/p>\n<h2>\u0645\u0627 \u064a\u062c\u0628 \u0625\u062f\u0631\u0627\u062c\u0647 \u0641\u064a \u0637\u0644\u0628 \u0627\u0644\u0639\u0631\u0636 \u0623\u0648 \u062e\u0637\u0629 \u0627\u0644\u062a\u062c\u0631\u0628\u0629<\/h2>\n<p>When reproducing an antimicrobial-peptide experiment, specify the peptide sequence and counterion together. Record whether concentrations are expressed as total salt mass or peptide content, and ask for a measured counterion composition rather than a name inferred from purification solvent. Compare biological results only after aligning the material and assay conditions.<\/p>\n<h2>\u062d\u062f \u0627\u0644\u0623\u062f\u0644\u0629<\/h2>\n<p>This article summarizes external in-vitro research. It makes no treatment recommendation, claims no human outcome, and does not report an NHD lot result.<\/p>\n<h2>\u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0648\u0627\u0644\u062e\u0637\u0648\u0629 \u0627\u0644\u062a\u0627\u0644\u064a\u0629<\/h2>\n<p>\u0627\u0644\u0628\u062d\u062b \u0627\u0644\u0623\u0633\u0627\u0633\u064a: <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC5917001\/\">\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0623\u064a\u0648\u0646 \u0627\u0644\u0645\u0642\u0627\u0628\u0644 \u0639\u0644\u0649 \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u0645\u0636\u0627\u062f \u0644\u0644\u0645\u0643\u0648\u0631\u0627\u062a \u0627\u0644\u0639\u0646\u0642\u0648\u062f\u064a\u0629 \u0648\u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0644\u062f\u0649 \u0628\u0628\u062a\u064a\u062f\u0627\u062a \u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a \u0645\u062e\u062a\u0627\u0631\u0629<\/a>. \u0647\u0630\u0647 \u0627\u0644\u0623\u0631\u0642\u0627\u0645 \u0627\u0644\u062e\u0627\u0631\u062c\u064a\u0629 \u0644\u064a\u0633\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 \u0644\u062f\u0641\u0639\u0629 NHD.<\/p>\n<p>\u062f\u0644\u064a\u0644 NHD \u0630\u0648 \u0627\u0644\u0635\u0644\u0629: <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af%d8%8c-%d8%a7%d9%84%d8%a3%d9%8a%d9%88%d9%86%d8%a7%d8%aa-%d8%a7%d9%84%d9%85%d8%b6%d8%a7%d8%af%d8%a9%d8%8c-%d8%a7%d9%84%d9%85%d8%a7%d8%a1%d8%8c-%d8%a7%d9%84\/\">\u0627\u0642\u0631\u0623 \u0627\u0644\u0645\u0642\u0627\u0644 \u0630\u0627 \u0627\u0644\u0635\u0644\u0629<\/a>. \u0644\u0633\u0624\u0627\u0644 \u062e\u0627\u0635 \u0628\u0645\u0627\u062f\u0629 \u0645\u0639\u064a\u0646\u0629\u060c <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%82%d8%aa%d8%a8%d8%a7%d8%b3\/\">\u0627\u0637\u0644\u0628 \u0639\u0631\u0636 \u0633\u0639\u0631 \u0645\u0643\u062a\u0648\u0628<\/a> \u0645\u0639 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0648\u0627\u0644\u0643\u0645\u064a\u0629 \u0648\u0627\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0645\u0642\u0635\u0648\u062f \u0648\u0627\u0644\u0633\u062c\u0644\u0627\u062a \u0627\u0644\u0645\u0637\u0644\u0648\u0628\u0629.<\/p>","protected":false},"excerpt":{"rendered":"<p>An in-vitro antimicrobial study shows why the salt form belongs in the material identity.<\/p>","protected":false},"author":1,"featured_media":2742,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"","pim_article_type":"Primary-literature evidence note","pim_evidence_level":"External laboratory or process evidence; not clinical or NHD lot validation","pim_reviewer":"AI-assisted source cross-check (not independent expert peer review)","pim_reviewed_date":"2026-09-28","pim_editorial_note":"This article summarizes external in-vitro research. It makes no treatment recommendation, claims no human outcome, and does not report an NHD lot result.","pim_review_status":"reviewed","pim_review_reason":"","pim_creation_method":"AI-assisted writing and source verification. Numerical claims were checked against the linked full-text records, with source inconsistencies identified in the article. This is not independent scientific peer review or verification of a supplied lot.","pim_sources":"[{\"title\":\"Counter-ion effect on antistaphylococcal activity and cytotoxicity of selected antimicrobial peptides\",\"url\":\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC5917001\/\",\"note\":\"\"}]","footnotes":""},"categories":[19],"tags":[],"class_list":["post-2695","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-published-study-cases"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Temporin A Salt Forms: Why Selectivity Index Changed Across Counterions - NHD<\/title>\n<meta name=\"description\" content=\"An in-vitro antimicrobial study shows why the salt form belongs in the material identity. 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