{"id":3026,"date":"2026-10-03T16:08:47","date_gmt":"2026-10-03T16:08:47","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3026"},"modified":"2026-10-15T03:53:29","modified_gmt":"2026-10-15T03:53:29","slug":"%d8%ad%d8%af%d9%88%d8%af-%d8%a7%d9%84%d9%83%d8%b4%d9%81-%d9%88%d8%a7%d9%84%d8%aa%d9%82%d8%af%d9%8a%d8%b1-%d8%a7%d9%84%d9%83%d9%85%d9%8a-%d9%84%d8%b4%d9%87%d8%a7%d8%af%d8%a9-%d8%aa%d8%ad%d9%84%d9%8a","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ar\/peptide-coa-not-detected-detection-quantitation-limits\/","title":{"rendered":"\u201c\u063a\u064a\u0631 \u0645\u0643\u062a\u0634\u064e\u0641\u201d \u0644\u064a\u0633 \u0635\u0641\u0631\u064b\u0627: \u0627\u0642\u0631\u0623 \u062d\u062f\u0648\u062f \u0627\u0644\u0643\u0634\u0641 \u0648\u0627\u0644\u062a\u0642\u062f\u064a\u0631 \u0627\u0644\u0643\u0645\u064a"},"content":{"rendered":"<p>A report says \u201cnot detected.\u201d Can you write that the sample contains none of that substance? Not quite. Every method has a limit to what it can see and measure. The report needs to tell you that limit.<\/p>\n<h2>\u0631\u0624\u064a\u0629 \u0634\u064a\u0621 \u0645\u0627 \u0648\u0642\u064a\u0627\u0633\u0647 \u0628\u062f\u0642\u0629 \u0623\u0645\u0631\u0627\u0646 \u0645\u062e\u062a\u0644\u0641\u0627\u0646<\/h2>\n<p>\u064a\u062a\u0639\u0644\u0642 \u062d\u062f\u0651 \u0627\u0644\u0643\u0634\u0641 \u0628\u0645\u0627 \u0625\u0630\u0627 \u0643\u0627\u0646 \u064a\u0645\u0643\u0646 \u0627\u0644\u062a\u0639\u0631\u0641 \u0639\u0644\u0649 \u0645\u0627\u062f\u0629 \u0645\u0646\u062e\u0641\u0636\u0629 \u0627\u0644\u0645\u0633\u062a\u0648\u0649 \u0628\u0627\u0644\u0637\u0631\u064a\u0642\u0629. \u0648\u064a\u062a\u0639\u0644\u0642 \u062d\u062f\u0651 \u0627\u0644\u062a\u0642\u062f\u064a\u0631 \u0627\u0644\u0643\u0645\u064a \u0628\u0645\u0627 \u0625\u0630\u0627 \u0643\u0627\u0646 \u064a\u0645\u0643\u0646 \u0642\u064a\u0627\u0633 \u0643\u0645\u064a\u062a\u0647\u0627 \u0628\u0623\u062f\u0627\u0621 \u0645\u0646\u0627\u0633\u0628. \u0642\u062f \u062a\u0643\u0648\u0646 \u0627\u0644\u0625\u0634\u0627\u0631\u0629 \u0627\u0644\u062e\u0627\u0641\u062a\u0629 \u0642\u0627\u0628\u0644\u0629 \u0644\u0644\u062a\u0639\u0631\u0641 \u0642\u0628\u0644 \u0623\u0646 \u062a\u0643\u0648\u0646 \u0643\u0645\u064a\u062a\u0647\u0627 \u0645\u0648\u062b\u0648\u0642\u0629.<\/p>\n<p>\u0642\u062f \u064a\u064f\u0639\u0631\u064e\u0651\u0641 \u062d\u062f\u0651 \u0627\u0644\u0625\u0628\u0644\u0627\u063a \u0628\u0634\u0643\u0644 \u0645\u0646\u0641\u0635\u0644 \u0641\u064a \u0627\u0644\u0625\u062c\u0631\u0627\u0621. \u0627\u0633\u0623\u0644 \u0639\u0646 \u0627\u0644\u062d\u062f\u0651 \u0627\u0644\u0630\u064a \u064a\u0633\u062a\u062e\u062f\u0645\u0647 \u0627\u0644\u062a\u0642\u0631\u064a\u0631 \u0641\u0639\u0644\u0627\u064b.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t17\/en\/1.png\" alt=\"Figure 1. Detection and quantitation are different questions.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 1. \u0627\u0644\u0643\u0634\u0641 \u0648\u0642\u064a\u0627\u0633 \u0627\u0644\u0643\u0645\u064a\u0629 \u0627\u0644\u0645\u0648\u062b\u0648\u0642 \u064a\u062c\u064a\u0628\u0627\u0646 \u0639\u0646 \u0633\u0624\u0627\u0644\u064a\u0646 \u0645\u062e\u062a\u0644\u0641\u064a\u0646.<\/figcaption><\/figure>\n<h2>\u0627\u062a\u0628\u0639 \u0645\u062b\u0627\u0644\u0627\u064b \u0648\u0627\u062d\u062f\u064b\u0627 \u0645\u0646 \u0627\u0644\u0623\u062f\u0646\u0649 \u0625\u0644\u0649 \u0627\u0644\u0623\u0639\u0644\u0649<\/h2>\n<p>In this teaching example, the detection limit is about 0.2 \u00b5g\/g and the quantitation limit is 0.6 \u00b5g\/g. Below the detection capability, presence cannot be reliably established by these rules.<\/p>\n<p>\u0641\u0648\u0642 \u062d\u062f\u0651 \u0627\u0644\u0643\u0634\u0641 \u0648\u0644\u0643\u0646 \u062f\u0648\u0646 0.6\u060c \u0642\u062f \u064a\u0643\u0648\u0646 \u0647\u0646\u0627\u0643 \u062f\u0644\u064a\u0644 \u0639\u0644\u0649 \u0627\u0644\u0648\u062c\u0648\u062f \u062f\u0648\u0646 \u0643\u0645\u064a\u0629 \u0631\u0642\u0645\u064a\u0629 \u0645\u0648\u062b\u0648\u0642\u0629. \u0641\u064a \u0627\u0644\u0646\u0637\u0627\u0642 \u0627\u0644\u0643\u0645\u064a \u0627\u0644\u0645\u0639\u062a\u0645\u062f\u060c \u064a\u0645\u0643\u0646 \u0644\u0644\u0637\u0631\u064a\u0642\u0629 \u0623\u0646 \u062a\u062f\u0639\u0645 \u0646\u062a\u064a\u062c\u0629 \u0643\u0645\u064a\u0629.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t17\/en\/2.png\" alt=\"Figure 2. Illustrative thresholds: below detection, detected below quantitation, and within a supported quantitative range.\"><figcaption>Figure 2. Example detection and quantitation limits of 0.2 and 0.6 \u00b5g\/g.<\/figcaption><\/figure>\n<h2>\u0647\u0644 \u064a\u0644\u0628\u064a \u0647\u0630\u0627 \u0627\u0644\u062d\u062f \u0645\u062a\u0637\u0644\u0628\u0627\u062a\u0643\u061f<\/h2>\n<p>Suppose you require less than 0.1 \u00b5g\/g, but the result only supports less than 0.2 \u00b5g\/g. It does not settle whether the sample meets the stricter requirement.<\/p>\n<p>\u0642\u062f \u064a\u0628\u062f\u0648 \u0627\u0644\u0631\u0642\u0645\u0627\u0646 \u0635\u063a\u064a\u0631\u064a\u0646. \u0644\u0643\u0646 \u0627\u0644\u0641\u0631\u0642 \u0628\u064a\u0646\u0647\u0645\u0627 \u0644\u0627 \u064a\u0632\u0627\u0644 \u0645\u0647\u0645\u064b\u0627. \u0642\u0627\u0631\u0646 \u0627\u0644\u0645\u0627\u062f\u0629 \u0646\u0641\u0633\u0647\u0627 \u0648\u0627\u0644\u0648\u062d\u062f\u0627\u062a \u0648\u0623\u0633\u0627\u0633 \u0627\u0644\u0639\u064a\u0646\u0629 \u0646\u0641\u0633\u0647.<\/p>\n<h2>A blank box is not \u201cnot detected\u201d<\/h2>\n<p>A blank field may mean no result was supplied. \u201cNot tested\u201d means the test was not performed. \u201cNot detected\u201d describes an outcome under a method\u2019s reporting rules. None automatically means an exact zero.<\/p>\n<p>\u0642\u062f \u062a\u0643\u0648\u0646 \u0642\u064a\u0645\u0629 0.000 \u0627\u0644\u0645\u0639\u0631\u0648\u0636\u0629 \u0645\u0642\u0631\u064e\u0651\u0628\u0629 \u0623\u0648 \u0645\u0639\u0627\u0644\u064e\u062c\u0629 \u0623\u064a\u0636\u064b\u0627. \u0627\u0633\u0623\u0644 \u0643\u064a\u0641 \u062c\u0631\u0649 \u062a\u0648\u0644\u064a\u062f\u0647\u0627 \u0642\u0628\u0644 \u0627\u0644\u062a\u0639\u0627\u0645\u0644 \u0645\u0639\u0647\u0627 \u0643\u0642\u064a\u0645\u0629 \u062f\u0642\u064a\u0642\u0629.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t17\/en\/3.png\" alt=\"Figure 3. Report ND with the method, unit and reporting threshold.\"><figcaption>Figure 3. Read \u201cnot detected\u201d together with the method, unit and reporting limit.<\/figcaption><\/figure>\n<h2>\u0627\u0637\u0631\u062d \u0633\u0624\u0627\u0644\u0627\u064b \u0648\u0627\u062d\u062f\u0627\u064b \u0643\u0627\u0645\u0644\u0627\u064b<\/h2>\n<p>\u0627\u0633\u0623\u0644: \u0645\u0627 \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u062a\u064a \u062a\u0645 \u0627\u062e\u062a\u0628\u0627\u0631\u0647\u0627\u060c \u0648\u0628\u0623\u064a \u0637\u0631\u064a\u0642\u0629\u060c \u0648\u0628\u0623\u064a \u0648\u062d\u062f\u0627\u062a\u060c \u0648\u0628\u0623\u064a \u062d\u062f \u0625\u0628\u0644\u0627\u063a\u061f \u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u0630\u064a\u0641\u0627\u0646 \u0627\u0644\u062f\u0627\u062e\u0644\u064a \u0648\u0627\u0644\u0645\u0630\u064a\u0628 \u0627\u0644\u0645\u062a\u0628\u0642\u064a\u060c \u0639\u0644\u0649 \u0633\u0628\u064a\u0644 \u0627\u0644\u0645\u062b\u0627\u0644\u060c \u062a\u0641\u062d\u0635 \u0645\u0648\u0627\u062f \u0645\u062e\u062a\u0644\u0641\u0629.<\/p>\n<div class=\"exercise\"><strong>A report states less than 1 \u00b5g\/g. Does that establish less than 0.5 \u00b5g\/g?<\/strong><\/p>\n<details>\n<summary>\u0639\u0631\u0636 \u0627\u0644\u0625\u062c\u0627\u0628\u0629<\/summary>\n<p>No. The range from 0.5 to 1 \u00b5g\/g has not been excluded.<\/p>\n<\/details>\n<\/div>\n<h2>\u0637\u0644\u0628 \u0646\u062a\u0627\u0626\u062c \u0644\u0640 LL-37 \u0623\u0648 GHK-Cu<\/h2>\n<p>\u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0644\u0640 LL-37 \u0623\u0648 GHK-Cu\u060c \u062d\u062f\u062f \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u062a\u064a \u062a\u0631\u064a\u062f \u0642\u064a\u0627\u0633\u0647\u0627 \u0648\u0642\u062f\u0631\u0629 \u0627\u0644\u0625\u0628\u0644\u0627\u063a \u0627\u0644\u062a\u064a \u062a\u062d\u062a\u0627\u062c\u0647\u0627. \u062a\u0623\u0643\u062f \u0645\u0646 \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u062a\u064a \u0623\u064f\u062c\u0631\u064a\u062a \u0641\u0639\u0644\u0627\u064b. \u0646\u0642\u0627\u0621 HPLC \u0644\u0627 \u064a\u0648\u0641\u0631 \u0646\u062a\u064a\u062c\u0629 \u0627\u0644\u0630\u064a\u0641\u0627\u0646 \u0627\u0644\u062f\u0627\u062e\u0644\u064a.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/37\/\">LL-37<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/ghk-cu\/\">GHK-Cu<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%82%d8%aa%d8%a8%d8%a7%d8%b3\/\">\u0646\u0627\u0642\u0634 \u0645\u062a\u0637\u0644\u0628\u0627\u062a \u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0645\u0633\u062a\u0646\u062f\u0627\u062a \u0645\u0639 NHD<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d8%b7%d8%b1%d9%82-%d8%a7%d9%84%d8%aa%d8%ad%d9%84%d9%8a%d9%84%d9%8a%d8%a9\/%d9%83%d9%8a%d9%81%d9%8a%d8%a9-%d9%82%d8%b1%d8%a7%d8%a1%d8%a9-%d8%b4%d9%87%d8%a7%d8%af%d8%a9-%d8%aa%d8%ad%d9%84%d9%8a%d9%84-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af\/\">\u062a\u0627\u0628\u0639 \u0645\u0639 \u0627\u0644\u062f\u0644\u064a\u0644 \u0627\u0644\u062a\u062d\u0644\u064a\u0644\u064a<\/a><\/p>\n<footer><strong>\u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0648\u0645\u0644\u0627\u062d\u0638\u0627\u062a \u0627\u0644\u0623\u0645\u062b\u0644\u0629<\/strong><\/p>\n<p>\u0627\u0644\u0623\u0631\u0642\u0627\u0645 \u0648\u0627\u0644\u0645\u0646\u062d\u0646\u064a\u0627\u062a \u0623\u0645\u062b\u0644\u0629 \u062a\u0639\u0644\u064a\u0645\u064a\u0629\u060c \u0648\u0644\u064a\u0633\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 \u062f\u0641\u0639\u0629 \u0645\u0646\u062a\u062c. \u062a\u0645 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0641\u064a 11 \u0623\u0643\u062a\u0648\u0628\u0631 2026.<\/p>\n<ul>\n<li><a href=\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\">ICH Q2(R2) \u00b7 analytical validation<\/a><\/li>\n<\/ul>\n<p>\u0627\u0644\u0643\u062a\u0627\u0628\u0629 \u0628\u0645\u0633\u0627\u0639\u062f\u0629 \u0627\u0644\u0630\u0643\u0627\u0621 \u0627\u0644\u0627\u0635\u0637\u0646\u0627\u0639\u064a \u0648\u0645\u0631\u0627\u062c\u0639\u0629 \u0627\u0644\u0645\u0635\u0627\u062f\u0631. \u0645\u0648\u0627\u062f \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c \u0645\u062e\u0635\u0635\u0629 \u0644\u0644\u0628\u062d\u062b \u0648\u0627\u0644\u062a\u0637\u0648\u064a\u0631 \u0648\u0627\u0644\u062a\u0635\u0646\u064a\u0639\u060c \u0648\u0644\u064a\u0633 \u0644\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0634\u0631\u064a \u0623\u0648 \u0627\u0644\u0628\u064a\u0637\u0631\u064a.<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u062a\u0639\u0631\u0651\u0641 \u0639\u0644\u0649 \u0645\u0627 \u062a\u062e\u0628\u0631\u0643 \u0628\u0647 \u0639\u0628\u0627\u0631\u0629 \u201c\u063a\u064a\u0631 \u0645\u064f\u0643\u062a\u0634\u064e\u0641\u201d\u060c \u0648\u0645\u0627 \u0627\u0644\u0631\u0642\u0645 \u0627\u0644\u0630\u064a \u0644\u0627 \u062a\u0632\u0627\u0644 \u0628\u062d\u0627\u062c\u0629 \u0625\u0644\u064a\u0647.<\/p>","protected":false},"author":1,"featured_media":3054,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"318,344","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and clearly labeled examples","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Reference data and teaching examples are separated from NHD lot results; product specifications and availability require confirmation.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"AI-assisted drafting, source checks, translation and generated comic illustration. This is not independent peer review or supplied-lot verification.","pim_sources":"[{\"title\":\"ICH Q2(R2): Validation of Analytical Procedures\",\"note\":\"International Council for Harmonisation. Step 4, adopted November 2023; error-corrected 2025.\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2%28R2%29_Guideline_2023_1130_ErrorCorrection_2025.pdf\"},{\"title\":\"Teaching reference \u2014 database.ich.org \u2014 ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\",\"note\":\"\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\"},{\"title\":\"ICH Q2(R2) Validation of Analytical Procedures\",\"note\":\"ICH Q2(R2) Validation of Analytical Procedures\",\"url\":\"https:\/\/www.ema.europa.eu\/en\/scientific-guidelines\/ich-q2r2-validation-analytical-procedures\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3026","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u201cNot Detected\u201d Is Not Zero: Read Detection and Quantitation Limits | NHD<\/title>\n<meta name=\"description\" content=\"Learn what \u201cnot detected\u201d tells you, and what number you still need.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ar\/\u062d\u062f\u0648\u062f-\u0627\u0644\u0643\u0634\u0641-\u0648\u0627\u0644\u062a\u0642\u062f\u064a\u0631-\u0627\u0644\u0643\u0645\u064a-\u0644\u0634\u0647\u0627\u062f\u0629-\u062a\u062d\u0644\u064a\/\" \/>\n<meta property=\"og:locale\" content=\"ar_AR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u201cNot Detected\u201d Is Not Zero: Read Detection and Quantitation Limits\" \/>\n<meta property=\"og:description\" content=\"Learn what \u201cnot detected\u201d tells you, and what number you still need.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nhdbio.com\/ar\/\u062d\u062f\u0648\u062f-\u0627\u0644\u0643\u0634\u0641-\u0648\u0627\u0644\u062a\u0642\u062f\u064a\u0631-\u0627\u0644\u0643\u0645\u064a-\u0644\u0634\u0647\u0627\u062f\u0629-\u062a\u062d\u0644\u064a\/\" \/>\n<meta property=\"og:site_name\" content=\"NHD\" \/>\n<meta property=\"article:published_time\" content=\"2026-10-03T16:08:47+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-10-15T03:53:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/limits-cover-1024x683.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1024\" \/>\n\t<meta property=\"og:image:height\" content=\"683\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"NHD Technical Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"twitter:label1\" content=\"\u0643\u064f\u062a\u0628 \u0628\u0648\u0627\u0633\u0637\u0629\" \/>\n\t<meta name=\"twitter:data1\" content=\"NHD Technical Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u0648\u0642\u062a \u0627\u0644\u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0645\u064f\u0642\u062f\u0651\u0631\" \/>\n\t<meta name=\"twitter:data2\" content=\"\u062f\u0642\u064a\u0642\u062a\u0627\u0646\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/\"},\"author\":{\"name\":\"NHD Technical Team\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/person\\\/0ffb0dec7fda0f720073fc468fac6fc1\"},\"headline\":\"\u201cNot Detected\u201d Is Not Zero: Read Detection and Quantitation Limits\",\"datePublished\":\"2026-10-03T16:08:47+00:00\",\"dateModified\":\"2026-10-15T03:53:29+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/\"},\"wordCount\":469,\"publisher\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/limits-cover.png\",\"articleSection\":[\"Evidence &amp; 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