{"id":3027,"date":"2026-10-03T16:09:26","date_gmt":"2026-10-03T16:09:26","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3027"},"modified":"2026-10-11T07:38:55","modified_gmt":"2026-10-11T07:38:55","slug":"%d9%86%d8%b3%d8%a8%d8%a9-dmso-%d8%a7%d9%84%d9%86%d9%87%d8%a7%d8%a6%d9%8a%d8%a9-%d9%84%d8%aa%d8%ae%d9%81%d9%8a%d9%81-%d9%85%d8%ad%d9%84%d9%88%d9%84-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af-%d8%a7","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ar\/peptide-stock-dilution-final-dmso-percentage\/","title":{"rendered":"\u0647\u0644 \u062a\u0642\u0648\u0645 \u0628\u062a\u062e\u0641\u064a\u0641 \u0645\u062d\u0644\u0648\u0644 \u0645\u062e\u0632\u0648\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f\u061f \u0627\u062d\u0633\u0628 \u0627\u0644\u0646\u0633\u0628\u0629 \u0627\u0644\u0645\u0626\u0648\u064a\u0629 \u0627\u0644\u0646\u0647\u0627\u0626\u064a\u0629 \u0644\u0640 DMSO"},"content":{"rendered":"<p>\u0625\u0636\u0627\u0641\u0629 \u0635\u063a\u064a\u0631\u0629 \u0645\u0646 \u0645\u062d\u0644\u0648\u0644 \u0645\u062e\u0632\u0648\u0646 \u0628\u0628\u062a\u064a\u062f \u0645\u0631\u0643\u0651\u0632 \u062a\u0636\u064a\u0641 \u0623\u064a\u0636\u064b\u0627 \u0645\u0630\u064a\u0628\u0647. \u0625\u0630\u0627 \u0643\u0627\u0646 \u0627\u0644\u0645\u062e\u0632\u0648\u0646 \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 DMSO\u060c \u0641\u0623\u0646\u062a \u0628\u062d\u0627\u062c\u0629 \u0625\u0644\u0649 \u062a\u062a\u0628\u0639 \u0630\u0644\u0643 \u0627\u0644\u0633\u0627\u0626\u0644 \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u0627\u0644\u0628\u0628\u062a\u064a\u062f. \u0627\u0644\u062d\u0633\u0627\u0628\u0627\u0646 \u064a\u062c\u064a\u0628\u0627\u0646 \u0639\u0646 \u0633\u0624\u0627\u0644\u064a\u0646 \u0645\u062e\u062a\u0644\u0641\u064a\u0646.<\/p>\n<h2>\u0627\u062d\u0633\u0628 \u062d\u062c\u0645 \u0627\u0644\u0645\u0630\u064a\u0628 \u0623\u0648\u0644\u0627\u064b<\/h2>\n<p>DMSO \u0647\u0648 \u0645\u0630\u064a\u0628 \u064a\u064f\u0633\u062a\u062e\u062f\u0645 \u0623\u062d\u064a\u0627\u0646\u064b\u0627 \u0641\u064a \u0627\u0644\u0645\u062d\u0627\u0644\u064a\u0644 \u0627\u0644\u0645\u062e\u0632\u0648\u0646\u0629 \u0627\u0644\u0628\u062d\u062b\u064a\u0629. \u0644\u062d\u0633\u0627\u0628 \u0627\u0644\u0646\u0633\u0628\u0629 \u0627\u0644\u0645\u0626\u0648\u064a\u0629 \u0644\u062d\u062c\u0645\u0647 \u0627\u0644\u0646\u0647\u0627\u0626\u064a\u060c \u0623\u0648\u062c\u062f \u0639\u062f\u062f \u0627\u0644\u0645\u064a\u0643\u0631\u0648\u0644\u062a\u0631\u0627\u062a \u0627\u0644\u0645\u0636\u0627\u0641\u0629 \u0645\u0646 DMSO\u060c \u062b\u0645 \u0627\u0642\u0633\u0645 \u0639\u0644\u0649 \u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0644\u0644\u0645\u0632\u064a\u062c.<\/p>\n<p>\u0627\u0644\u0645\u062e\u0632\u0648\u0646 \u0627\u0644\u0645\u064f\u062d\u0636\u064e\u0651\u0631 \u0641\u064a DMSO \u0627\u0644\u0646\u0642\u064a \u064a\u0633\u0627\u0647\u0645 \u0628\u0645\u064a\u0643\u0631\u0648\u0644\u062a\u0631 \u0648\u0627\u062d\u062f \u0645\u0646 DMSO \u0644\u0643\u0644 \u0645\u064a\u0643\u0631\u0648\u0644\u062a\u0631 \u064a\u064f\u0646\u0642\u0644. \u0627\u0644\u0645\u062e\u0632\u0648\u0646 \u0627\u0644\u0630\u064a \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 20% DMSO \u064a\u0633\u0627\u0647\u0645 \u0628\u0623\u0642\u0644 \u0645\u0646 \u0630\u0644\u0643.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t05\/en\/1.png\" alt=\"Figure 1. A transfer carries both peptide and solvent into the final mixture.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 1. \u0643\u0644 \u0645\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0648\u0627\u0644\u0645\u0630\u064a\u0628 \u064a\u0646\u062a\u0642\u0644\u0627\u0646 \u0645\u0639 \u0627\u0644\u062d\u0635\u0629 \u0627\u0644\u0645\u0623\u062e\u0648\u0630\u0629 \u0645\u0646 \u0627\u0644\u0645\u062e\u0632\u0648\u0646.<\/figcaption><\/figure>\n<h2>\u0645\u062b\u0627\u0644 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 DMSO \u0627\u0644\u0646\u0642\u064a<\/h2>\n<p>Assume a 1 mM peptide stock in pure DMSO. Transfer 10 \u00b5L and make the final mixture up to 1,000 \u00b5L. The peptide concentration is 1,000 \u00b5M \u00d7 10 \u00f7 1,000 = 10 \u00b5M.<\/p>\n<p>The DMSO percentage is 10 \u00f7 1,000 \u00d7 100% = 1% by volume. These numbers describe the same mixture, but one describes peptide concentration and the other solvent proportion.<\/p>\n<p class=\"formula\">10 \u00b5L DMSO \u00f7 1,000 \u00b5L final volume \u00d7 100% = 1%<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t05\/en\/2.png\" alt=\"Figure 2. 10 \u00b5L of a stock in 100% DMSO made up to 1,000 \u00b5L gives 1% DMSO by volume.\"><figcaption>Figure 2. In this example, 10 \u00b5L of pure DMSO in a final 1,000 \u00b5L gives 1% DMSO.<\/figcaption><\/figure>\n<h2>\u0645\u0627 \u0627\u0644\u0630\u064a \u064a\u062a\u063a\u064a\u0631 \u0625\u0630\u0627 \u0643\u0627\u0646 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0645\u062e\u0632\u0651\u0646 \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 20% DMSO\u061f<\/h2>\n<p>The same 10 \u00b5L aliquot now carries 10 \u00d7 0.20 = 2 \u00b5L of DMSO. In a final 1,000 \u00b5L mixture, that is 0.2% by volume.<\/p>\n<p>\u0625\u0630\u0627 \u0627\u062d\u062a\u0648\u062a \u0639\u062f\u0629 \u0643\u0648\u0627\u0634\u0641 \u0639\u0644\u0649 DMSO\u060c \u0641\u0627\u062c\u0645\u0639 \u062c\u0645\u064a\u0639 \u0625\u0633\u0647\u0627\u0645\u0627\u062a DMSO \u0642\u0628\u0644 \u0627\u0644\u0642\u0633\u0645\u0629 \u0639\u0644\u0649 \u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a. \u0644\u0627 \u062a\u062d\u0633\u0628 \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u062e\u0632\u0651\u0646 \u0641\u0642\u0637.<\/p>\n<h2>\u0644\u0645\u0627\u0630\u0627 \u064a\u0647\u0645 \u0636\u0627\u0628\u0637 \u0627\u0644\u0645\u0630\u064a\u0628<\/h2>\n<p>\u0625\u0630\u0627 \u062a\u0644\u0642\u062a \u0627\u0644\u0622\u0628\u0627\u0631 \u0623\u062d\u062c\u0627\u0645\u064b\u0627 \u0645\u062e\u062a\u0644\u0641\u0629 \u0645\u0646 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0645\u062e\u0632\u0651\u0646\u060c \u0641\u0642\u062f \u062a\u062e\u062a\u0644\u0641 \u0646\u0633\u0628 DMSO \u0641\u064a\u0647\u0627 \u0623\u064a\u0636\u064b\u0627. \u064a\u0633\u0627\u0639\u062f\u0643 \u0636\u0627\u0628\u0637 \u0627\u0644\u0645\u0630\u064a\u0628 \u0627\u0644\u0645\u0646\u0627\u0633\u0628 \u0639\u0644\u0649 \u0627\u0633\u062a\u0642\u0635\u0627\u0621 \u0625\u0633\u0647\u0627\u0645 \u0627\u0644\u0645\u0630\u064a\u0628 \u0628\u062f\u0644\u064b\u0627 \u0645\u0646 \u0639\u0632\u0648 \u0643\u0644 \u062a\u063a\u064a\u064a\u0631 \u0625\u0644\u0649 \u0627\u0644\u0628\u0628\u062a\u064a\u062f.<\/p>\n<p>\u062a\u0639\u062a\u0645\u062f \u0645\u0642\u0628\u0648\u0644\u064a\u0629 \u0646\u0633\u0628\u0629 \u0645\u0639\u064a\u0646\u0629 \u0639\u0644\u0649 \u0646\u0638\u0627\u0645 \u0627\u0644\u0628\u062d\u062b. \u0625\u0646 1% \u0648 0.2% \u0647\u0646\u0627 \u0645\u062c\u0631\u062f \u062d\u0633\u0627\u0628\u0627\u062a\u060c \u0648\u0644\u064a\u0633\u062a \u062d\u062f\u0648\u062f\u064b\u0627 \u0645\u0648\u0635\u0649 \u0628\u0647\u0627.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t05\/en\/3.png\" alt=\"Figure 3. Track solvent fraction alongside the peptide concentration at every level.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 3. \u0627\u062d\u0633\u0628 \u0627\u0644\u062a\u0639\u0631\u0636 \u0644\u0644\u0645\u0630\u064a\u0628 \u0625\u0644\u0649 \u062c\u0627\u0646\u0628 \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0628\u0628\u062a\u064a\u062f.<\/figcaption><\/figure>\n<h2>\u062c\u0631\u0651\u0628 \u062d\u062c\u0645\u064b\u0627 \u0645\u0623\u062e\u0648\u0630\u064b\u0627 \u0645\u0646 \u0645\u062d\u0644\u0648\u0644 \u0645\u062e\u0632\u0648\u0646 \u0628\u0645\u0630\u064a\u0628 \u0645\u062e\u062a\u0644\u0637<\/h2>\n<div class=\"exercise\"><strong>Transfer 5 \u00b5L of a stock containing 40% DMSO into a final 1,000 \u00b5L. What is the final DMSO percentage?<\/strong><\/p>\n<details>\n<summary>\u0639\u0631\u0636 \u0627\u0644\u0625\u062c\u0627\u0628\u0629<\/summary>\n<p>The aliquot contains 5 \u00d7 0.40 = 2 \u00b5L of DMSO. The final percentage is 2 \u00f7 1,000 \u00d7 100% = 0.2% by volume.<\/p>\n<\/details>\n<\/div>\n<p>\u0627\u0643\u062a\u0628 \u062a\u0631\u0643\u064a\u0628 \u0645\u0630\u064a\u0628 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0645\u062e\u0632\u0648\u0646 \u0648\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0628\u062c\u0627\u0646\u0628 \u0627\u0644\u062d\u0633\u0627\u0628. \u062d\u062c\u0645 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0645\u062e\u0632\u0648\u0646 \u0648\u062d\u062f\u0647 \u0644\u0627 \u064a\u0643\u0641\u064a \u0644\u0625\u0639\u0627\u062f\u0629 \u0625\u0646\u062a\u0627\u062c\u0647.<\/p>\n<h2>\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u0645\u0630\u064a\u0628 \u0639\u0646\u062f \u0627\u0644\u062a\u062e\u0637\u064a\u0637 \u0644\u0623\u0639\u0645\u0627\u0644 LL-37 \u0623\u0648 SS-31<\/h2>\n<p>\u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0625\u0644\u0649 LL-37 \u0623\u0648 SS-31\u060c \u062a\u0623\u0643\u062f \u0645\u0646 \u0627\u0644\u0645\u0630\u064a\u0628 \u0627\u0644\u0645\u0646\u0627\u0633\u0628 \u0644\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u0645\u0648\u0631\u062f\u0629. \u062b\u0645 \u0627\u062d\u0633\u0628 \u0645\u0627 \u064a\u0633\u0647\u0645 \u0628\u0647 \u0630\u0644\u0643 \u0627\u0644\u0645\u0630\u064a\u0628 \u0641\u064a \u0627\u0644\u0645\u0632\u064a\u062c \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0627\u0644\u0645\u062e\u0637\u0637 \u0644\u0647. \u0627\u0633\u0645 \u0627\u0644\u0645\u0646\u062a\u062c \u0644\u0627 \u064a\u062b\u0628\u062a \u0637\u0631\u064a\u0642\u0629 \u062a\u062d\u0636\u064a\u0631 \u0639\u0627\u0644\u0645\u064a\u0629 \u0644\u0640 DMSO.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/37\/\">LL-37<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/ss-31-%d8%a5%d9%8a%d9%84%d8%a7%d9%85%d9%8a%d8%a8%d8%b1%d9%8a%d8%aa%d9%8a%d8%af\/\">SS-31<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%82%d8%aa%d8%a8%d8%a7%d8%b3\/\">\u0646\u0627\u0642\u0634 \u0645\u062a\u0637\u0644\u0628\u0627\u062a \u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0645\u0633\u062a\u0646\u062f\u0627\u062a \u0645\u0639 NHD<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d8%b7%d8%b1%d9%82-%d8%a7%d9%84%d8%aa%d8%ad%d9%84%d9%8a%d9%84%d9%8a%d8%a9\/%d9%85%d8%ad%d8%aa%d9%88%d9%89-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af-%d9%85%d9%82%d8%a7%d8%a8%d9%84-%d8%af%d8%b1%d8%ac%d8%a9-%d8%a7%d9%84%d9%86%d9%82%d8%a7%d8%a1\/\">\u062a\u0627\u0628\u0639 \u0645\u0639 \u0627\u0644\u062f\u0644\u064a\u0644 \u0627\u0644\u062a\u062d\u0644\u064a\u0644\u064a<\/a><\/p>\n<footer><strong>\u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0648\u0645\u0644\u0627\u062d\u0638\u0627\u062a \u0627\u0644\u0623\u0645\u062b\u0644\u0629<\/strong><\/p>\n<p>\u0627\u0644\u0623\u0631\u0642\u0627\u0645 \u0648\u0627\u0644\u0645\u0646\u062d\u0646\u064a\u0627\u062a \u0623\u0645\u062b\u0644\u0629 \u062a\u0639\u0644\u064a\u0645\u064a\u0629\u060c \u0648\u0644\u064a\u0633\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 \u062f\u0641\u0639\u0629 \u0645\u0646\u062a\u062c. \u062a\u0645 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0641\u064a 11 \u0623\u0643\u062a\u0648\u0628\u0631 2026.<\/p>\n<ul>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/support\/calculators-and-apps\/solution-dilution-calculator\">Sigma-Aldrich \u00b7 solution dilution calculator<\/a><\/li>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/content\/dam\/sigma-aldrich\/docs\/Sigma\/General_Information\/peptide_handling_guide.pdf\">Sigma-Aldrich \u00b7 peptide handling guide<\/a><\/li>\n<\/ul>\n<p>\u0627\u0644\u0643\u062a\u0627\u0628\u0629 \u0628\u0645\u0633\u0627\u0639\u062f\u0629 \u0627\u0644\u0630\u0643\u0627\u0621 \u0627\u0644\u0627\u0635\u0637\u0646\u0627\u0639\u064a \u0648\u0645\u0631\u0627\u062c\u0639\u0629 \u0627\u0644\u0645\u0635\u0627\u062f\u0631. \u0645\u0648\u0627\u062f \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c \u0645\u062e\u0635\u0635\u0629 \u0644\u0644\u0628\u062d\u062b \u0648\u0627\u0644\u062a\u0637\u0648\u064a\u0631 \u0648\u0627\u0644\u062a\u0635\u0646\u064a\u0639\u060c \u0648\u0644\u064a\u0633 \u0644\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0634\u0631\u064a \u0623\u0648 \u0627\u0644\u0628\u064a\u0637\u0631\u064a.<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u062a\u062a\u0628\u064e\u0651\u0639 \u0645\u0642\u062f\u0627\u0631 DMSO \u0627\u0644\u0630\u064a \u064a\u062f\u062e\u0644 \u0627\u0644\u062e\u0644\u064a\u0637 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0639\u0646\u062f \u062a\u062e\u0641\u064a\u0641 \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0623\u0633\u0627\u0633\u064a.<\/p>","protected":false},"author":1,"featured_media":3057,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and clearly labeled examples","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Reference data and teaching examples are separated from NHD lot results; product specifications and availability require confirmation.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"AI-assisted drafting, source checks, translation and generated comic illustration. This is not independent peer review or supplied-lot verification.","pim_sources":"[{\"title\":\"Thermo Fisher: handling and storage instructions for standard peptides\",\"note\":\"Thermo Fisher: handling and storage instructions for standard peptides. 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