{"id":3081,"date":"2026-10-03T20:25:51","date_gmt":"2026-10-03T20:25:51","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3081"},"modified":"2026-10-03T20:25:51","modified_gmt":"2026-10-03T20:25:51","slug":"%d9%85%d8%ab%d8%a7%d9%84-%d8%a8%d8%b3%d9%8a%d8%b7-%d9%84%d8%ad%d8%a7%d9%84%d8%a9-%d8%b4%d8%ad%d9%86%d8%a9-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af-%d9%81%d9%8a-%d9%85%d8%b7%d9%8a%d8%a7%d9%81-%d8%a7","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ar\/peptide-lcms-charge-state-mz-simple-example\/","title":{"rendered":"\u0644\u0645\u0627\u0630\u0627 \u064a\u0638\u0647\u0631 \u0637\u064a\u0641 \u0643\u062a\u0644\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0646\u0635\u0641 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0645\u062a\u0648\u0642\u0639\u0629\u061f"},"content":{"rendered":"<p>\u062a\u0633\u0631\u062f \u0648\u062b\u064a\u0642\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0643 \u0643\u062a\u0644\u0629 \u062c\u0632\u064a\u0626\u064a\u0629 \u0642\u0631\u064a\u0628\u0629 \u0645\u0646 2,000 Da\u060c \u0644\u0643\u0646 \u0627\u0644\u0637\u064a\u0641 \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0642\u0645\u0629 \u0642\u0631\u064a\u0628\u0629 \u0645\u0646 1,001. \u0647\u0644 \u0623\u0631\u0633\u0644 \u0627\u0644\u0645\u0648\u0631\u062f \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u062e\u0637\u0623\u061f \u0647\u0630\u0627 \u0627\u0644\u0631\u0642\u0645 \u0648\u062d\u062f\u0647 \u0644\u0627 \u064a\u0645\u0643\u0646\u0647 \u0627\u0644\u0625\u062c\u0627\u0628\u0629 \u0639\u0646 \u0627\u0644\u0633\u0624\u0627\u0644. \u064a\u0642\u064a\u0633 \u0645\u0637\u064a\u0627\u0641 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0643\u062a\u0644\u0629 \u0645\u0642\u0633\u0648\u0645\u0629 \u0639\u0644\u0649 \u0627\u0644\u0634\u062d\u0646\u0629\u060c \u0648\u064a\u064f\u0643\u062a\u0628 \u0639\u0627\u062f\u0629\u064b m\/z.<\/p>\n<h2>\u064a\u0645\u0643\u0646 \u0644\u062c\u0632\u064a\u0621 \u0648\u0627\u062d\u062f \u0623\u0646 \u064a\u062d\u0645\u0644 \u0623\u0643\u062b\u0631 \u0645\u0646 \u0634\u062d\u0646\u0629 \u0648\u0627\u062d\u062f\u0629<\/h2>\n<p>Think of the instrument\u2019s number as a divided value. In positive electrospray, a peptide can pick up more than one proton. Two charges divide the ion\u2019s mass by two; three divide it by three. Thermo Fisher\u2019s introduction explains why mass spectrometry reports mass-to-charge values rather than a simple weight.<\/p>\n<h2>\u0645\u062b\u0627\u0644 \u0645\u062d\u0644\u0648\u0644: 2,000 Da \u064a\u0635\u0628\u062d \u062d\u0648\u0627\u0644\u064a 1,001 m\/z<\/h2>\n<p>Take an imaginary peptide with a neutral mass of 2,000 Da. For proton-only positive ions, use m\/z = (M + z \u00d7 1.0073) \u00f7 z, where M is neutral mass and z is charge. The approximate proton mass is 1.0073 Da. At charge +1, the result is 2,001.0073; at +2, it is 1,001.0073; at +3, it is 667.6739.<\/p>\n<p>\u0647\u0630\u0647 \u0642\u064a\u0645 \u062a\u0639\u0644\u064a\u0645\u064a\u0629 \u0645\u062d\u0633\u0648\u0628\u0629\u060c \u0648\u0644\u064a\u0633\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 NHD. \u0648\u0647\u064a \u062a\u0635\u0641 \u062b\u0644\u0627\u062b \u062d\u0627\u0644\u0627\u062a \u0634\u062d\u0646\u0629 \u0644\u0644\u062c\u0632\u064a\u0621 \u0627\u0644\u0627\u0641\u062a\u0631\u0627\u0636\u064a \u0646\u0641\u0633\u0647. \u0627\u0644\u0631\u0642\u0645 \u0627\u0644\u0623\u0635\u063a\u0631 \u0644\u0627 \u064a\u0639\u0646\u064a \u0623\u0646 \u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0646\u0642\u0633\u0645\u062a \u0625\u0644\u0649 \u0646\u0635\u0641\u064a\u0646.<\/p>\n<h2>\u0627\u0633\u0623\u0644 \u0639\u0646 \u0627\u0644\u0631\u0642\u0645 \u0627\u0644\u0630\u064a \u064a\u0639\u0631\u0636\u0647 \u0627\u0644\u062a\u0642\u0631\u064a\u0631<\/h2>\n<p>\u062a\u062d\u0642\u0642 \u0645\u0646 \u062a\u0639\u064a\u064a\u0646 \u0627\u0644\u0634\u062d\u0646\u0629 \u0648\u0645\u0627 \u0625\u0630\u0627 \u0643\u0627\u0646 \u0627\u0644\u062a\u0642\u0631\u064a\u0631 \u064a\u0639\u0637\u064a m\/z \u062e\u0627\u0645\u064b\u0627 \u0623\u0648 \u0643\u062a\u0644\u0629 \u0645\u062d\u0627\u064a\u062f\u0629 \u0645\u0639\u0627\u062f \u0628\u0646\u0627\u0624\u0647\u0627. \u0627\u0633\u0623\u0644 \u0623\u064a\u0636\u064b\u0627 \u0645\u0627 \u0625\u0630\u0627 \u0643\u0627\u0646 \u064a\u0633\u062a\u062e\u062f\u0645 \u0627\u0644\u0643\u062a\u0644\u0629 \u0623\u062d\u0627\u062f\u064a\u0629 \u0627\u0644\u0646\u0638\u064a\u0631 \u0623\u0648 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0645\u062a\u0648\u0633\u0637\u0629. \u0647\u0627\u062a\u0627\u0646 \u0637\u0631\u064a\u0642\u062a\u0627\u0646 \u0645\u062e\u062a\u0644\u0641\u062a\u0627\u0646 \u0644\u062a\u0644\u062e\u064a\u0635 \u0627\u0644\u0646\u0638\u0627\u0626\u0631. \u062a\u062d\u062a\u0627\u062c \u0625\u0636\u0627\u0641\u0627\u062a \u0627\u0644\u0635\u0648\u062f\u064a\u0648\u0645 \u0648\u0623\u0634\u0643\u0627\u0644 \u0627\u0644\u0623\u064a\u0648\u0646\u0627\u062a \u0627\u0644\u0623\u062e\u0631\u0649 \u0648\u0627\u0644\u0634\u0638\u0627\u064a\u0627 \u0625\u0644\u0649 \u062a\u0641\u0633\u064a\u0631 \u0645\u062e\u062a\u0644\u0641\u061b \u0641\u0627\u0644\u0635\u064a\u063a\u0629 \u0627\u0644\u0628\u0633\u064a\u0637\u0629 \u0627\u0644\u0645\u0639\u062a\u0645\u062f\u0629 \u0639\u0644\u0649 \u0627\u0644\u0628\u0631\u0648\u062a\u0648\u0646 \u0641\u0642\u0637 \u0644\u0627 \u062a\u063a\u0637\u064a\u0647\u0627.<\/p>\n<h2>\u062a\u0637\u0627\u0628\u0642 \u0627\u0644\u0643\u062a\u0644\u0629 \u0645\u0641\u064a\u062f\u060c \u0644\u0643\u0646\u0647 \u0644\u064a\u0633 \u0641\u062d\u0635 \u0627\u0644\u062c\u0648\u062f\u0629 \u0627\u0644\u0643\u0627\u0645\u0644<\/h2>\n<p>\u062a\u062f\u0639\u0645 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0645\u0637\u0627\u0628\u0642\u0629 \u0627\u0644\u0647\u0648\u064a\u0629 \u0627\u0644\u0645\u0642\u062a\u0631\u062d\u0629. \u0644\u0643\u0646\u0647\u0627 \u0644\u0627 \u062a\u062b\u0628\u062a \u0628\u062d\u062f \u0630\u0627\u062a\u0647\u0627 \u062a\u0631\u062a\u064a\u0628 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0623\u0648 \u0627\u0644\u0646\u0642\u0627\u0621 \u0623\u0648 \u0643\u0645\u064a\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0641\u064a \u0627\u0644\u0645\u0633\u062d\u0648\u0642. \u0627\u0633\u062a\u062e\u062f\u0645 \u0646\u062a\u064a\u062c\u0629 \u0627\u0644\u0643\u062a\u0644\u0629 \u062c\u0646\u0628\u064b\u0627 \u0625\u0644\u0649 \u062c\u0646\u0628 \u0645\u0639 \u0627\u0644\u0633\u062c\u0644\u0627\u062a \u0627\u0644\u062a\u062d\u0644\u064a\u0644\u064a\u0629 \u0627\u0644\u0645\u062a\u0641\u0642 \u0639\u0644\u064a\u0647\u0627. \u0644\u062f\u064a\u0646\u0627 <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d8%b7%d8%b1%d9%82-%d8%a7%d9%84%d8%aa%d8%ad%d9%84%d9%8a%d9%84%d9%8a%d8%a9\/%d8%a7%d8%ae%d8%aa%d8%a8%d8%a7%d8%b1-%d9%87%d9%88%d9%8a%d8%a9-lc-ms\/\">\u062f\u0644\u064a\u0644 \u062a\u062d\u062f\u064a\u062f \u0627\u0644\u0647\u0648\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 LC\u2013MS<\/a> \u064a\u0634\u0631\u062d \u0647\u0630\u0627 \u0627\u0644\u062a\u0645\u064a\u064a\u0632.<\/p>\n<p>\u0647\u0644 \u062a\u0634\u062a\u0631\u064a \u0645\u0627\u062f\u0629 \u0645\u0646 \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c \u0645\u062b\u0644 <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/37\/\">LL-37<\/a>\u060c \u0623\u0648 \u062a\u0637\u0644\u0628 \u062a\u0633\u0644\u0633\u0644\u064b\u0627 \u0645\u062e\u0635\u0635\u064b\u0627\u061f <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%82%d8%aa%d8%a8%d8%a7%d8%b3\/\">\u0623\u0631\u0633\u0644 \u0625\u0644\u0649 NHD \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u062f\u0642\u064a\u0642\u0629 \u0648\u0627\u0633\u0623\u0644 \u0639\u0646 \u0633\u062c\u0644\u0627\u062a \u0627\u0644\u0647\u0648\u064a\u0629 \u0627\u0644\u0645\u062a\u0627\u062d\u0629<\/a>. Confirm the scope before ordering; this example does not establish any supplied lot\u2019s identity.<\/p>\n<p>\u0644\u0623\u063a\u0631\u0627\u0636 \u0627\u0644\u0628\u062d\u062b \u0627\u0644\u0645\u062e\u0628\u0631\u064a \u0641\u0642\u0637. \u063a\u064a\u0631 \u0645\u062e\u0635\u0635 \u0644\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0634\u0631\u064a \u0623\u0648 \u0627\u0644\u0628\u064a\u0637\u0631\u064a.<\/p>\n<h2>\u0645\u0631\u0627\u062c\u0639<\/h2>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/pl\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\">Thermo Fisher: \u0646\u0638\u0631\u0629 \u0639\u0627\u0645\u0629 \u0639\u0644\u0649 \u0645\u0637\u064a\u0627\u0641 \u0627\u0644\u0643\u062a\u0644\u0629<\/a><\/li>\n<li><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/posters\/po-80036-asms22-charge-assignment-of-isotopically-po80036-en.pdf\">Thermo Fisher: \u062a\u0639\u064a\u064a\u0646 \u0627\u0644\u0634\u062d\u0646\u0629 \u0644\u0628\u064a\u0627\u0646\u0627\u062a \u0627\u0644\u0643\u062a\u0644\u0629 \u0645\u062d\u0644\u0648\u0644\u0629 \u0627\u0644\u0646\u0638\u0627\u0626\u0631<\/a><\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p>\u062a\u0633\u0631\u062f \u0648\u062b\u064a\u0642\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0643 \u0643\u062a\u0644\u0629 \u062c\u0632\u064a\u0626\u064a\u0629 \u0642\u0631\u064a\u0628\u0629 \u0645\u0646 2,000 Da\u060c \u0644\u0643\u0646 \u0627\u0644\u0637\u064a\u0641 \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0642\u0645\u0629 \u0642\u0631\u064a\u0628\u0629 \u0645\u0646 1,001. \u0647\u0644 \u0623\u0631\u0633\u0644 \u0627\u0644\u0645\u0648\u0631\u062f \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u062e\u0637\u0623\u061f \u0647\u0630\u0627 \u0627\u0644\u0631\u0642\u0645 \u0648\u062d\u062f\u0647 \u0644\u0627 \u064a\u0645\u0643\u0646\u0647 \u0627\u0644\u0625\u062c\u0627\u0628\u0629 \u0639\u0646 \u0627\u0644\u0633\u0624\u0627\u0644. \u064a\u0642\u064a\u0633 \u0645\u0637\u064a\u0627\u0641 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0643\u062a\u0644\u0629 \u0645\u0642\u0633\u0648\u0645\u0629 \u0639\u0644\u0649 \u0627\u0644\u0634\u062d\u0646\u0629\u060c \u0648\u064a\u064f\u0643\u062a\u0628 \u0639\u0627\u062f\u0629\u064b m\/z.<\/p>","protected":false},"author":1,"featured_media":3083,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and labeled calculations","pim_reviewer":"Codex AI editorial","pim_reviewed_date":"2026-10-04","pim_editorial_note":"Examples are calculations, not NHD lot results. Confirm project feasibility and document availability.","pim_review_status":"reviewed","pim_review_reason":"Source, arithmetic and search-intent checks; no independent human technical review recorded.","pim_creation_method":"AI-assisted writing, source checks and illustrated cover.","pim_sources":"[{\"title\":\"Thermo Fisher: Overview of mass spectrometry\",\"url\":\"https:\/\/www.thermofisher.com\/pl\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\",\"note\":\"Technical reference; numerical planning examples are original calculations.\"},{\"title\":\"Thermo Fisher: Charge assignment of isotopically resolved mass data\",\"url\":\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/posters\/po-80036-asms22-charge-assignment-of-isotopically-po80036-en.pdf\",\"note\":\"Technical reference; numerical planning examples are original calculations.\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3081","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Why does a peptide mass spectrum show half the expected mass? - NHD<\/title>\n<meta name=\"description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ar\/\u0645\u062b\u0627\u0644-\u0628\u0633\u064a\u0637-\u0644\u062d\u0627\u0644\u0629-\u0634\u062d\u0646\u0629-\u0627\u0644\u0628\u0628\u062a\u064a\u062f-\u0641\u064a-\u0645\u0637\u064a\u0627\u0641-\u0627\/\" \/>\n<meta property=\"og:locale\" content=\"ar_AR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Why does a peptide mass spectrum show half the expected mass?\" \/>\n<meta property=\"og:description\" content=\"Your peptide document lists a molecular mass near 2,000 Da, but the spectrum has a peak near 1,001. Has the supplier sent the wrong material? That number alone cannot answer the question. A mass spectrometer measures mass divided by charge, usually written as m\/z.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nhdbio.com\/ar\/\u0645\u062b\u0627\u0644-\u0628\u0633\u064a\u0637-\u0644\u062d\u0627\u0644\u0629-\u0634\u062d\u0646\u0629-\u0627\u0644\u0628\u0628\u062a\u064a\u062f-\u0641\u064a-\u0645\u0637\u064a\u0627\u0641-\u0627\/\" \/>\n<meta property=\"og:site_name\" content=\"NHD\" \/>\n<meta property=\"article:published_time\" content=\"2026-10-03T20:25:51+00:00\" \/>\n<meta name=\"author\" content=\"NHD Technical Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"twitter:label1\" content=\"\u0643\u064f\u062a\u0628 \u0628\u0648\u0627\u0633\u0637\u0629\" \/>\n\t<meta name=\"twitter:data1\" content=\"NHD Technical Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u0648\u0642\u062a \u0627\u0644\u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0645\u064f\u0642\u062f\u0651\u0631\" \/>\n\t<meta name=\"twitter:data2\" content=\"\u062f\u0642\u064a\u0642\u062a\u0627\u0646\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\"},\"author\":{\"name\":\"NHD Technical Team\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/person\\\/0ffb0dec7fda0f720073fc468fac6fc1\"},\"headline\":\"Why does a peptide mass spectrum show half the expected mass?\",\"datePublished\":\"2026-10-03T20:25:51+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\"},\"wordCount\":378,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/charge-cover.png\",\"articleSection\":[\"Evidence &amp; Quality Guides\"],\"inLanguage\":\"ar\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\",\"url\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\",\"name\":\"Why does a peptide mass spectrum show half the expected mass? - NHD\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/charge-cover.png\",\"datePublished\":\"2026-10-03T20:25:51+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#breadcrumb\"},\"inLanguage\":\"ar\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"ar\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#primaryimage\",\"url\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/charge-cover.png\",\"contentUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/charge-cover.png\",\"width\":1536,\"height\":1024,\"caption\":\"Illustration; not an experimental spectrum.\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/nhdbio.com\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Why does a peptide mass spectrum show half the expected mass?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#website\",\"url\":\"https:\\\/\\\/nhdbio.com\\\/\",\"name\":\"NHD\",\"description\":\"Peptide Raw Materials and Custom Peptide Synthesis\",\"publisher\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/nhdbio.com\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"ar\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\",\"name\":\"NHD\",\"url\":\"https:\\\/\\\/nhdbio.com\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ar\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/09\\\/codex-clipboard-354c928e-4b5b-482d-9261-02023d4c9680.png\",\"contentUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/09\\\/codex-clipboard-354c928e-4b5b-482d-9261-02023d4c9680.png\",\"width\":2172,\"height\":724,\"caption\":\"NHD\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/logo\\\/image\\\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/person\\\/0ffb0dec7fda0f720073fc468fac6fc1\",\"name\":\"NHD Technical Team\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"ar\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8445618b3e2834fa0c7ae1a03a371ed002e9bf8d2cc1cd989dc4a71bb2b66110?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8445618b3e2834fa0c7ae1a03a371ed002e9bf8d2cc1cd989dc4a71bb2b66110?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/8445618b3e2834fa0c7ae1a03a371ed002e9bf8d2cc1cd989dc4a71bb2b66110?s=96&d=mm&r=g\",\"caption\":\"NHD Technical Team\"},\"description\":\"NHD Technical Team maintains research-use catalog documentation and evidence summaries. Reviewed articles link to peer-reviewed papers, trial registries and regulatory records, retain null findings and study limitations, and distinguish published interventions from catalog materials. AI-assisted drafting may be used for structure; source links and evidence boundaries remain visible. This work is not independent peer review or medical advice.\",\"sameAs\":[\"https:\\\/\\\/nhdbio.com\"],\"url\":\"https:\\\/\\\/nhdbio.com\\\/ar\\\/author\\\/qianmiao-technical-team\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"\u0644\u0645\u0627\u0630\u0627 \u064a\u064f\u0638\u0647\u0631 \u0645\u0637\u064a\u0627\u0641 \u0643\u062a\u0644\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0646\u0635\u0641 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0645\u062a\u0648\u0642\u0639\u0629\u061f - NHD","description":"\u0645\u0648\u0627\u062f \u062e\u0627\u0645 \u0628\u0628\u062a\u064a\u062f\u064a\u0629 \u0648\u062a\u062e\u0644\u064a\u0642 \u0627\u0644\u0628\u0628\u062a\u064a\u062f\u0627\u062a \u062d\u0633\u0628 \u0627\u0644\u0637\u0644\u0628","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/nhdbio.com\/ar\/\u0645\u062b\u0627\u0644-\u0628\u0633\u064a\u0637-\u0644\u062d\u0627\u0644\u0629-\u0634\u062d\u0646\u0629-\u0627\u0644\u0628\u0628\u062a\u064a\u062f-\u0641\u064a-\u0645\u0637\u064a\u0627\u0641-\u0627\/","og_locale":"ar_AR","og_type":"article","og_title":"Why does a peptide mass spectrum show half the expected mass?","og_description":"Your peptide document lists a molecular mass near 2,000 Da, but the spectrum has a peak near 1,001. Has the supplier sent the wrong material? That number alone cannot answer the question. A mass spectrometer measures mass divided by charge, usually written as m\/z.","og_url":"https:\/\/nhdbio.com\/ar\/\u0645\u062b\u0627\u0644-\u0628\u0633\u064a\u0637-\u0644\u062d\u0627\u0644\u0629-\u0634\u062d\u0646\u0629-\u0627\u0644\u0628\u0628\u062a\u064a\u062f-\u0641\u064a-\u0645\u0637\u064a\u0627\u0641-\u0627\/","og_site_name":"NHD","article_published_time":"2026-10-03T20:25:51+00:00","author":"NHD Technical Team","twitter_card":"summary_large_image","twitter_description":"Peptide Raw Materials and Custom Peptide Synthesis","twitter_misc":{"\u0643\u064f\u062a\u0628 \u0628\u0648\u0627\u0633\u0637\u0629":"NHD Technical Team","\u0648\u0642\u062a \u0627\u0644\u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0645\u064f\u0642\u062f\u0651\u0631":"\u062f\u0642\u064a\u0642\u062a\u0627\u0646"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#article","isPartOf":{"@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/"},"author":{"name":"NHD Technical Team","@id":"https:\/\/nhdbio.com\/#\/schema\/person\/0ffb0dec7fda0f720073fc468fac6fc1"},"headline":"Why does a peptide mass spectrum show half the expected mass?","datePublished":"2026-10-03T20:25:51+00:00","mainEntityOfPage":{"@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/"},"wordCount":378,"commentCount":0,"publisher":{"@id":"https:\/\/nhdbio.com\/#organization"},"image":{"@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#primaryimage"},"thumbnailUrl":"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/charge-cover.png","articleSection":["Evidence &amp; Quality Guides"],"inLanguage":"ar","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/","url":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/","name":"\u0644\u0645\u0627\u0630\u0627 \u064a\u064f\u0638\u0647\u0631 \u0645\u0637\u064a\u0627\u0641 \u0643\u062a\u0644\u0629 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0646\u0635\u0641 \u0627\u0644\u0643\u062a\u0644\u0629 \u0627\u0644\u0645\u062a\u0648\u0642\u0639\u0629\u061f - NHD","isPartOf":{"@id":"https:\/\/nhdbio.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#primaryimage"},"image":{"@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#primaryimage"},"thumbnailUrl":"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/charge-cover.png","datePublished":"2026-10-03T20:25:51+00:00","breadcrumb":{"@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#breadcrumb"},"inLanguage":"ar","potentialAction":[{"@type":"ReadAction","target":["https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/"]}]},{"@type":"ImageObject","inLanguage":"ar","@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#primaryimage","url":"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/charge-cover.png","contentUrl":"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/charge-cover.png","width":1536,"height":1024,"caption":"Illustration; not an experimental spectrum."},{"@type":"BreadcrumbList","@id":"https:\/\/nhdbio.com\/peptide-lcms-charge-state-mz-simple-example\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/nhdbio.com\/"},{"@type":"ListItem","position":2,"name":"Why does a peptide mass spectrum show half the expected mass?"}]},{"@type":"WebSite","@id":"https:\/\/nhdbio.com\/#website","url":"https:\/\/nhdbio.com\/","name":"NHD","description":"\u0645\u0648\u0627\u062f \u062e\u0627\u0645 \u0628\u0628\u062a\u064a\u062f\u064a\u0629 \u0648\u062a\u062e\u0644\u064a\u0642 \u0627\u0644\u0628\u0628\u062a\u064a\u062f\u0627\u062a \u062d\u0633\u0628 \u0627\u0644\u0637\u0644\u0628","publisher":{"@id":"https:\/\/nhdbio.com\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/nhdbio.com\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"ar"},{"@type":"Organization","@id":"https:\/\/nhdbio.com\/#organization","name":"NHD","url":"https:\/\/nhdbio.com\/","logo":{"@type":"ImageObject","inLanguage":"ar","@id":"https:\/\/nhdbio.com\/#\/schema\/logo\/image\/","url":"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/09\/codex-clipboard-354c928e-4b5b-482d-9261-02023d4c9680.png","contentUrl":"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/09\/codex-clipboard-354c928e-4b5b-482d-9261-02023d4c9680.png","width":2172,"height":724,"caption":"NHD"},"image":{"@id":"https:\/\/nhdbio.com\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/nhdbio.com\/#\/schema\/person\/0ffb0dec7fda0f720073fc468fac6fc1","name":"\u0627\u0644\u0641\u0631\u064a\u0642 \u0627\u0644\u062a\u0642\u0646\u064a \u0641\u064a NHD","image":{"@type":"ImageObject","inLanguage":"ar","@id":"https:\/\/secure.gravatar.com\/avatar\/8445618b3e2834fa0c7ae1a03a371ed002e9bf8d2cc1cd989dc4a71bb2b66110?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/8445618b3e2834fa0c7ae1a03a371ed002e9bf8d2cc1cd989dc4a71bb2b66110?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8445618b3e2834fa0c7ae1a03a371ed002e9bf8d2cc1cd989dc4a71bb2b66110?s=96&d=mm&r=g","caption":"NHD Technical Team"},"description":"\u064a\u062d\u062a\u0641\u0638 \u0641\u0631\u064a\u0642 NHD \u0627\u0644\u0641\u0646\u064a \u0628\u0648\u062b\u0627\u0626\u0642 \u0643\u062a\u0627\u0644\u0648\u062c \u0627\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u062d\u062b\u064a \u0648\u0645\u0644\u062e\u0635\u0627\u062a \u0627\u0644\u0623\u062f\u0644\u0629. \u062a\u0631\u062a\u0628\u0637 \u0627\u0644\u0645\u0642\u0627\u0644\u0627\u062a \u0627\u0644\u062a\u064a \u062a\u0645\u062a \u0645\u0631\u0627\u062c\u0639\u062a\u0647\u0627 \u0628\u0627\u0644\u0623\u0648\u0631\u0627\u0642 \u0627\u0644\u062a\u064a \u062a\u0645\u062a \u0645\u0631\u0627\u062c\u0639\u062a\u0647\u0627 \u0645\u0646 \u0642\u0628\u0644 \u0627\u0644\u0646\u0638\u0631\u0627\u0621\u060c \u0648\u0633\u062c\u0644\u0627\u062a \u0627\u0644\u062a\u062c\u0627\u0631\u0628 \u0648\u0627\u0644\u0633\u062c\u0644\u0627\u062a \u0627\u0644\u062a\u0646\u0638\u064a\u0645\u064a\u0629\u060c \u0648\u062a\u062d\u062a\u0641\u0638 \u0628\u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0641\u0627\u0631\u063a\u0629 \u0648\u0642\u064a\u0648\u062f \u0627\u0644\u062f\u0631\u0627\u0633\u0629\u060c \u0648\u062a\u0645\u064a\u0632 \u0627\u0644\u062a\u062f\u062e\u0644\u0627\u062a \u0627\u0644\u0645\u0646\u0634\u0648\u0631\u0629 \u0639\u0646 \u0645\u0648\u0627\u062f \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c. \u064a\u0645\u0643\u0646 \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0635\u064a\u0627\u063a\u0629 \u0628\u0645\u0633\u0627\u0639\u062f\u0629 AI \u0644\u0644\u0647\u064a\u0643\u0644\u061b \u062a\u0638\u0644 \u0631\u0648\u0627\u0628\u0637 \u0627\u0644\u0645\u0635\u062f\u0631 \u0648\u062d\u062f\u0648\u062f \u0627\u0644\u0623\u062f\u0644\u0629 \u0645\u0631\u0626\u064a\u0629. \u0647\u0630\u0627 \u0627\u0644\u0639\u0645\u0644 \u0644\u064a\u0633 \u0645\u0631\u0627\u062c\u0639\u0629 \u0623\u0642\u0631\u0627\u0646 \u0645\u0633\u062a\u0642\u0644\u0629 \u0623\u0648 \u0646\u0635\u064a\u062d\u0629 \u0637\u0628\u064a\u0629.","sameAs":["https:\/\/nhdbio.com"],"url":"https:\/\/nhdbio.com\/ar\/author\/qianmiao-technical-team\/"}]}},"_links":{"self":[{"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/posts\/3081","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/comments?post=3081"}],"version-history":[{"count":1,"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/posts\/3081\/revisions"}],"predecessor-version":[{"id":3087,"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/posts\/3081\/revisions\/3087"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/media\/3083"}],"wp:attachment":[{"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/media?parent=3081"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/categories?post=3081"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhdbio.com\/ar\/wp-json\/wp\/v2\/tags?post=3081"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}