{"id":3439,"date":"2026-10-05T03:29:01","date_gmt":"2026-10-05T03:29:01","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3439"},"modified":"2026-10-15T03:51:37","modified_gmt":"2026-10-15T03:51:37","slug":"%d9%85%d8%ab%d8%a7%d9%84-%d8%b9%d9%84%d9%89-%d8%af%d9%82%d8%a9-%d9%81%d8%b5%d9%84-%d8%a7%d9%84%d9%82%d9%85%d9%85-%d8%a7%d9%84%d9%85%d8%aa%d8%af%d8%a7%d8%ae%d9%84%d8%a9-%d9%81%d9%8a-%d9%83%d8%b1%d9%88","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ar\/peptide-hplc-overlapping-peaks-resolution-example\/","title":{"rendered":"\u064a\u0645\u0643\u0646 \u0644\u0642\u0645\u0629 \u0648\u0627\u062d\u062f\u0629 \u0641\u064a HPLC \u0623\u0646 \u062a\u062e\u0641\u064a \u0645\u0643\u0648\u0646\u064a\u0646: \u062a\u0639\u0644\u0645 \u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0641\u0635\u0644"},"content":{"rendered":"<p>\u064a\u062d\u062a\u0648\u064a \u0645\u062e\u0637\u0637 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0645 \u0639\u0644\u0649 \u0642\u0645\u0629 \u0648\u0627\u062d\u062f\u0629 \u0643\u0628\u064a\u0631\u0629. \u0647\u0644 \u064a\u0639\u0646\u064a \u0630\u0644\u0643 \u0623\u0646 \u0627\u0644\u0639\u064a\u0646\u0629 \u062a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0645\u0643\u0648\u0651\u0646 \u0648\u0627\u062d\u062f \u0641\u0642\u0637\u061f \u0644\u064a\u0633 \u0628\u0627\u0644\u0636\u0631\u0648\u0631\u0629. \u064a\u0645\u0643\u0646 \u0623\u0646 \u064a\u0646\u062a\u062c \u0639\u0646 \u0645\u0643\u0648\u0651\u0646\u064a\u0646 \u064a\u0635\u0644\u0627\u0646 \u0642\u0631\u064a\u0628\u064a\u0646 \u062c\u062f\u064b\u0627 \u0625\u0634\u0627\u0631\u0629 \u062a\u0628\u062f\u0648 \u0648\u0643\u0623\u0646\u0647\u0627 \u0642\u0645\u0629 \u0648\u0627\u062d\u062f\u0629. \u0625\u0644\u064a\u0643 \u0643\u064a\u0641 \u064a\u062d\u062f\u062b \u0630\u0644\u0643.<\/p>\n<h2>\u0623\u0648\u0644\u064b\u0627\u060c \u0645\u0627 \u0627\u0644\u0630\u064a \u062a\u064f\u0638\u0647\u0631\u0647 \u0627\u0644\u0642\u0645\u0629\u061f<\/h2>\n<p>\u0641\u064a HPLC\u060c \u062a\u0645\u0631\u0651 \u0627\u0644\u0639\u064a\u0646\u0629 \u0639\u0628\u0631 \u0639\u0645\u0648\u062f. \u0642\u062f \u062a\u0646\u062a\u0642\u0644 \u0627\u0644\u0645\u0643\u0648\u0651\u0646\u0627\u062a \u0627\u0644\u0645\u062e\u062a\u0644\u0641\u0629 \u0639\u0628\u0631\u0647 \u0628\u0633\u0631\u0639\u0627\u062a \u0645\u062e\u062a\u0644\u0641\u0629. \u064a\u0633\u062c\u0651\u0644 \u0627\u0644\u0643\u0627\u0634\u0641 \u0625\u0634\u0627\u0631\u0627\u062a\u0647\u0627 \u0639\u0646\u062f \u062e\u0631\u0648\u062c\u0647\u0627: \u064a\u0645\u062b\u0651\u0644 \u0627\u0644\u0645\u062d\u0648\u0631 \u0627\u0644\u0623\u0641\u0642\u064a \u0627\u0644\u0632\u0645\u0646\u060c \u0648\u064a\u0645\u062b\u0651\u0644 \u0627\u0644\u0645\u062d\u0648\u0631 \u0627\u0644\u0631\u0623\u0633\u064a \u0634\u062f\u0629 \u0627\u0644\u0625\u0634\u0627\u0631\u0629.<\/p>\n<p>\u0639\u0646\u062f\u0645\u0627 \u064a\u062e\u0631\u062c \u0645\u0643\u0648\u0651\u0646\u0627\u0646 \u0645\u0639\u064b\u0627 \u062a\u0642\u0631\u064a\u0628\u064b\u0627\u060c \u0642\u062f \u064a\u0633\u062c\u0651\u0644 \u0627\u0644\u0643\u0627\u0634\u0641 \u0634\u0643\u0644\u064b\u0627 \u0645\u062f\u0645\u062c\u064b\u0627 \u0628\u062f\u0644\u064b\u0627 \u0645\u0646 \u0642\u0645\u062a\u064a\u0646 \u0645\u0646\u0641\u0635\u0644\u062a\u064a\u0646 \u0628\u0648\u0636\u0648\u062d.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t06\/en\/1.png\" alt=\"\u0627\u0644\u0634\u0643\u0644 1. \u064a\u0645\u0643\u0646 \u0644\u0645\u0643\u0648\u0651\u0646\u064a\u0646 \u0645\u064f\u0635\u0645\u064e\u0651\u0645\u064a\u0646 \u0623\u0646 \u064a\u0646\u062a\u062c\u0627 \u0642\u0645\u0629 \u0643\u0627\u0634\u0641 \u0648\u0627\u062d\u062f\u0629 \u0645\u062f\u0645\u062c\u0629.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 1. \u0642\u062f \u062a\u0628\u062f\u0648 \u0625\u0634\u0627\u0631\u062a\u0627\u0646 \u062a\u0639\u0644\u064a\u0645\u064a\u062a\u0627\u0646 \u0645\u062a\u062f\u0627\u062e\u0644\u062a\u0627\u0646 \u0648\u0643\u0623\u0646\u0647\u0645\u0627 \u0642\u0645\u0629 \u0648\u0627\u062d\u062f\u0629.<\/figcaption><\/figure>\n<h2>\u0644\u0645\u0627\u0630\u0627 \u064a\u063a\u064a\u0651\u0631 \u0627\u0644\u0641\u0635\u0644 \u0627\u0644\u0623\u0641\u0636\u0644 \u0627\u0644\u0635\u0648\u0631\u0629<\/h2>\n<p>\u062a\u062e\u064a\u0651\u0644 \u0645\u062c\u0645\u0648\u0639\u062a\u064a\u0646 \u062a\u0645\u0631\u0651\u0627\u0646 \u0639\u0628\u0631 \u0628\u0627\u0628. \u0625\u0630\u0627 \u0648\u0635\u0644\u062a\u0627 \u0645\u0639\u064b\u0627\u060c \u0642\u062f \u062a\u0639\u064f\u062f\u0651\u0647\u0645\u0627 \u062d\u0634\u062f\u064b\u0627 \u0648\u0627\u062d\u062f\u064b\u0627. \u0648\u0625\u0630\u0627 \u0648\u0635\u0644\u062a\u0627 \u0641\u064a \u0648\u0642\u062a\u064a\u0646 \u0645\u062e\u062a\u0644\u0641\u064a\u0646\u060c \u064a\u0635\u0628\u062d \u062a\u0645\u064a\u064a\u0632 \u0627\u0644\u0645\u062c\u0645\u0648\u0639\u062a\u064a\u0646 \u0623\u0633\u0647\u0644. \u064a\u0624\u062f\u064a \u0627\u0644\u0641\u0635\u0644 \u0641\u064a \u0627\u0644\u0639\u0645\u0648\u062f \u063a\u0631\u0636\u064b\u0627 \u0645\u0634\u0627\u0628\u0647\u064b\u0627.<\/p>\n<p>\u0641\u064a \u0627\u0644\u0645\u062e\u0637\u0637 \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a \u0627\u0644\u062b\u0627\u0646\u064a\u060c \u062a\u0643\u0648\u0646 \u0627\u0644\u0642\u0645\u062a\u0627\u0646 \u0623\u0628\u0639\u062f \u0639\u0646 \u0628\u0639\u0636\u0647\u0645\u0627. \u0648\u0647\u0630\u0627 \u064a\u062c\u0639\u0644 \u0641\u062d\u0635 \u0643\u0644 \u0645\u0646\u0647\u0645\u0627 \u0648\u0642\u064a\u0627\u0633\u0647\u0627 \u0623\u0633\u0647\u0644. \u0647\u0630\u0647 \u0645\u0646\u062d\u0646\u064a\u0627\u062a \u0645\u062d\u0627\u0643\u0627\u0629\u060c \u0648\u0644\u064a\u0633\u062a \u0627\u062e\u062a\u0628\u0627\u0631\u064b\u0627 \u0642\u0628\u0644-\u0648\u0628\u0639\u062f \u0644\u062f\u0641\u0639\u0629 NHD.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t06\/en\/2.png\" alt=\"\u0627\u0644\u0634\u0643\u0644 2. \u064a\u0643\u0634\u0641 \u0627\u0644\u0641\u0635\u0644 \u0627\u0644\u0623\u0643\u0628\u0631 \u0639\u0646 \u0627\u0644\u0645\u0643\u0648\u0651\u0646\u064a\u0646 \u0627\u0644\u0645\u064f\u0635\u0645\u064e\u0651\u0645\u064a\u0646.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 2. \u0627\u0644\u0642\u0645\u0645 \u0627\u0644\u0645\u0646\u0641\u0635\u0644\u0629 \u0623\u0633\u0647\u0644 \u0641\u064a \u0627\u0644\u0641\u062d\u0635 \u0628\u0634\u0643\u0644 \u0641\u0631\u062f\u064a.<\/figcaption><\/figure>\n<h2>\u0627\u0633\u062a\u062e\u062f\u0645 \u0631\u0642\u0645\u064b\u0627 \u0644\u0648\u0635\u0641 \u0645\u0642\u062f\u0627\u0631 \u062a\u0628\u0627\u0639\u062f \u0627\u0644\u0642\u0645\u0645<\/h2>\n<p>Resolution, written Rs, compares the gap between two peak times with their widths. For this example, use widths measured at the baseline: Rs = 2 \u00d7 (second time \u2212 first time) \u00f7 (first width + second width).<\/p>\n<p>The peaks occur at 5.0 and 5.6 minutes, with baseline widths of 0.4 minutes each. Rs = 2 \u00d7 0.6 \u00f7 0.8 = 1.5. A wider gap or narrower peaks gives a larger value.<\/p>\n<p>Do not mix baseline widths with half-height widths; they use different formulas. Also, 1.5 is this example\u2019s answer, not an acceptance rule for every method.<\/p>\n<h2>\u0644\u0645\u0627\u0630\u0627 \u064a\u0647\u0645 \u0647\u0630\u0627 \u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0644\u0644\u0646\u0642\u0627\u0621<\/h2>\n<p>\u064a\u0639\u062a\u0645\u062f \u062d\u0633\u0627\u0628 \u0627\u0644\u0646\u0642\u0627\u0621 \u0627\u0644\u0642\u0627\u0626\u0645 \u0639\u0644\u0649 \u0627\u0644\u0645\u0633\u0627\u062d\u0629 \u0639\u0644\u0649 \u0627\u0644\u0625\u0634\u0627\u0631\u0627\u062a \u0627\u0644\u062a\u064a \u062a\u0641\u0635\u0644\u0647\u0627 \u0627\u0644\u0637\u0631\u064a\u0642\u0629 \u0648\u062a\u0643\u0634\u0641\u0647\u0627 \u0648\u062a\u062d\u0635\u064a\u0647\u0627. \u0625\u0630\u0627 \u0627\u062e\u062a\u0628\u0623 \u0645\u0643\u0648\u0651\u0646 \u0622\u062e\u0631 \u062a\u062d\u062a \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0647\u062f\u0641\u060c \u0641\u0642\u062f \u062a\u064f\u062f\u0631\u064e\u062c \u0625\u0634\u0627\u0631\u062a\u0647 \u0636\u0645\u0646 \u062a\u0644\u0643 \u0627\u0644\u0645\u0633\u0627\u062d\u0629.<\/p>\n<p>\u0644\u0630\u0644\u0643 \u0627\u0642\u0631\u0623 \u0627\u0644\u0645\u062e\u0637\u0637 \u0627\u0644\u0644\u0648\u0646\u064a \u0643\u0627\u0645\u0644\u064b\u0627 \u0648\u0627\u0644\u0637\u0631\u064a\u0642\u0629\u060c \u0644\u0627 \u0627\u0644\u0646\u0633\u0628\u0629 \u0627\u0644\u0645\u0626\u0648\u064a\u0629 \u0641\u0642\u0637. \u0643\u0645\u0627 \u0623\u0646 \u0634\u0643\u0644 \u0627\u0644\u0642\u0645\u0629 \u0648\u062d\u062f\u0647 \u0644\u0627 \u064a\u0645\u0643\u0646\u0647 \u062a\u062d\u062f\u064a\u062f \u0647\u0648\u064a\u0629 \u0643\u0644 \u0645\u0643\u0648\u0651\u0646.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t06\/en\/3.png\" alt=\"\u0627\u0644\u0634\u0643\u0644 3. \u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0644\u0639\u0631\u0648\u0636 \u062e\u0637 \u0627\u0644\u0623\u0633\u0627\u0633 0.4 \u062f\u0642\u064a\u0642\u0629 \u0648\u0627\u0644\u0623\u0632\u0645\u0646\u0629 5.0 \u06485.6 \u062f\u0642\u064a\u0642\u0629\u060c \u062a\u0643\u0648\u0646 \u062f\u0642\u0629 \u0627\u0644\u0641\u0635\u0644 \u0627\u0644\u062a\u0639\u0644\u064a\u0645\u064a\u0629 1.5.\"><figcaption>\u0627\u0644\u0634\u0643\u0644 3. \u0641\u0635\u0644 \u0627\u0644\u0642\u0645\u0645 \u0648\u0627\u062e\u062a\u064a\u0627\u0631 \u0627\u0644\u0645\u0633\u0627\u062d\u0629 \u064a\u0624\u062b\u0631\u0627\u0646 \u0639\u0644\u0649 \u0627\u0644\u062a\u0641\u0633\u064a\u0631.<\/figcaption><\/figure>\n<h2>\u062c\u0631\u0651\u0628 \u0632\u0648\u062c\u064b\u0627 \u0623\u0642\u0631\u0628 \u0645\u0646 \u0627\u0644\u0642\u0645\u0645<\/h2>\n<p>\u0623\u0628\u0642\u0650 \u0639\u0631\u0636\u064a \u062e\u0637 \u0627\u0644\u0623\u0633\u0627\u0633 \u0639\u0646\u062f 0.4 \u062f\u0642\u064a\u0642\u0629\u060c \u0644\u0643\u0646 \u062d\u0631\u0651\u0643 \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u062b\u0627\u0646\u064a\u0629 \u0625\u0644\u0649 5.2 \u062f\u0642\u064a\u0642\u0629. \u0627\u0644\u0641\u062c\u0648\u0629 \u0627\u0644\u0622\u0646 0.2 \u062f\u0642\u064a\u0642\u0629 \u0641\u0642\u0637.<\/p>\n<div class=\"exercise\"><strong>\u0645\u0627 \u0642\u064a\u0645\u0629 Rs \u0644\u0644\u0642\u0645\u0645 \u0639\u0646\u062f 5.0 \u0648 5.2 \u062f\u0642\u064a\u0642\u0629\u061f<\/strong><\/p>\n<details>\n<summary>\u0639\u0631\u0636 \u0627\u0644\u0625\u062c\u0627\u0628\u0629<\/summary>\n<p>2 \u00d7 0.2 \u00f7 0.8 = 0.5. The smaller value reflects a smaller gap relative to peak width.<\/p>\n<\/details>\n<\/div>\n<h2>\u0642\u0631\u0627\u0621\u0629 \u0645\u0639\u0644\u0648\u0645\u0627\u062a \u062a\u062d\u0644\u064a\u0644 GHK-Cu \u0623\u0648 KPV<\/h2>\n<p>\u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0644\u0640 GHK-Cu \u0623\u0648 KPV\u060c \u0627\u0633\u0623\u0644 \u0639\u0646 \u0633\u062c\u0644\u0627\u062a \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0645\u062a\u0627\u062d\u0629 \u0648\u0643\u064a\u0641 \u062a\u0645 \u062a\u062d\u062f\u064a\u062f \u0627\u0644\u0625\u0634\u0627\u0631\u0629 \u0627\u0644\u0647\u062f\u0641. \u0625\u0630\u0627 \u0642\u064f\u062f\u0650\u0651\u0645 \u0631\u0642\u0645 \u0646\u0642\u0627\u0621\u060c \u0627\u0642\u0631\u0623\u0647 \u0645\u0639 \u0637\u0631\u064a\u0642\u062a\u0647 \u0648\u0645\u062e\u0637\u0637\u0647 \u0627\u0644\u0643\u0627\u0645\u0644.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/ghk-cu\/\">GHK-Cu<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d9%85%d9%86%d8%aa%d8%ac%d8%a7%d8%aa\/kpv\/\">KPV<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%82%d8%aa%d8%a8%d8%a7%d8%b3\/\">\u0646\u0627\u0642\u0634 \u0645\u062a\u0637\u0644\u0628\u0627\u062a \u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0645\u0633\u062a\u0646\u062f\u0627\u062a \u0645\u0639 NHD<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ar\/%d8%a7%d9%84%d8%b7%d8%b1%d9%82-%d8%a7%d9%84%d8%aa%d8%ad%d9%84%d9%8a%d9%84%d9%8a%d8%a9\/%d8%aa%d9%81%d8%b3%d9%8a%d8%b1-%d9%85%d8%ae%d8%b7%d8%b7-%d8%a7%d9%84%d9%83%d8%b1%d9%88%d9%85%d8%a7%d8%aa%d9%88%d8%ba%d8%b1%d8%a7%d9%85\/\">\u062a\u0627\u0628\u0639 \u0645\u0639 \u0627\u0644\u062f\u0644\u064a\u0644 \u0627\u0644\u062a\u062d\u0644\u064a\u0644\u064a<\/a><\/p>\n<footer><strong>\u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0648\u0645\u0644\u0627\u062d\u0638\u0627\u062a \u0627\u0644\u0623\u0645\u062b\u0644\u0629<\/strong><\/p>\n<p>\u0627\u0644\u0623\u0631\u0642\u0627\u0645 \u0648\u0627\u0644\u0645\u0646\u062d\u0646\u064a\u0627\u062a \u0623\u0645\u062b\u0644\u0629 \u062a\u0639\u0644\u064a\u0645\u064a\u0629\u060c \u0648\u0644\u064a\u0633\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 \u062f\u0641\u0639\u0629 \u0645\u0646\u062a\u062c. \u062a\u0645 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0627\u0644\u0645\u0635\u0627\u062f\u0631 \u0641\u064a 11 \u0623\u0643\u062a\u0648\u0628\u0631 2026.<\/p>\n<ul>\n<li><a href=\"https:\/\/www.agilent.com\/about\/data-integrity\/en\/5-tips-chromatographic-integration.html\">Agilent \u00b7 chromatographic integration<\/a><\/li>\n<li><a href=\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\">ICH Q2(R2) \u00b7 analytical validation<\/a><\/li>\n<\/ul>\n<p>\u0627\u0644\u0643\u062a\u0627\u0628\u0629 \u0628\u0645\u0633\u0627\u0639\u062f\u0629 \u0627\u0644\u0630\u0643\u0627\u0621 \u0627\u0644\u0627\u0635\u0637\u0646\u0627\u0639\u064a \u0648\u0645\u0631\u0627\u062c\u0639\u0629 \u0627\u0644\u0645\u0635\u0627\u062f\u0631. \u0645\u0648\u0627\u062f \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c \u0645\u062e\u0635\u0635\u0629 \u0644\u0644\u0628\u062d\u062b \u0648\u0627\u0644\u062a\u0637\u0648\u064a\u0631 \u0648\u0627\u0644\u062a\u0635\u0646\u064a\u0639\u060c \u0648\u0644\u064a\u0633 \u0644\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0634\u0631\u064a \u0623\u0648 \u0627\u0644\u0628\u064a\u0637\u0631\u064a.<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u062a\u0639\u0631\u0651\u0641 \u0639\u0644\u0649 \u0633\u0628\u0628 \u0623\u0647\u0645\u064a\u0629 \u0634\u0643\u0644 \u0627\u0644\u0642\u0645\u0629 \u0642\u0628\u0644 \u0623\u0646 \u062a\u0642\u0631\u0623 \u0646\u0633\u0628\u0629 \u0627\u0644\u0646\u0642\u0627\u0621.<\/p>","protected":false},"author":1,"featured_media":3965,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"329,348,352,318","pim_article_type":"Practical research guide","pim_evidence_level":"Technical guidance with labeled teaching examples","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Study results and labeled examples are separated. Original-source, arithmetic, readability and internal-link checks; no independent human peer review recorded.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"AI-assisted writing and source checks; conceptual illustrated cover.","pim_sources":"[{\"title\":\"ICH Q2(R2): Validation of Analytical Procedures\",\"note\":\"Defines validation characteristics and distinguishes quantitative analytical purposes.\",\"url\":\"https:\/\/www.fda.gov\/regulatory-information\/search-fda-guidance-documents\/q2r2-validation-analytical-procedures\"},{\"title\":\"Agilent: Five Tips for Chromatographic Integration\",\"note\":\"Agilent: Five Tips for Chromatographic Integration. Accessed 2026-10-05.\",\"url\":\"https:\/\/www.agilent.com\/about\/data-integrity\/en\/5-tips-chromatographic-integration.html\"},{\"title\":\"Teaching reference \u2014 database.ich.org \u2014 ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\",\"note\":\"\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\"},{\"title\":\"Peptide Isolation \u2013 Method Development Considerations\",\"note\":\"Peptide Isolation \u2013 Method Development Considerations\",\"url\":\"https:\/\/www.waters.com\/nextgen\/gb\/en\/education\/primers\/practical-approaches-to-peptide-isolation\/method-development-considerations.html\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3439","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>One HPLC Peak Can Hide Two Components: Learn to Read Separation | NHD<\/title>\n<meta name=\"description\" content=\"Learn why peak shape matters before you read a purity percentage.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ar\/\u0645\u062b\u0627\u0644-\u0639\u0644\u0649-\u062f\u0642\u0629-\u0641\u0635\u0644-\u0627\u0644\u0642\u0645\u0645-\u0627\u0644\u0645\u062a\u062f\u0627\u062e\u0644\u0629-\u0641\u064a-\u0643\u0631\u0648\/\" \/>\n<meta property=\"og:locale\" content=\"ar_AR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"One HPLC Peak Can Hide Two Components: Learn to Read Separation\" \/>\n<meta property=\"og:description\" content=\"Learn why peak shape matters before you read a purity percentage.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nhdbio.com\/ar\/\u0645\u062b\u0627\u0644-\u0639\u0644\u0649-\u062f\u0642\u0629-\u0641\u0635\u0644-\u0627\u0644\u0642\u0645\u0645-\u0627\u0644\u0645\u062a\u062f\u0627\u062e\u0644\u0629-\u0641\u064a-\u0643\u0631\u0648\/\" \/>\n<meta property=\"og:site_name\" content=\"NHD\" \/>\n<meta property=\"article:published_time\" content=\"2026-10-05T03:29:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-10-15T03:51:37+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/nhd-comic-3439.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"800\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"NHD Technical Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"twitter:label1\" content=\"\u0643\u064f\u062a\u0628 \u0628\u0648\u0627\u0633\u0637\u0629\" \/>\n\t<meta name=\"twitter:data1\" content=\"NHD Technical Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u0648\u0642\u062a \u0627\u0644\u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0645\u064f\u0642\u062f\u0651\u0631\" \/>\n\t<meta name=\"twitter:data2\" content=\"\u062f\u0642\u064a\u0642\u062a\u0627\u0646\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-hplc-overlapping-peaks-resolution-example\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-hplc-overlapping-peaks-resolution-example\\\/\"},\"author\":{\"name\":\"NHD Technical Team\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/person\\\/0ffb0dec7fda0f720073fc468fac6fc1\"},\"headline\":\"One HPLC Peak Can Hide Two Components: Learn to Read Separation\",\"datePublished\":\"2026-10-05T03:29:01+00:00\",\"dateModified\":\"2026-10-15T03:51:37+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-hplc-overlapping-peaks-resolution-example\\\/\"},\"wordCount\":505,\"publisher\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-hplc-overlapping-peaks-resolution-example\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/nhd-comic-3439.jpg\",\"articleSection\":[\"Evidence &amp; 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