{"id":3683,"date":"2026-10-09T08:34:54","date_gmt":"2026-10-09T08:34:54","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3683"},"modified":"2026-10-10T07:26:41","modified_gmt":"2026-10-10T07:26:41","slug":"%d8%aa%d8%a3%d8%ab%d9%8a%d8%b1%d8%a7%d8%aa-%d8%ad%d9%88%d8%a7%d9%81-%d8%b5%d9%81%d9%8a%d8%ad%d8%a9-%d9%81%d8%ad%d8%b5-%d8%a7%d9%84%d8%a8%d8%a8%d8%aa%d9%8a%d8%af","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ar\/peptide-assay-plate-edge-effects\/","title":{"rendered":"\u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u0628\u0628\u062a\u064a\u062f: \u0647\u0644 \u064a\u0645\u0643\u0646 \u0623\u0646 \u064a\u063a\u064a\u0631 \u0645\u0648\u0636\u0639 \u0627\u0644\u0635\u0641\u064a\u062d\u0629 \u0646\u062a\u064a\u062c\u062a\u0643\u061f"},"content":{"rendered":"

\u0644\u0646\u0641\u062a\u0631\u0636 \u0623\u0646 \u0623\u0642\u0648\u0649 \u0625\u0634\u0627\u0631\u0629 \u0641\u064a \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u062a\u0638\u0647\u0631 \u0639\u0644\u0649 \u0637\u0648\u0644 \u062d\u0627\u0641\u0629 \u0627\u0644\u0637\u0628\u0642. \u0642\u0628\u0644 \u0623\u0646 \u062a\u0642\u0631\u0631 \u0623\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0647\u0648 \u0633\u0628\u0628 \u0627\u0644\u062a\u063a\u064a\u064a\u0631\u060c \u062a\u062d\u0642\u0642 \u0645\u0646 \u0645\u0643\u0627\u0646 \u0648\u0636\u0639 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0627\u062a \u0648\u0627\u0644\u0634\u0648\u0627\u0647\u062f. \u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u0630\u064a \u064a\u062a\u0628\u0639 \u0645\u0648\u0636\u0639 \u0627\u0644\u0637\u0628\u0642 \u064a\u0633\u062a\u062d\u0642 \u062a\u062d\u0642\u064a\u0642\u064b\u0627 \u0645\u062e\u062a\u0644\u0641\u064b\u0627 \u0639\u0646 \u0627\u0644\u0646\u0645\u0637 \u0627\u0644\u0630\u064a \u064a\u062a\u0628\u0639 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629.<\/p>\n

\u0627\u0644\u0637\u0628\u0642 \u062c\u0632\u0621 \u0645\u0646 \u0627\u0644\u0642\u064a\u0627\u0633<\/h2>\n

A 1980 enzyme-immunoassay study examined protein adsorption on microtitre plates. It reported adsorption coefficients of variation of 5.2\u201329.5% and higher adsorption in edge wells. In one sandwich assay, the reported variation for an individual sample was \u00b118%. These findings concerned the tested immunoassays and plate systems, not LL-37, MOTS-c or NHD material. They show why a well should not be assumed interchangeable with every other well. \u0627\u0642\u0631\u0623 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0623\u0635\u0644\u064a\u0629\u060c PMID 6154544<\/a>.<\/p>\n

\u0643\u0645 \u0639\u062f\u062f \u0627\u0644\u0622\u0628\u0627\u0631 \u0639\u0644\u0649 \u0627\u0644\u062d\u0627\u0641\u0629\u061f<\/h2>\n

Consider a rectangular 96-well plate with 8 rows and 12 columns. Its outer border contains 2 \u00d7 12 + 2 \u00d7 (8 \u2212 2) = 36 wells. The interior contains 6 \u00d7 10 = 60 wells. Thus 36 \u00f7 96 = 37.5% of the wells lie on the border.<\/p>\n\n\n\n\n\n\n\n
\u0627\u0644\u0645\u0646\u0637\u0642\u0629<\/th>\n\u0627\u0644\u0622\u0628\u0627\u0631<\/th>\n\u0627\u0644\u0645\u0639\u0646\u0649<\/th>\n<\/tr>\n<\/thead>\n
\u0627\u0644\u062d\u062f \u0627\u0644\u062e\u0627\u0631\u062c\u064a<\/td>\n36<\/td>\n\u0627\u0644\u0645\u0648\u0627\u0636\u0639 \u0627\u0644\u062a\u064a \u064a\u062c\u0628 \u0641\u062d\u0635\u0647\u0627 \u0628\u062d\u062b\u064b\u0627 \u0639\u0646 \u0646\u0645\u0637 \u0645\u062a\u0639\u0644\u0642 \u0628\u0627\u0644\u0645\u0648\u0642\u0639<\/td>\n<\/tr>\n
\u0627\u0644\u062f\u0627\u062e\u0644\u064a\u0629<\/td>\n60<\/td>\n\u0645\u062c\u0645\u0648\u0639\u0629 \u0645\u062e\u062a\u0644\u0641\u0629 \u0645\u0646 \u0627\u0644\u0645\u0648\u0627\u0636\u0639\u060c \u0648\u0644\u064a\u0633\u062a \u062a\u0644\u0642\u0627\u0626\u064a\u064b\u0627 \u0636\u0627\u0628\u0637\u064b\u0627 \u063a\u064a\u0631 \u0645\u062a\u0623\u062b\u0631<\/td>\n<\/tr>\n
\u0627\u0644\u0625\u062c\u0645\u0627\u0644\u064a<\/td>\n96<\/td>\n\u0647\u0646\u062f\u0633\u064a \u0641\u0642\u0637\u061b \u0648\u0644\u0627 \u064a\u064f\u0633\u062a\u062f\u0644 \u0645\u0646\u0647 \u0639\u0644\u0649 \u062d\u062c\u0645 \u062a\u0623\u062b\u064a\u0631 \u062a\u062c\u0631\u064a\u0628\u064a<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u0647\u0630\u0627 \u062d\u0633\u0627\u0628 \u0647\u0646\u062f\u0633\u064a. \u0648\u0647\u0648 \u0644\u0627 \u064a\u0639\u0646\u064a \u0623\u0646 37.5% \u0645\u0646 \u0627\u0644\u0642\u064a\u0627\u0633\u0627\u062a \u062e\u0627\u0637\u0626\u0629\u060c \u0623\u0648 \u0623\u0646\u0647 \u064a\u0646\u0628\u063a\u064a \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u062c\u0645\u064a\u0639 \u0627\u0644\u0622\u0628\u0627\u0631 \u0627\u0644\u062d\u062f\u0648\u062f\u064a\u0629.<\/p>\n

\u0627\u062d\u062a\u0641\u0638 \u0628\u062e\u0631\u064a\u0637\u0629 \u0627\u0644\u0635\u0641\u064a\u062d\u0629<\/h2>\n

\u0633\u062c\u0651\u0644 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0648\u0627\u0644\u0636\u0627\u0628\u0637 \u0648\u0645\u0639\u0631\u0651\u0641 \u0627\u0644\u0639\u064a\u0646\u0629 \u0648\u0645\u0648\u0636\u0639 \u0643\u0644 \u0628\u0626\u0631. \u0648\u0625\u0630\u0627 \u0643\u0627\u0646\u062a \u0645\u0639\u0627\u0644\u062c\u0629 \u0648\u0627\u062d\u062f\u0629 \u062a\u0634\u063a\u0644 \u0627\u0644\u0622\u0628\u0627\u0631 \u0627\u0644\u062d\u062f\u0648\u062f\u064a\u0629 \u0641\u0642\u0637 \u0628\u064a\u0646\u0645\u0627 \u064a\u0634\u063a\u0644 \u0627\u0644\u0636\u0627\u0628\u0637 \u0627\u0644\u0622\u0628\u0627\u0631 \u0627\u0644\u0645\u0631\u0643\u0632\u064a\u0629 \u0641\u0642\u0637\u060c \u0641\u0642\u062f \u062c\u0639\u0644 \u0627\u0644\u062a\u0635\u0645\u064a\u0645 \u0641\u0635\u0644 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0639\u0646 \u0627\u0644\u0645\u0648\u0642\u0639 \u0623\u0645\u0631\u064b\u0627 \u0635\u0639\u0628\u064b\u0627. \u0648\u0644\u0627 \u064a\u0645\u0643\u0646 \u0644\u0646\u062a\u064a\u062c\u0629 \u0648\u062d\u062f\u0647\u0627 \u0623\u0646 \u062a\u0641\u0635\u0644 \u0628\u064a\u0646\u0647\u0645\u0627.<\/p>\n

\u0623\u062b\u0646\u0627\u0621 \u062a\u0637\u0648\u064a\u0631 \u0627\u0644\u0637\u0631\u064a\u0642\u0629\u060c \u0642\u0627\u0631\u0646 \u0638\u0631\u0648\u0641 \u0627\u0644\u062a\u062d\u0643\u0645 \u0627\u0644\u0645\u0643\u0627\u0641\u0626\u0629 \u0639\u0628\u0631 \u0627\u0644\u0645\u0648\u0627\u0636\u0639 \u0627\u0644\u062a\u064a \u062a\u062e\u0637\u0637 \u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645\u0647\u0627. \u064a\u0645\u0643\u0646 \u0623\u0646 \u064a\u062f\u0641\u0639 \u0646\u0645\u0637 \u0627\u0644\u0645\u0648\u0636\u0639 \u0625\u0644\u0649 \u0625\u062c\u0631\u0627\u0621 \u0641\u062d\u0648\u0635\u0627\u062a \u0644\u0633\u0637\u062d \u0627\u0644\u0644\u0648\u062d\u0629 \u0648\u0627\u0644\u0645\u0646\u0627\u0648\u0644\u0629 \u0648\u0627\u0644\u0638\u0631\u0648\u0641 \u0627\u0644\u0628\u064a\u0626\u064a\u0629. \u064a\u0639\u062a\u0645\u062f \u0627\u0644\u0641\u062d\u0635 \u0627\u0644\u0645\u0646\u0627\u0633\u0628 \u0639\u0644\u0649 \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629. \u0644\u0627 \u064a\u0644\u0632\u0645 \u0623\u0646 \u064a\u0643\u0648\u0646 \u0644\u0641\u062d\u0635 \u0627\u0631\u062a\u0628\u0627\u0637 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0648\u0641\u062d\u0635 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062d\u064a\u0629 \u0627\u0644\u0633\u0628\u0628 \u0646\u0641\u0633\u0647 \u0641\u064a \u0627\u0644\u062a\u0628\u0627\u064a\u0646.<\/p>\n

\u062d\u062f\u062f \u0627\u0644\u0627\u0633\u062a\u062b\u0646\u0627\u0621\u0627\u062a \u0642\u0628\u0644 \u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0646\u062a\u064a\u062c\u0629<\/h2>\n

Do not remove a border result simply because it disagrees with the desired conclusion. Use the assay’s planned quality criteria and document the reason for any excluded measurement. If the experiment cannot distinguish treatment from position, repeating it with a suitable design may be more informative than forcing a strong claim from the existing data.<\/p>\n

\u0627\u0631\u0628\u0637 \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0628\u0627\u0644\u0645\u0627\u062f\u0629<\/h2>\n

\u0639\u0646\u062f \u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0627\u062f\u0629 \u0628\u062d\u062b\u064a\u0629 LL-37<\/a> \u0623\u0648 \u0645\u0627\u062f\u0629 \u0628\u062d\u062b\u064a\u0629 MOTS-c<\/a>\u060c \u0627\u062d\u062a\u0641\u0638 \u0628\u0627\u0644\u0647\u0648\u064a\u0629 \u0627\u0644\u0645\u0639\u0644\u0646\u0629 \u0648\u0627\u0644\u0634\u0643\u0644 \u0627\u0644\u0645\u0648\u0631\u0651\u062f \u0648\u0623\u0633\u0627\u0633 \u0627\u0644\u0643\u0645\u064a\u0629 \u0648\u0633\u062c\u0644\u0627\u062a \u0627\u0644\u062f\u0641\u0639\u0627\u062a \u0627\u0644\u0645\u062a\u0627\u062d\u0629 \u0645\u0639 \u062e\u0631\u064a\u0637\u0629 \u0627\u0644\u0644\u0648\u062d\u0629. \u064a\u0645\u0643\u0646 \u0623\u0646 \u062a\u0623\u062a\u064a \u0627\u0644\u0625\u0634\u0627\u0631\u0629 \u0627\u0644\u0645\u062a\u063a\u064a\u0631\u0629 \u0645\u0646 \u0627\u0644\u0645\u0627\u062f\u0629 \u0623\u0648 \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0623\u0648 \u062a\u0641\u0627\u0639\u0644\u0647\u0645\u0627\u061b \u062a\u0633\u0627\u0639\u062f \u0641\u062d\u0648\u0635\u0627\u062a \u0627\u0644\u0645\u0648\u0642\u0639 \u0639\u0644\u0649 \u062a\u062c\u0646\u0628 \u062a\u0641\u0633\u064a\u0631 \u0645\u062a\u0633\u0631\u0639.<\/p>\n

\u0627\u0633\u0623\u0644 \u0639\u0646 \u0646\u0633\u062e\u0629 \u0627\u0644\u0628\u062d\u062b \u0627\u0644\u0645\u062e\u062a\u0627\u0631\u0629<\/a> \u0648\u0639\u0646 \u0627\u0644\u0633\u062c\u0644\u0627\u062a \u0627\u0644\u062a\u064a \u064a\u062a\u0637\u0644\u0628\u0647\u0627 \u0645\u0634\u0631\u0648\u0639\u0643. \u0644\u0627 \u062a\u064f\u062b\u0628\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0641\u062d\u0648\u0635 \u0627\u0644\u0645\u0646\u0634\u0648\u0631\u0629 \u062e\u0635\u0627\u0626\u0635 \u062f\u0641\u0639\u0629 NHD. \u0645\u0648\u0627\u062f \u0627\u0644\u0643\u062a\u0627\u0644\u0648\u062c \u0645\u062e\u0635\u0635\u0629 \u0644\u0644\u0628\u062d\u062b \u0648\u0627\u0644\u062a\u0637\u0648\u064a\u0631 \u0648\u0627\u0644\u062a\u0635\u0646\u064a\u0639\u060c \u0648\u0644\u064a\u0633 \u0644\u0644\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0634\u0631\u064a \u0623\u0648 \u0627\u0644\u0628\u064a\u0637\u0631\u064a.<\/p>","protected":false},"excerpt":{"rendered":"

A plate’s outer wells can behave differently from its center. Use a clear plate map and comparable controls before attributing a signal change to the peptide.<\/p>","protected":false},"author":1,"featured_media":3932,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"348,352","pim_article_type":"Research methods explained","pim_evidence_level":"Authoritative guidance and explicitly labeled planning examples","pim_reviewer":"Codex AI editorial","pim_reviewed_date":"2026-10-09","pim_editorial_note":"AI-assisted source and readability checks, not independent peer review.","pim_review_status":"reviewed","pim_review_reason":"Source attribution, illustrative arithmetic, product relevance and plain-language checks.","pim_creation_method":"AI-assisted writing","pim_sources":"[]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3683","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"\nPeptide Assays: Could the Plate Position Change Your Result?<\/title>\n<meta name=\"description\" content=\"A plate's outer wells can behave differently from its center. 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