{"id":1163,"date":"2026-09-04T05:56:46","date_gmt":"2026-09-04T05:56:46","guid":{"rendered":"https:\/\/nhdbio.com\/research-tools\/peptide-sequence-analyzer\/"},"modified":"2026-10-09T08:22:35","modified_gmt":"2026-10-09T08:22:35","slug":"%e8%82%bd%e5%ba%8f%e5%88%97%e5%88%86%e6%9e%90%e4%bb%aa","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/cn\/research-tools\/peptide-sequence-analyzer\/","title":{"rendered":"\u80bd\u5e8f\u5217\u5206\u6790\u4eea"},"content":{"rendered":"<p>\u8ba1\u7b97\u80bd\u7ec4\u6210\u3001\u5206\u5b50\u5f0f\u3001\u5e73\u5747\u548c\u5355\u540c\u4f4d\u7d20\u8d28\u91cf\u3001\u7406\u8bba pI\u3001\u4f30\u8ba1\u7535\u8377\u3001\u4eb2\u6c34\u6027\u548c A280 \u6d88\u5149\u503c\u3002<\/p>\n<section id=\"nhd-reading-paths-20261009\">\n<h2>Before relying on a sequence calculation<\/h2>\n<p>\u5e8f\u5217\u8ba1\u7b97\u63cf\u8ff0\u7684\u662f\u60a8\u8f93\u5165\u7684\u5047\u8bbe\uff0c\u800c\u4e0d\u662f\u67d0\u4e2a\u5b9e\u9645\u6837\u54c1\u7684\u8eab\u4efd\u3002\u8bf7\u5c06\u5e8f\u5217\u3001\u672b\u7aef\u57fa\u56e2\u3001\u4fee\u9970\u548c\u8d28\u91cf\u7ea6\u5b9a\u4e0e\u7ed3\u679c\u4e00\u8d77\u8bb0\u5f55\u3002<\/p>\n<div class=\"nhd-reading-paths\">\n<article>\n<h3>Check modifications<\/h3>\n<p>A lipid tail or another modification can change the expected mass.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/cn\/%e5%8d%a1%e6%a0%bc%e7%91%9e%e6%9e%97%e8%82%bd%e5%ba%8f%e5%88%97%e8%ae%a1%e7%ae%97%e5%99%a8%e8%84%82%e5%8c%96%e7%bb%93%e6%9e%84\/\">\u9605\u8bfb\u6307\u5357<\/a><\/p>\n<\/article>\n<article>\n<h3>Check unusual residues<\/h3>\n<p>A simple letter sequence may leave out nonstandard chemistry.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/cn\/ss31-%e9%9d%9e%e6%a0%87%e5%87%86%e6%b0%a8%e5%9f%ba%e9%85%b8%e5%ba%8f%e5%88%97%e8%ae%a1%e7%ae%97%e5%99%a8\/\">\u9605\u8bfb\u6307\u5357<\/a><\/p>\n<\/article>\n<article>\n<h3>Compare with measured evidence<\/h3>\n<p>The expected mass and the measured charge-state signal are different values.<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/cn\/%e5%a4%9a%e8%82%bdlc-ms%e7%94%b5%e8%8d%b7%e6%80%81m-z%e7%ae%80%e5%8d%95%e7%a4%ba%e4%be%8b\/\">\u9605\u8bfb\u6307\u5357<\/a><\/p>\n<\/article>\n<\/div>\n<p><a href=\"https:\/\/nhdbio.com\/cn\/%e4%ba%a7%e5%93%81\/\">\u6d4f\u89c8\u7814\u7a76\u8d44\u6599<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/cn\/%e5%bc%95%e7%94%a8\/\">\u54a8\u8be2\u4ea7\u54c1\u6216\u5e8f\u5217<\/a><\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"<p>\u5e8f\u5217\u8ba1\u7b97\u63cf\u8ff0\u7684\u662f\u60a8\u8f93\u5165\u7684\u5047\u8bbe\uff0c\u800c\u4e0d\u662f\u67d0\u4e2a\u5b9e\u9645\u6837\u54c1\u7684\u8eab\u4efd\u3002\u8bf7\u5c06\u5e8f\u5217\u3001\u672b\u7aef\u57fa\u56e2\u3001\u4fee\u9970\u548c\u8d28\u91cf\u7ea6\u5b9a\u4e0e\u7ed3\u679c\u4e00\u8d77\u8bb0\u5f55\u3002<\/p>","protected":false},"author":0,"featured_media":0,"parent":704,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1163","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Peptide Sequence Analyzer | Research Knowledge Center<\/title>\n<meta name=\"description\" content=\"A sequence calculation describes the assumptions you enter, not the identity of a physical sample. 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