{"id":3104,"date":"2026-10-04T13:31:25","date_gmt":"2026-10-04T13:31:25","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3104"},"modified":"2026-10-04T15:11:42","modified_gmt":"2026-10-04T15:11:42","slug":"%e8%82%bd%e4%ba%8c%e7%a1%ab%e9%94%ae%e4%b8%a4%e4%b8%aa%e6%b0%a2%e7%9a%84%e8%b4%a8%e9%87%8f%e5%b7%ae","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/cn\/peptide-disulfide-bond-two-hydrogen-mass-difference\/","title":{"rendered":"\u4e8c\u786b\u952e\u4f1a\u4f7f\u80bd\u8d28\u91cf\u53d8\u5316\u7ea6 2 Da\u3002\u4e3a\u4ec0\u4e48\uff1f"},"content":{"rendered":"<p>\u540c\u4e00\u80bd\u7684\u4e24\u4e2a\u7248\u672c\u53ef\u4ee5\u5177\u6709\u76f8\u540c\u7684\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u4f46\u7531\u4e8e\u5206\u5b50\u5185\u5f62\u6210\u4e86\u952e\u800c\u8d28\u91cf\u7565\u6709\u4e0d\u540c\u3002\u4e8c\u786b\u952e\u662f\u4e00\u4e2a\u4f8b\u5b50\uff1a\u5728\u7b80\u5355\u6c27\u5316\u6a21\u578b\u4e0b\uff0c\u5f62\u6210\u4e00\u4e2a\u952e\u4f1a\u79fb\u9664\u4e24\u4e2a\u6c22\u539f\u5b50\u3002<\/p>\n<h2>\u952e\u5f62\u6210\u65f6\u4f1a\u53d1\u751f\u4ec0\u4e48\uff1f<\/h2>\n<p>\u4e24\u4e2a\u534a\u80f1\u6c28\u9178\u4fa7\u94fe\u53ef\u4ee5\u901a\u8fc7\u5176\u786b\u539f\u5b50\u8fde\u63a5\u3002\u4e0e\u76f8\u5e94\u7684\u8fd8\u539f\u786b\u9187\u76f8\u6bd4\uff0c\u751f\u6210\u7684\u4e8c\u786b\u5316\u7269\u5c11\u4e24\u4e2a\u6c22\u539f\u5b50\u3002\u6b64\u89e3\u91ca\u5047\u8bbe\u6307\u5b9a\u7684\u952e\u5f62\u6210\u4e14\u65e0\u5176\u4ed6\u5316\u5b66\u53d8\u5316\uff1b\u5b9e\u9645\u6837\u54c1\u53ef\u80fd\u542b\u6709\u6df7\u5408\u7269\u6216\u989d\u5916\u4fee\u9970\u3002<\/p>\n<h2>\u8ba1\u7b97\u5f97\u51fa\u7684\u5dee\u503c\u7ea6\u4e3a 2.016 Da<\/h2>\n<p>NIST \u7ed9\u51fa\u6c22-1 \u539f\u5b50\u7684\u8d28\u91cf\u4e3a 1.00782503223 Da\u3002\u79fb\u9664\u4e24\u4e2a\u6c22\u539f\u5b50\u540e\uff0c\u8ba1\u7b97\u5f97\u5230\u7684\u4e2d\u6027\u5355\u540c\u4f4d\u7d20\u8d28\u91cf\u53d8\u5316\u4e3a \u22122.01565006446 Da\u3002\u5728\u4e00\u4e2a\u6559\u5b66\u793a\u4f8b\u4e2d\uff0c\u4e2d\u6027\u5355\u540c\u4f4d\u7d20\u8d28\u91cf\u4e3a 2000.00000 Da \u7684\u8fd8\u539f\u80bd\u5728\u5f62\u6210\u4e00\u4e2a\u8fd9\u6837\u7684\u952e\u540e\u5927\u7ea6\u53d8\u4e3a 1997.98435 Da\u3002\u8fd9\u4e9b\u662f\u8bf4\u660e\u6027\u6570\u5b57\uff0c\u4e0d\u662f\u5b9e\u6d4b\u6279\u6b21\u3002<\/p>\n<h2>\u539f\u59cb\u8c31\u56fe\u53ef\u80fd\u663e\u793a\u8f83\u5c0f\u7684\u95f4\u9694<\/h2>\n<p>\u5982\u679c\u4e24\u4e2a\u79bb\u5b50\u7684\u7535\u8377\u5747\u4e3a +2\uff0c\u5219\u76f8\u5e94\u7684 m\/z \u95f4\u9694\u7ea6\u4e3a 1.007825\u3002\u8fd9\u662f\u4e2d\u6027\u8d28\u91cf\u5dee\u7684\u4e00\u534a\u3002\u56e0\u6b64\uff0c\u5373\u4f7f\u7406\u89e3\u4e86\u5316\u5b66\u539f\u7406\uff0c\u76f4\u63a5\u5c06\u4e2d\u6027\u8ba1\u7b97\u8d28\u91cf\u4e0e\u672a\u6307\u6d3e\u7684\u539f\u59cb\u79bb\u5b50\u5cf0\u8fdb\u884c\u6bd4\u8f83\u4e5f\u53ef\u80fd\u9020\u6210\u6df7\u6dc6\u3002<\/p>\n<h2>\u8d28\u91cf\u4e0d\u80fd\u8bc1\u660e\u952e\u7684\u8fde\u63a5\u65b9\u5f0f<\/h2>\n<p>\u5f53\u5b58\u5728\u591a\u4e2a\u534a\u80f1\u6c28\u9178\u65f6\uff0c\u4e0e\u4e00\u4e2a\u4e8c\u786b\u952e\u76f8\u7b26\u7684\u8d28\u91cf\u672c\u8eab\u5e76\u4e0d\u80fd\u786e\u5b9a\u54ea\u4e9b\u534a\u80f1\u6c28\u9178\u76f8\u8fde\u3002Bachem \u7684 FAQ \u4e5f\u533a\u5206\u534a\u80f1\u6c28\u9178\u6b8b\u57fa\u4e0e\u7279\u522b\u8981\u6c42\u7684\u4e8c\u786b\u952e\u6392\u5217\u3002\u4e0b\u5355\u65f6\uff0c\u8bf7\u63d0\u4f9b\u9884\u671f\u7684\u534a\u80f1\u6c28\u9178\u914d\u5bf9\uff0c\u800c\u4e0d\u4ec5\u4ec5\u662f\u5e8f\u5217\u4e2d\u7684\u5b57\u6bcdC\u3002\u8be2\u95ee\u652f\u6301\u8be5\u6392\u5217\u7684\u8bc1\u636e\uff0c\u4ee5\u53ca\u60a8\u7684\u7279\u5b9a\u7814\u7a76\u662f\u5426\u9700\u8981\u8fd8\u539f\u6001\u6bd4\u8f83\u3002<\/p>\n<h2>\u4e0e NHD \u7684\u4e0b\u4e00\u6b65<\/h2>\n<p>\u4ece <a href=\"https:\/\/nhdbio.com\/cn\/%e6%9c%8d%e5%8a%a1\/%e5%ae%9a%e5%88%b6%e8%82%bd%e5%90%88%e6%88%90\/\">\u4e8c\u786b\u952e\u80bd\u9879\u76ee<\/a>. \u5982\u9700\u62a5\u4ef7\uff0c <a href=\"https:\/\/nhdbio.com\/cn\/%e5%bc%95%e7%94%a8\/\">\u5c06\u60a8\u7684\u5e8f\u5217\u6216\u4ea7\u54c1\u540d\u79f0\u3001\u6570\u91cf\u53ca\u6240\u9700\u6587\u4ef6\u53d1\u9001\u81f3 NHD<\/a>\u3002\u4e0b\u5355\u524d\u8bf7\u786e\u8ba4\u5177\u4f53\u5f62\u5f0f\u3001\u53ef\u884c\u6027\u3001\u53ef\u63d0\u4f9b\u7684\u68c0\u6d4b\u53ca\u4ea4\u4ed8\u6761\u6b3e\u3002<\/p>\n<h2>\u6765\u6e90<\/h2>\n<ul>\n<li><a href=\"https:\/\/pml.nist.gov\/cgi-bin\/Compositions\/stand_alone.pl?ele=H\">NIST\uff1a\u6c22\u540c\u4f4d\u7d20\u8d28\u91cf<\/a><\/li>\n<li><a href=\"https:\/\/www.bachem.com\/knowledge-center\/faq-frequently-asked-questions\/\">Bachem\uff1a\u80bd\u89c4\u683c\u5e38\u89c1\u95ee\u9898<\/a><\/li>\n<\/ul>\n<p>\u516c\u5f00\u53c2\u8003\u6587\u732e\u89e3\u91ca\u4e86\u8be5\u4e3b\u9898\uff1b\u5b83\u4eec\u4e0d\u662f NHD \u6279\u6b21\u8bc1\u636e\u3002\u7814\u7a76\u6750\u6599\u4ec5\u4f9b\u5b9e\u9a8c\u5ba4\u7814\u7a76\u3001\u5f00\u53d1\u548c\u751f\u4ea7\u4f7f\u7528\uff0c\u4e0d\u5f97\u7528\u4e8e\u4eba\u7c7b\u6216\u517d\u533b\u7528\u9014\u3002<\/p>","protected":false},"excerpt":{"rendered":"<p>\u540c\u4e00\u80bd\u7684\u4e24\u4e2a\u7248\u672c\u53ef\u4ee5\u5177\u6709\u76f8\u540c\u7684\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u4f46\u7531\u4e8e\u5206\u5b50\u5185\u5f62\u6210\u4e86\u952e\u800c\u8d28\u91cf\u7565\u6709\u4e0d\u540c\u3002\u4e8c\u786b\u952e\u662f\u4e00\u4e2a\u4f8b\u5b50\uff1a\u5728\u7b80\u5355\u6c27\u5316\u6a21\u578b\u4e0b\uff0c\u5f62\u6210\u4e00\u4e2a\u952e\u4f1a\u79fb\u9664\u4e24\u4e2a\u6c22\u539f\u5b50\u3002<\/p>","protected":false},"author":1,"featured_media":3112,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"354","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and labeled calculations","pim_reviewer":"Codex AI editorial","pim_reviewed_date":"2026-10-04","pim_editorial_note":"Public data and labeled teaching calculations; not NHD batch results. Confirm project and document availability.","pim_review_status":"reviewed","pim_review_reason":"Source, arithmetic, identity and search-intent checks. No independent human technical review recorded.","pim_creation_method":"AI-assisted writing, source checks and illustrated cover.","pim_sources":"[{\"title\":\"NIST: hydrogen isotopic masses\",\"url\":\"https:\/\/pml.nist.gov\/cgi-bin\/Compositions\/stand_alone.pl?ele=H\",\"note\":\"Public reference, not NHD batch evidence.\"},{\"title\":\"Bachem: peptide specification FAQ\",\"url\":\"https:\/\/www.bachem.com\/knowledge-center\/faq-frequently-asked-questions\/\",\"note\":\"Public reference, not NHD batch evidence.\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3104","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>A disulfide bond changes peptide mass by about 2 Da. 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