{"id":5741,"date":"2026-11-10T07:55:49","date_gmt":"2026-11-10T07:55:49","guid":{"rendered":"https:\/\/nhdbio.com\/?p=5741"},"modified":"2026-11-10T08:17:07","modified_gmt":"2026-11-10T08:17:07","slug":"scratch-assay-cell-migration-proliferation-reading-guide","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/de\/scratch-assay-cell-migration-proliferation-reading-guide\/","title":{"rendered":"Einf\u00fchrung in das Lesen von Scratch-Assay-Bildern: Zellmigration und -proliferation unterscheiden"},"content":{"rendered":"<section class=\"nhd-cp-topic\" id=\"nhd-cp-topic-knowledge-20261110-14\" style=\"max-width:1200px;margin:32px auto;padding:0 20px\">\n<h2>Scratch Assays: Cell Migration, Proliferation and Closure<\/h2>\n<p class=\"nhd-cp-lead\">When the blank area in a culture dish narrows, it means cell coverage has changed. But the cells may have moved into the space, increased in number, or both. When reading peptide repair studies, this distinction matters.<\/p>\n<figure style=\"margin:28px 0\"><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-covers-cover-14.png\" alt=\"Scratch Assays: Cell Migration, Proliferation and Closure\" width=\"1536\" height=\"1024\" loading=\"lazy\" style=\"display:block;width:100%;height:auto;object-fit:contain;border-radius:14px\"><figcaption>Editorial illustration of the research topic, not an experimental photograph.<\/figcaption><\/figure>\n<h3>First, see how the assay creates a blank area<\/h3>\n<p>In the classic scratch assay, a gap is created in a cultured cell layer, and then researchers observe how cells cover that area over a set period. The methods paper by Liang et al. describes this approach to studying migration. The blank area is not a human wound, and closure in a culture dish does not mean tissue repair has been completed. Cell type, culture conditions, initial density, and imaging time can all affect what is observed. <a href=\"#knowledge-20261110-14-source-1\">[1]<\/a><\/p>\n<h3>Calculate the closure percentage from area<\/h3>\n<p>A teaching example: the starting blank area is 1.00 mm\u00b2, and at a given time point 0.60 mm\u00b2 remains, so the area closure percentage = (1.00\u22120.60) \u00f7 1.00 \u00d7 100% = 40%. If another group starts with an area of 0.80 mm\u00b2, you cannot directly compare the final remaining areas of the two groups. The starting state and normalization rules need to be consistent. The data in this example are entirely hypothetical and do not represent migration speed, cell number, or any real effect of any peptide.<\/p>\n<figure style=\"margin:28px 0\"><img decoding=\"async\" class=\"nhd-localized-figure\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-en_US.png\" data-nhd-img-zh=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-zh_CN.png\" data-nhd-img-en=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-en_US.png\" data-nhd-img-de=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-de_DE.png\" data-nhd-img-es=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-es_ES.png\" data-nhd-img-fr=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-fr_FR.png\" data-nhd-img-pt=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-pt_BR.png\" data-nhd-img-ja=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-ja.png\" data-nhd-img-ar=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-14-ar.png\" alt=\"Teaching example: 40% area closure\" loading=\"lazy\" width=\"1600\" height=\"1500\" style=\"width:100%;height:auto;object-fit:contain;display:block\"><figcaption>Completely hypothetical area calculation; it cannot distinguish migration from proliferation on its own, and it does not represent a human repair effect.<\/figcaption><\/figure>\n<details>\n<summary>Teaching example: 40% area closure<\/summary>\n<ul>\n<li><strong>Starting blank area A\u2080<\/strong>: 1.00 mm\u00b2<\/li>\n<li><strong>Blank area at observation time A\u209c<\/strong>: 0.60 mm\u00b2<\/li>\n<li><strong>Area closure percentage<\/strong>: (A\u2080 \u2212 A\u209c) \u00f7 A\u2080 \u00d7 100% = 40%<\/li>\n<\/ul>\n<\/details>\n<h3>Both movement and proliferation make the blank smaller<\/h3>\n<p>Cells moving along the surface can cover the gap; cell division increasing numbers can also change coverage. To discuss the contribution of migration, you need to combine an appropriate observation time, an assessment of proliferation, and necessary controls. If an anti-proliferative treatment is used, you also need to assess whether it affects cell viability and migration itself. You cannot assume that other effects have completely disappeared just because some treatment was applied.<\/p>\n<h3>Before comparing photos, check comparability<\/h3>\n<p>Are the initial gaps similar? Were the same cells, culture medium, solvent, and observation intervals used? Were images taken from pre-specified positions? Are the area segmentation and boundary rules consistent? If you pick only the fields with the best closure, the results may deviate from the performance of the whole group. Keeping the original images, field numbers, and analysis rules helps others verify the work, rather than leaving only the final composite.<\/p>\n<h3>Multiple photos do not necessarily equal multiple independent samples<\/h3>\n<p>Taking ten photos of the same culture well mainly adds observational information about that well; it does not equal ten independent experiments. Study design needs to distinguish fields, culture wells, independent preparations, or cell sources, and statistical analysis should match the hierarchy. When describing results, state clearly what was measured and what the unit of comparison was; &quot;more closure&quot; cannot be expanded on its own into &quot;promotes human wound healing.&quot;<\/p>\n<h3>How to relate GHK-Cu and BPC-157 materials<\/h3>\n<p>These materials may appear in various repair-related studies, but the cells, treatments, and results in each paper need to be checked individually. When purchasing, first confirm the actual molecular form and batch documentation; when running experiments, keep records of preparation, controls, and observations. Product cards serve as an entry point for further verification; do not turn a result from cultured cells into a human efficacy claim for that product.<\/p>\n<h3>Original sources<\/h3>\n<ol class=\"nhd-cp-source\">\n<li id=\"knowledge-20261110-14-source-1\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/17406593\/\" target=\"_blank\" rel=\"noopener noreferrer\">Scratch assay original protocol \u00b7 PMID 17406593<\/a><\/li>\n<\/ol>\n<p class=\"nhd-cp-source\">Check the study conditions and material records separately. Calculations labeled as examples are not batch test results.<\/p>\n<h3>Related research materials<\/h3>\n<div class=\"nhd-cp-products\" style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(min(100%,240px),1fr));gap:20px\"><a class=\"nhd-cp-product\" href=\"https:\/\/nhdbio.com\/products\/bpc-157\/\" style=\"display:block;border:1px solid #dce4ed;border-radius:14px;padding:24px;background:#f7f9fb;color:#102b49\"><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/08\/product-family-mockups\/bpc-157-product-family-mockup-20260828.webp\" alt=\"BPC-157\" width=\"1536\" height=\"1024\" loading=\"lazy\" style=\"width:100%;height:auto;max-height:200px;object-fit:contain;display:block;margin:0 auto 16px\"><strong style=\"display:block;font-size:22px;margin-bottom:10px\">BPC-157<\/strong><span>View product information and specifications \u2192<\/span><\/a><a class=\"nhd-cp-product\" href=\"https:\/\/nhdbio.com\/products\/ghk-cu\/\" style=\"display:block;border:1px solid #dce4ed;border-radius:14px;padding:24px;background:#f7f9fb;color:#102b49\"><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/08\/product-family-mockups\/ghk-cu-product-family-mockup-20260828.webp\" alt=\"GHK-Cu\" width=\"1536\" height=\"1024\" loading=\"lazy\" style=\"width:100%;height:auto;max-height:200px;object-fit:contain;display:block;margin:0 auto 16px\"><strong style=\"display:block;font-size:22px;margin-bottom:10px\">GHK-Cu<\/strong><span>View product information and specifications \u2192<\/span><\/a><\/div>\n<p><a class=\"nhd-cp-download\" href=\"https:\/\/nhdbio.com\/quote\/\">Enquire about products and information \u2192<\/a><\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Wenn die freie Fl\u00e4che in der Kulturschale schmaler wird, bedeutet das, dass sich die Zellbedeckung ver\u00e4ndert hat. Die Zellen k\u00f6nnten dorthin gewandert sein, sich vermehrt haben oder beides. Beim Lesen von Peptid-Reparaturstudien ist dieser Unterschied wichtig.<\/p>","protected":false},"author":1,"featured_media":5460,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"318,329","pim_article_type":"Practical research guide","pim_evidence_level":"Preclinical research","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-11-10","pim_editorial_note":"Editorial illustrations are not experimental photographs. Teaching values are labeled.","pim_review_status":"reviewed","pim_review_reason":"Reviewed source scope, numerical examples, natural Chinese, product links and content layout.","pim_creation_method":"AI-assisted writing","pim_sources":"[{\"title\":\"Scratch assay original protocol \u00b7 PMID 17406593\",\"note\":\"Scratch assay original protocol \u00b7 PMID 17406593\",\"url\":\"https:\/\/pubmed.ncbi.nlm.nih.gov\/17406593\/\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-5741","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Scratch Assays: Cell Migration, Proliferation and Closure<\/title>\n<meta name=\"description\" content=\"Calculate scratch closure from area measurements. 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