{"id":2620,"date":"2026-09-23T12:07:01","date_gmt":"2026-09-23T12:07:01","guid":{"rendered":"https:\/\/nhdbio.com\/?p=2620"},"modified":"2026-10-11T07:37:52","modified_gmt":"2026-10-11T07:37:52","slug":"courbes-dun-test-dadsorption-par-dilution-en-serie-de-peptides","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/fr\/peptide-serial-dilution-adsorption-assay-curves\/","title":{"rendered":"Dilution S\u00e9rique du Peptide : Suivez Chaque Tube et Position de Plaque"},"content":{"rendered":"

A row of tubes labeled 100, 50 and 25 \u00b5M looks straightforward. The difficulty is remembering which solution moves where, and how much remains. We will follow one twofold dilution series from the first tube to the plate.<\/p>\n

Pourquoi des volumes \u00e9gaux divisent la concentration par deux<\/h2>\n

Start with 100 \u00b5L at 100 \u00b5M and add 100 \u00b5L of suitable diluent. The final volume is 200 \u00b5L. If no material is lost, the amount of peptide stays the same while the volume doubles, so the concentration becomes 50 \u00b5M.<\/p>\n

100 \u00b5M \u00d7 100 \u00b5L \u00f7 200 \u00b5L = 50 \u00b5M<\/p>\n

\"Figure
Figure 1. Des volumes \u00e9gaux de solution et de diluant donnent une dilution au demi.<\/figcaption><\/figure>\n

Suivez les quatre tubes suivants<\/h2>\n

Keep enough 100 \u00b5M solution in tube 1. Put 100 \u00b5L of diluent in each of tubes 2\u20135. Transfer 100 \u00b5L from tube 1 into tube 2 and mix; then transfer 100 \u00b5L from tube 2 into tube 3 and mix. Continue in that order.<\/p>\n

The concentrations are 100, 50, 25, 12.5 and 6.25 \u00b5M. Mix before taking the aliquot for the next tube, so the transferred liquid represents the newly diluted solution.<\/p>\n

\"Figure
Figure 2. Chaque transfert rend la solution suivante deux fois moins concentr\u00e9e.<\/figcaption><\/figure>\n

Comptez le liquide restant dans chaque tube<\/h2>\n

Tube 2 contains 200 \u00b5L after mixing, but only 100 \u00b5L after you transfer half to tube 3. The same applies to the later transfer tubes. The final tube remains at 200 \u00b5L if you do not remove an aliquot.<\/p>\n

That difference matters when you plan how many wells each tube can fill. The first tube\u2019s remaining volume also depends on how much starting stock you prepared.<\/p>\n

Une solution de travail peut \u00eatre dilu\u00e9e \u00e0 nouveau dans le puits<\/h2>\n

If a 50 \u00b5M working solution is mixed with an equal volume of another solution containing no peptide, the final concentration is 25 \u00b5M. The concentration on the tube is not automatically the concentration in the well.<\/p>\n

Dessinez un plan de plaque indiquant la concentration, les r\u00e9plicats et les puits t\u00e9moins. Cela permet de relier le calcul de dilution \u00e0 la disposition r\u00e9elle.<\/p>\n

\"Figure
Figure 3. Un plan de plaque aide \u00e0 garder ensemble les \u00e9tapes de dilution, les r\u00e9plicats et les t\u00e9moins.<\/figcaption><\/figure>\n

V\u00e9rifiez une \u00e9tape suppl\u00e9mentaire<\/h2>\n
Mix 80 \u00b5L of a 25 \u00b5M solution with 80 \u00b5L of diluent. What concentration results?<\/strong><\/p>\n
\nVoir la r\u00e9ponse<\/summary>\n

12.5 \u00b5M. The final volume is 160 \u00b5L. If you then remove 80 \u00b5L, the remaining solution is still 12.5 \u00b5M, but only 80 \u00b5L remains.<\/p>\n<\/details>\n<\/div>\n

Le calcul suppose que le peptide reste dissous et n'est pas perdu dans le r\u00e9cipient. Si la r\u00e9cup\u00e9ration est importante \u00e0 faibles concentrations, \u00e9tudiez ce point s\u00e9par\u00e9ment.<\/p>\n

Utilisation d'un plan de dilution avec KPV ou LL-37<\/h2>\n

Lorsque vous planifiez des dilutions de KPV ou LL-37, v\u00e9rifiez la concentration de la solution m\u00e8re et le solvant avant de commencer. Conservez le tableau de dilution avec la forme du produit et les d\u00e9tails du lot afin qu'un lot ult\u00e9rieur puisse \u00eatre compar\u00e9 dans les m\u00eames conditions pr\u00e9vues.<\/p>\n

LL-37<\/a> \/ KPV<\/a><\/p>\n

Discutez des exigences relatives au mat\u00e9riel et aux documents avec NHD<\/a> \u00b7 Poursuivre avec le guide analytique<\/a><\/p>\n