{"id":5708,"date":"2026-11-10T07:55:26","date_gmt":"2026-11-10T07:55:26","guid":{"rendered":"https:\/\/nhdbio.com\/?p=5708"},"modified":"2026-11-10T07:59:56","modified_gmt":"2026-11-10T07:59:56","slug":"peptide-spike-recovery-calculation-worked-example","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/fr\/peptide-spike-recovery-calculation-worked-example\/","title":{"rendered":"Comment calculer le taux de r\u00e9cup\u00e9ration par ajout dos\u00e9 : suivons un jeu de donn\u00e9es"},"content":{"rendered":"<section class=\"nhd-cp-topic\" id=\"nhd-cp-topic-knowledge-20261110-10\" style=\"max-width:1200px;margin:32px auto;padding:0 20px\">\n<h2>Spike Recovery: A Worked Peptide Testing Example<\/h2>\n<p class=\"nhd-cp-lead\">You add a known amount of the target substance to a sample\u2014does the extra amount you measure match what you added? That is the question a spike recovery experiment usually asks. It can help evaluate a method, but it is not a button that automatically corrects every sample.<\/p>\n<figure style=\"margin:28px 0\"><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-covers-cover-10.png\" alt=\"Spike Recovery: A Worked Peptide Testing Example\" width=\"1536\" height=\"1024\" loading=\"lazy\" style=\"display:block;width:100%;height:auto;object-fit:contain;border-radius:14px\"><figcaption>Editorial illustration of the research topic, not an experimental photograph.<\/figcaption><\/figure>\n<h3>First understand what &#039;spiking&#039; adds<\/h3>\n<p>Spiking usually means adding a reference material with a clearly defined identity and quantity to a sample, then putting it through the specified processing and testing. What is added is a known target substance, not just any bottle of material with the same name. The experiment needs to know the reference material&#039;s content basis, the amount added, and the time point of spiking. Different matrices and concentration levels may behave differently, and results under one condition cannot summarize all samples.<\/p>\n<h3>The easiest thing to miss is the amount already in the original sample<\/h3>\n<p>Suppose the unspiked sample measures 10.0 \u03bcg, the spiked sample measures 14.5 \u03bcg, the reference amount added to the corresponding sample is 5.0 \u03bcg, and all results have been converted to the same comparable basis. Recovery = (14.5 \u2212 10.0) \u00f7 5.0 \u00d7 100% = 90%. If you divide 14.5 directly by 5.0, you count the target substance already present in the original sample as part of the &#039;recovery.&#039; These three numbers are a teaching hypothesis, not actual batch data.<\/p>\n<figure style=\"margin:28px 0\"><img decoding=\"async\" class=\"nhd-localized-figure\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-en_US.png\" data-nhd-img-zh=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-zh_CN.png\" data-nhd-img-en=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-en_US.png\" data-nhd-img-de=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-de_DE.png\" data-nhd-img-es=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-es_ES.png\" data-nhd-img-fr=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-fr_FR.png\" data-nhd-img-pt=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-pt_BR.png\" data-nhd-img-ja=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-ja.png\" data-nhd-img-ar=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/11\/nhd-20261110-figures-10-ar.png\" alt=\"Teaching example: subtract the original sample first, then divide by the amount added\" loading=\"lazy\" width=\"1600\" height=\"1500\" style=\"width:100%;height:auto;object-fit:contain;display:block\"><figcaption>Completely hypothetical data; 90% is not a pass limit set on this page and does not represent actual NHD results.<\/figcaption><\/figure>\n<details>\n<summary>Teaching example: subtract the original sample first, then divide by the amount added<\/summary>\n<ul>\n<li><strong>Unspiked \/ after spiking<\/strong>: 10.0 \u03bcg \/ 14.5 \u03bcg (converted to the same basis)<\/li>\n<li><strong>Additional amount added<\/strong>: 5.0 \u03bcg<\/li>\n<li><strong>Spike recovery<\/strong>: (14.5 \u2212 10.0) \u00f7 5.0 \u00d7 100% = 90%<\/li>\n<\/ul>\n<\/details>\n<h3>Adding a solution can also change the denominator and the volume<\/h3>\n<p>If results are reported as concentration, adding a standard solution changes the volume, so the dilution relationship between the original and spiked samples must be handled together. You can first convert everything to the mass of the corresponding sample and then make the comparison above; or you can use a confirmed concentration-and-volume correction approach. Without a record of the final volume, a set of concentration numbers alone may not be enough to calculate it. Whether the control sample needs the same amount of blank solvent added should also be confirmed at the design stage.<\/p>\n<h3>The spiking time point determines which steps it covers<\/h3>\n<p>If spiking is done before extraction, the result may include the influence of more sample-preparation steps; if it is added only after extraction, it cannot be used to show that the earlier extraction had no loss. For materials such as GHK-Cu that involve chemical state, you also need to confirm whether the added reference material matches the object to be measured and whether processing changed the measured state. A single recovery number does not automatically answer these identity questions.<\/p>\n<h3>Whether 90% passes depends first on the purpose<\/h3>\n<p>This article does not set a unified acceptance range. Evaluation should combine the method&#039;s purpose, concentration level, repeatability, and pre-specified criteria. If recovery is abnormal, first check the standard material, volume, matrix interference, sample-preparation loss, and instrument response; do not directly divide all results by 0.90 and then claim the true content has been obtained. Whether correction is allowed and how to correct require the corresponding method basis. <a href=\"#knowledge-20261110-10-source-1\">[1]<\/a><\/p>\n<h3>Write the results as a record others can recalculate<\/h3>\n<p>Record the unspiked result, the spiked result, the known amount added, volume handling, spiking time point, and calculation formula. When submitting materials such as glutathione or GHK-Cu for testing, you can state whether you care most about quantitative bias or recovery at a certain step, and have the laboratory confirm the design. Catalog materials are not standard substances that naturally have value-assignment qualification; the uses of reference materials and ordinary research materials should be confirmed separately.<\/p>\n<h3>Original sources<\/h3>\n<ol class=\"nhd-cp-source\">\n<li id=\"knowledge-20261110-10-source-1\"><a href=\"https:\/\/www.fda.gov\/regulatory-information\/search-fda-guidance-documents\/q2r2-validation-analytical-procedures\" target=\"_blank\" rel=\"noopener noreferrer\">FDA \u00b7 ICH Q2(R2) validation of analytical procedures<\/a><\/li>\n<\/ol>\n<p class=\"nhd-cp-source\">Check the study conditions and material records separately. Calculations labeled as examples are not batch test results.<\/p>\n<h3>Related research materials<\/h3>\n<div class=\"nhd-cp-products\" style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(min(100%,240px),1fr));gap:20px\"><a class=\"nhd-cp-product\" href=\"https:\/\/nhdbio.com\/products\/glutathione\/\" style=\"display:block;border:1px solid #dce4ed;border-radius:14px;padding:24px;background:#f7f9fb;color:#102b49\"><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/08\/product-family-mockups\/glutathione-product-family-mockup-20260828.webp\" alt=\"Glutathione\" width=\"1536\" height=\"1024\" loading=\"lazy\" style=\"width:100%;height:auto;max-height:200px;object-fit:contain;display:block;margin:0 auto 16px\"><strong style=\"display:block;font-size:22px;margin-bottom:10px\">Glutathione<\/strong><span>View product information and specifications \u2192<\/span><\/a><a class=\"nhd-cp-product\" href=\"https:\/\/nhdbio.com\/products\/ghk-cu\/\" style=\"display:block;border:1px solid #dce4ed;border-radius:14px;padding:24px;background:#f7f9fb;color:#102b49\"><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/08\/product-family-mockups\/ghk-cu-product-family-mockup-20260828.webp\" alt=\"GHK-Cu\" width=\"1536\" height=\"1024\" loading=\"lazy\" style=\"width:100%;height:auto;max-height:200px;object-fit:contain;display:block;margin:0 auto 16px\"><strong style=\"display:block;font-size:22px;margin-bottom:10px\">GHK-Cu<\/strong><span>View product information and specifications \u2192<\/span><\/a><\/div>\n<p><a class=\"nhd-cp-download\" href=\"https:\/\/nhdbio.com\/quote\/\">Enquire about products and information \u2192<\/a><\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Vous ajoutez une quantit\u00e9 connue de la substance cible dans un \u00e9chantillon, et la quantit\u00e9 suppl\u00e9mentaire mesur\u00e9e correspond-elle \u00e0 la quantit\u00e9 ajout\u00e9e ? C&#8217;est la question que pose souvent l&#8217;essai de r\u00e9cup\u00e9ration par ajout dos\u00e9. Il aide \u00e0 \u00e9valuer une m\u00e9thode, mais ce n&#8217;est pas un bouton qui \u00ab corrige automatiquement \u00bb chaque \u00e9chantillon.<\/p>","protected":false},"author":1,"featured_media":5459,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"333,329","pim_article_type":"Practical research guide","pim_evidence_level":"Technical guidance with labeled teaching examples","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-11-10","pim_editorial_note":"Editorial illustrations are not experimental photographs. Teaching values are labeled.","pim_review_status":"reviewed","pim_review_reason":"Reviewed source scope, numerical examples, natural Chinese, product links and content layout.","pim_creation_method":"AI-assisted writing","pim_sources":"[{\"title\":\"ICH Q2(R2): Validation of Analytical Procedures\",\"note\":\"Defines validation characteristics and distinguishes quantitative analytical purposes.\",\"url\":\"https:\/\/www.fda.gov\/regulatory-information\/search-fda-guidance-documents\/q2r2-validation-analytical-procedures\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-5708","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Spike Recovery: A Worked Peptide Testing Example<\/title>\n<meta name=\"description\" content=\"Calculate 90% spike recovery step by step. 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