{"id":4067,"date":"2026-10-11T06:33:31","date_gmt":"2026-10-11T06:33:31","guid":{"rendered":"https:\/\/nhdbio.com\/?page_id=4067"},"modified":"2026-10-15T03:21:40","modified_gmt":"2026-10-15T03:21:40","slug":"%e8%b3%aa%e9%87%8f%e6%bf%83%e5%ba%a6%e5%af%be%e3%83%a2%e3%83%ab%e6%bf%83%e5%ba%a6","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/ja\/analytical-methods\/mass-concentration-versus-molarity\/","title":{"rendered":"mg\/mL \u5bfe \u00b5M\uff1a\u540c\u3058\u30da\u30d7\u30c1\u30c9\u6eb6\u6db2\u3092\u8868\u30592\u3064\u306e\u65b9\u6cd5"},"content":{"rendered":"

Is 1 mg\/mL stronger than 500 \u00b5M? You cannot tell from those two numbers alone. They describe concentration in different ways. With the peptide\u2019s molecular weight, you can put both values into the same unit.<\/p>\n

mg\/mL\u306f\u91cd\u91cf\/\u4f53\u7a4d\u3068\u3057\u3066\u8aad\u307f\u307e\u3059<\/h2>\n

1 mg\/mL\u306e\u6fc3\u5ea6\u3068\u306f\u3001\u6eb6\u6db21\u30df\u30ea\u30ea\u30c3\u30c8\u30eb\u4e2d\u306b\u6570\u3048\u3066\u3044\u308b\u7269\u8cea\u304c1 mg\u542b\u307e\u308c\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3059\u3002\u305d\u306e\u6fc3\u5ea6\u3067\u306f\u30012 mL\u306b\u306f2 mg\u304c\u542b\u307e\u308c\u300110 mL\u306b\u306f10 mg\u304c\u542b\u307e\u308c\u307e\u3059\u3002<\/p>\n

\u7269\u8cea\u3092\u660e\u78ba\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u306f\u79e4\u91cf\u3057\u305f\u7c89\u672b\u3067\u3059\u304b\u3001\u305d\u308c\u3068\u3082\u76ee\u7684\u306e\u30da\u30d7\u30c1\u30c9\u306e\u6e2c\u5b9a\u91cf\u3067\u3059\u304b\uff1f<\/p>\n

Read \u00b5M as molecular amount per volume<\/h2>\n

\u00b5M means micromolar: micromoles per litre. It is useful when comparing amounts of molecules. At the same mg\/mL, smaller molecules give more molecules per volume than larger ones.<\/p>\n

\u540c\u3058\u91cd\u3055\u306e\u30d3\u30fc\u30ba\u306e\u888b\u30922\u3064\u60f3\u50cf\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5c0f\u3055\u306a\u30d3\u30fc\u30ba\u306e\u888b\u306b\u306f\u3001\u3088\u308a\u591a\u304f\u306e\u500b\u3005\u306e\u30d3\u30fc\u30ba\u304c\u5165\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\"\u56f31\u3002\u540c\u3058\u7c89\u672b\u8cea\u91cf\u3067\u3082\u7570\u306a\u308b\u5206\u5b50\u6570\u3092\u542b\u3080\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\"
\u56f31\u3002\u540c\u3058\u91cd\u3055\u304c\u5e38\u306b\u540c\u3058\u5206\u5b50\u6570\u3092\u610f\u5473\u3059\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n

Convert 1 mg\/mL into \u00b5M<\/h2>\n

Take an example peptide with a molecular weight of 2,000 g\/mol. First, 1 mg\/mL is the same as 1 g\/L. Divide by 2,000 to get 0.0005 mol\/L, then convert to micromolar: 500 \u00b5M.<\/p>\n

\u00b5M = mg\/mL \u00d7 1,000,000 \u00f7 molecular weight (g\/mol)<\/p>\n

\u74f6\u306e\u4e2d\u8eab\u306f\u4f55\u3082\u5909\u308f\u3063\u3066\u3044\u307e\u305b\u3093\u3002\u540c\u3058\u6fc3\u5ea6\u3092\u5225\u306e\u5358\u4f4d\u3067\u8868\u3057\u305f\u3060\u3051\u3067\u3059\u3002<\/p>\n

\u5225\u306e\u5206\u5b50\u91cf\u3092\u8a66\u3059<\/h2>\n

At 1,000 g\/mol, the same 1 mg\/mL becomes 1,000 \u00b5M. At 2,000 g\/mol, it was 500 \u00b5M. The first solution has twice as many molecules per volume, even though the weight concentrations match.<\/p>\n

\"Figure
Figure 2. At 1 mg\/mL, the example molecular weights give 1,000 \u00b5M and 500 \u00b5M.<\/figcaption><\/figure>\n
\u5206\u5b50\u91cf 4,000 g\/mol \u306e\u3068\u304d\u30010.2 mg\/mL \u306f\u3044\u304f\u3089\u3067\u3059\u304b\uff1f<\/strong><\/p>\n
\n\u7b54\u3048\u3092\u898b\u308b<\/summary>\n

0.2 \u00d7 1,000,000 \u00f7 4,000 = 50 \u00b5M.<\/p>\n<\/details>\n<\/div>\n

\u6fc3\u5ea6\u306f\u7dcf\u91cf\u3068\u306f\u5225\u306b\u6271\u3046<\/h2>\n

Two tubes at 250 \u00b5M can have different total amounts if one holds 1 mL and the other holds 10 mL. Concentration says how much is present per volume; total amount also needs the volume.<\/p>\n

\u3053\u306e\u63db\u7b97\u3067\u306f\u3001\u30da\u30d7\u30c1\u30c9\u304c\u5b8c\u5168\u306b\u6eb6\u89e3\u3057\u305f\u304b\u3001\u3042\u308b\u3044\u306f\u5206\u89e3\u305b\u305a\u306b\u7dad\u6301\u3055\u308c\u305f\u304b\u3092\u77e5\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u305b\u3093\u3002\u305d\u308c\u3089\u306f\u5225\u306e\u78ba\u8a8d\u4e8b\u9805\u3067\u3059\u3002<\/p>\n

\"\u56f33\u3002\u6fc3\u5ea6\u306e\u63db\u7b97\u306f\u6eb6\u89e3\u3057\u305f\u6a19\u7684\u542b\u91cf\u3092\u8a3c\u660e\u3057\u307e\u305b\u3093\u3002\"
\u56f33\u3002\u8a08\u7b97\u3055\u308c\u305f\u6fc3\u5ea6\u3068\u6e2c\u5b9a\u3055\u308c\u305f\u6eb6\u6db2\u6fc3\u5ea6\u306f\u7570\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/figcaption><\/figure>\n

KPV\u307e\u305f\u306fLL-37\u6fc3\u5ea6\u306e\u6bd4\u8f03<\/h2>\n

KPV\u307e\u305f\u306fLL-37\u306b\u3064\u3044\u3066\u306f\u3001\u6fc3\u5ea6\u5358\u4f4d\u3068\u6b63\u78ba\u306a\u88fd\u54c1\u5f62\u614b\u3092\u4e00\u7dd2\u306b\u8a18\u9332\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u7814\u7a76\u8ad6\u6587\u3068\u88fd\u54c1\u8a18\u9332\u3092\u6bd4\u8f03\u3059\u308b\u5834\u5408\u306f\u3001\u307e\u305a\u6b63\u3057\u3044\u5206\u5b50\u91cf\u3092\u7528\u3044\u3066\u4e21\u65b9\u3092\u540c\u3058\u5358\u4f4d\u306b\u63db\u7b97\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

LL-37<\/a> \/ KPV<\/a><\/p>\n

\u8cc7\u6750\u304a\u3088\u3073\u6587\u66f8\u306e\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u3068\u76f8\u8ac7\u3059\u308b<\/a> \u00b7 \u5206\u6790\u30ac\u30a4\u30c9\u3092\u7d9a\u3051\u308b<\/a><\/p>\n