{"id":4121,"date":"2026-10-11T06:37:06","date_gmt":"2026-10-11T06:37:06","guid":{"rendered":"https:\/\/nhdbio.com\/?page_id=4121"},"modified":"2026-10-11T07:41:09","modified_gmt":"2026-10-11T07:41:09","slug":"%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e6%a4%9c%e9%87%8f%e7%b7%9a%e3%81%ae%e8%a8%88%e7%ae%97%e4%be%8b","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/ja\/analytical-methods\/peptide-calibration-curve-worked-example\/","title":{"rendered":"\u30da\u30d7\u30c1\u30c9\u691c\u91cf\u7dda\uff1a\u672a\u77e5\u6fc3\u5ea6\u306e\u8a08\u7b97"},"content":{"rendered":"

\u6a5f\u5668\u306f6.2\u306e\u30b7\u30b0\u30ca\u30eb\u3092\u51fa\u3057\u307e\u3059\u304c\u3001\u5fc5\u8981\u306a\u306e\u306f\u6fc3\u5ea6\u3067\u3059\u3002\u3069\u3046\u3059\u308c\u3070\u4e00\u65b9\u304b\u3089\u4ed6\u65b9\u3078\u5909\u63db\u3067\u304d\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u307e\u305a\u65e2\u77e5\u306e\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u305d\u308c\u3089\u306e\u30b7\u30b0\u30ca\u30eb\u304b\u3089\u691c\u91cf\u7dda\u304c\u5f97\u3089\u308c\u3001\u9069\u5207\u306a\u6761\u4ef6\u4e0b\u3067\u672a\u77e5\u8a66\u6599\u306b\u4f7f\u3048\u307e\u3059\u3002<\/p>\n

\u6fc3\u5ea6\u304c\u308f\u304b\u3063\u3066\u3044\u308b\u8a66\u6599\u304b\u3089\u59cb\u3081\u307e\u3059<\/h2>\n

Our teaching standards are 0, 1, 2, 4 and 8 \u00b5M. Measure them using the chosen method, then plot concentration on the horizontal axis and signal on the vertical axis.<\/p>\n

\u6fc3\u5ea6\u306f\u4fe1\u983c\u3067\u304d\u308b\u3082\u306e\u3067\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002\u8aa4\u3063\u3066\u8abf\u88fd\u3057\u305f\u6a19\u6e96\u54c1\u304b\u3089\u4f5c\u3063\u305f\u691c\u91cf\u7dda\u306f\u3001\u304d\u308c\u3044\u306b\u898b\u3048\u3066\u3082\u8aa4\u3063\u305f\u7d50\u679c\u3092\u4e0e\u3048\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u6a19\u6e96\u54c1\u3068\u672a\u77e5\u8a66\u6599\u306b\u306f\u3001\u9069\u5207\u306b\u6bd4\u8f03\u3067\u304d\u308b\u8abf\u88fd\u6761\u4ef6\u3068\u6e2c\u5b9a\u6761\u4ef6\u3082\u5fc5\u8981\u3067\u3059\u3002<\/p>\n

\"Figure
\u56f31\u3002\u65e2\u77e5\u306e\u6fc3\u5ea6\u304c\u6e2c\u5b9a\u30b7\u30b0\u30ca\u30eb\u3068\u306e\u95a2\u4fc2\u3092\u78ba\u7acb\u3057\u307e\u3059\u3002<\/figcaption><\/figure>\n

y = 2x + 0.2 \u306f\u4f55\u3092\u610f\u5473\u3059\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002<\/h2>\n

Suppose the fitted relationship is y = 2x + 0.2. Here x is concentration in \u00b5M, and y is the instrument response in example units. Each extra \u00b5M adds two response units; the intercept is 0.2.<\/p>\n

To find x from a signal, reverse the calculation: subtract 0.2, then divide by two. Follow the method\u2019s blank correction; do not subtract the same background twice.<\/p>\n

\u672a\u77e5\u306e\u30b7\u30b0\u30ca\u30eb\u3092\u4f7f\u7528\u3059\u308b<\/h2>\n

For y = 6.2, x = (6.2 \u2212 0.2) \u00f7 2 = 3 \u00b5M. This lies within the illustrated 0\u20138 \u00b5M span.<\/p>\n

If the unknown is outside the method\u2019s established working range, do not assume the line remains valid indefinitely. The laboratory may need a suitable dilution or another validated measurement arrangement.<\/p>\n

\"Figure
Figure 2. In this teaching model, a response of 6.2 corresponds to 3 \u00b5M.<\/figcaption><\/figure>\n

\u5143\u306e\u8a66\u6599\u306f\u5e0c\u91c8\u3055\u308c\u307e\u3057\u305f\u304b\uff1f<\/h2>\n

If the sample was diluted fivefold before measurement, the measured solution is 3 \u00b5M, while the original is 3 \u00d7 5 = 15 \u00b5M, assuming no loss.<\/p>\n

\u8a66\u6599 1 \u90e8\u306b\u5e0c\u91c8\u6db2 4 \u90e8\u3092\u52a0\u3048\u308b\u3068\u5168\u4f53\u3067 5 \u90e8\u306b\u306a\u308a\u307e\u3059\u3002\u3053\u308c\u306f 4 \u500d\u3067\u306f\u306a\u304f 5 \u500d\u5e0c\u91c8\u3067\u3059\u3002\u6700\u7d42\u4f53\u7a4d\u3068\u79fb\u3057\u305f\u8a66\u6599\u4f53\u7a4d\u3092\u8a18\u9332\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\"Figure
\u56f3 3\u3002\u5e0c\u91c8\u4fc2\u6570\u3092\u9069\u7528\u3057\u3066\u5143\u306e\u6fc3\u5ea6\u3092\u6c42\u3081\u307e\u3059\u3002<\/figcaption><\/figure>\n

\u307e\u3063\u3059\u3050\u306b\u898b\u3048\u308b\u7dda\u3067\u3082\u78ba\u8a8d\u304c\u5fc5\u8981\u3067\u3059<\/h2>\n

Not necessarily. A high R\u00b2 describes fit to the data; it does not alone prove accurate low-concentration results. Repeatability, background, recovery and interference also need appropriate checks.<\/p>\n

\u5fdc\u7b54\u306f 10.2 \u3067\u3001\u8a66\u6599\u306f 3 \u500d\u306b\u5e0c\u91c8\u3055\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u5143\u306e\u6fc3\u5ea6\u306f\u3044\u304f\u3089\u3067\u3057\u305f\u304b\uff1f<\/strong><\/p>\n
\n\u7b54\u3048\u3092\u898b\u308b<\/summary>\n

The measured solution is (10.2 \u2212 0.2) \u00f7 2 = 5 \u00b5M. The original is 5 \u00d7 3 = 15 \u00b5M.<\/p>\n<\/details>\n<\/div>\n

KPV\u307e\u305f\u306fLL-37\u6e2c\u5b9a\u306e\u8a08\u753b<\/h2>\n

KPV\u307e\u305f\u306fLL-37\u306e\u5b9a\u91cf\u3067\u306f\u3001\u76ee\u7684\u30da\u30d7\u30c1\u30c9\u306e\u91cf\u306e\u7d50\u679c\u304c\u5fc5\u8981\u306a\u306e\u304b\u3001\u30e1\u30bd\u30c3\u30c9\u7528\u306e\u9069\u5207\u306a\u6a19\u6e96\u7269\u8cea\u304c\u5fc5\u8981\u306a\u306e\u304b\u3092\u8aac\u660e\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30ab\u30bf\u30ed\u30b0\u88fd\u54c1\u306f\u81ea\u52d5\u7684\u306b\u8a8d\u8a3c\u6a19\u6e96\u7269\u8cea\u306b\u306a\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

KPV<\/a> \/ LL-37<\/a><\/p>\n

\u8cc7\u6750\u304a\u3088\u3073\u6587\u66f8\u306e\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u3068\u76f8\u8ac7\u3059\u308b<\/a> \u00b7 \u5206\u6790\u30ac\u30a4\u30c9\u3092\u7d9a\u3051\u308b<\/a><\/p>\n