{"id":4521,"date":"2026-10-13T16:13:50","date_gmt":"2026-10-13T16:13:50","guid":{"rendered":"https:\/\/nhdbio.com\/?page_id=4521"},"modified":"2026-10-13T16:13:50","modified_gmt":"2026-10-13T16:13:50","slug":"%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e3%83%95%e3%82%a3%e3%83%ab%e3%82%bf%e3%83%bc%e5%9b%9e%e5%8f%8e%e3%81%ae%e8%a8%88%e7%ae%97%e4%be%8b","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/ja\/analytical-methods\/peptide-filter-recovery-worked-example\/","title":{"rendered":"\u308d\u904e\u5f8c\u306b\u30da\u30d7\u30c1\u30c9\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u4f4e\u4e0b\u3059\u308b\uff1a\u30d5\u30a3\u30eb\u30bf\u30fc\u304c\u30da\u30d7\u30c1\u30c9\u3092\u4fdd\u6301\u3057\u3066\u3044\u308b\u306e\u304b\uff1f"},"content":{"rendered":"<section class=\"nhd-cp-topic\" id=\"nhd-cp-topic-knowledge-20261013-05\">\n<p class=\"nhd-cp-lead\">\u308d\u904e\u5f8c\u306e\u30b7\u30b0\u30ca\u30eb\u4f4e\u4e0b\u306f\u3001\u5fc5\u305a\u3057\u3082\u30da\u30d7\u30c1\u30c9\u304c\u3059\u3079\u3066\u819c\u306b\u5438\u7740\u3057\u305f\u3053\u3068\u3092\u610f\u5473\u3059\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u307e\u305a\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u3001\u5bb9\u91cf\u3001\u691c\u51fa\u6761\u4ef6\u3092\u63c3\u3048\u3066\u3001\u3069\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u30b5\u30f3\u30d7\u30eb\u304c\u5931\u308f\u308c\u305f\u306e\u304b\u3092\u628a\u63e1\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/nhd-knowledge-20261013-05.webp\" alt=\"\u3053\u306e\u30ac\u30a4\u30c9\u3067\u6271\u3046\u30c8\u30d4\u30c3\u30af\u306e\u30b3\u30df\u30c3\u30af\u30a4\u30e9\u30b9\u30c8\"><figcaption>\u7de8\u96c6\u7528\u30a4\u30e9\u30b9\u30c8\u3067\u3042\u308a\u3001\u5b9f\u9a13\u5199\u771f\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n<h3>\u307e\u305a\u30b7\u30b0\u30ca\u30eb\u3001\u6fc3\u5ea6\u3001\u56de\u53ce\u91cf\u3092\u533a\u5225\u3059\u308b<\/h3>\n<p>\u88c5\u7f6e\u306e\u30b7\u30b0\u30ca\u30eb\u306f\u6e2c\u5b9a\u5024\u3067\u3042\u308a\u3001\u6fc3\u5ea6\u306f\u5358\u4f4d\u4f53\u7a4d\u3042\u305f\u308a\u306e\u91cf\u3067\u3042\u308a\u3001\u56de\u53ce\u91cf\u306f\u3055\u3089\u306b\u5b9f\u969b\u306b\u56de\u53ce\u3057\u305f\u4f53\u7a4d\u3092\u639b\u3051\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002\u308d\u904e\u524d\u5f8c\u306e\u30d4\u30fc\u30af\u9ad8\u3055\u3060\u3051\u3092\u6bd4\u8f03\u3059\u308b\u3068\u3001\u691c\u51fa\u5fdc\u7b54\u3084\u4f53\u7a4d\u306e\u5909\u5316\u3092\u7269\u8cea\u306e\u640d\u5931\u3068\u8aa4\u8a8d\u3057\u3084\u3059\u3044\u3002<\/p>\n<p>\u8a66\u6599\u306f\u30e1\u30f3\u30d6\u30ec\u30f3\u3084\u30cf\u30a6\u30b8\u30f3\u30b0\u5185\u306b\u6b8b\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u3001\u8868\u9762\u306b\u5438\u7740\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u3001\u53d6\u308a\u6271\u3044\u4e2d\u306b\u6c88\u6bbf\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u3001\u691c\u51fa\u6761\u4ef6\u306b\u3088\u3063\u3066\u5fdc\u7b54\u304c\u5f31\u304f\u898b\u3048\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002\u30e1\u30f3\u30d6\u30ec\u30f3\u6750\u6599\u3001\u8a66\u6599\u3001\u6eb6\u5a92\u306e\u76f8\u4e92\u4f5c\u7528\u306f\u500b\u5225\u306b\u691c\u8a3c\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002<\/p>\n<h3>\u4eee\u60f3\u30c7\u30fc\u30bf\u306e\u4e00\u4f8b\uff1a\u6fc3\u5ea6\u56de\u53ce\u7387\u3068\u7dcf\u56de\u53ce\u7387\u306f\u540c\u3058\u3067\u306f\u306a\u3044<\/h3>\n<p>Before filtration: 1.00 mL, concentration 100 \u03bcg\/mL, total 100 \u03bcg. After filtration, actually collected 0.80 mL, concentration 80 \u03bcg\/mL, total 64 \u03bcg. Compared by concentration it is 80%, but compared by total it is 64%.<\/p>\n<p>This data only demonstrates how to calculate; it does not represent the true performance of any membrane. If concentration is converted from a detection method, the method&#039;s own calibration and applicability must also be reasonable.<\/p>\n<h3>\u6709\u7528\u306a\u308d\u904e\u524d\u5f8c\u306e\u6bd4\u8f03\u306f\u3069\u3046\u884c\u3046\u304b\uff1f<\/h3>\n<p>\u540c\u4e00\u306e\u5747\u8cea\u306a\u8a66\u6599\u304b\u3089\u5bfe\u7167\u90e8\u5206\u3068\u308d\u904e\u90e8\u5206\u3092\u5206\u53d6\u3059\u308b\u3002\u5bfe\u7167\u306f\u3001\u691c\u8a0e\u5bfe\u8c61\u306e\u308d\u904e\u5de5\u7a0b\u306e\u307f\u3092\u7701\u304d\u3001\u53ef\u80fd\u306a\u9650\u308a\u540c\u3058\u5f85\u6a5f\u6642\u9593\u3001\u5bb9\u5668\u79fb\u3057\u66ff\u3048\u3001\u5e0c\u91c8\u3092\u7d4c\u308b\u3079\u304d\u3067\u3042\u308b\u3002\u308d\u904e\u30c7\u30d0\u30a4\u30b9\u3001\u30ed\u30c3\u30c8\u3001\u51e6\u7406\u4f53\u7a4d\u3001\u5b9f\u969b\u306e\u56de\u53ce\u4f53\u7a4d\u3001\u6642\u9593\u3092\u8a18\u9332\u3059\u308b\u3002<\/p>\n<p>\u524d\u5f8c\u306e\u8a66\u6599\u3092\u6e2c\u5b9a\u3059\u308b\u969b\u306f\u3001\u65b9\u6cd5\u3068\u6ce8\u5165\u6761\u4ef6\u3092\u6bd4\u8f03\u53ef\u80fd\u306a\u72b6\u614b\u306b\u4fdd\u3064\u3053\u3068\u3002\u691c\u51fa\u30d6\u30e9\u30f3\u30af\u306f\u3001\u819c\u3084\u6eb6\u5a92\u304b\u3089\u306e\u5e72\u6e09\u3092\u9664\u5916\u3059\u308b\u306e\u306b\u5f79\u7acb\u3064\u3002\u6dfb\u52a0\u56de\u53ce\u3084\u305d\u306e\u4ed6\u306e\u5bfe\u7167\u304c\u5fc5\u8981\u304b\u3069\u3046\u304b\u306f\u3001\u65b9\u6cd5\u3068\u7814\u7a76\u76ee\u7684\u306b\u3088\u3063\u3066\u5224\u65ad\u3059\u3079\u304d\u3067\u3042\u308b\u3002<\/p>\n<h3>\u7d50\u679c\u304c\u51fa\u305f\u3089\u3001\u307e\u305a\u539f\u56e0\u3092\u7279\u5b9a\u3057\u3001\u305d\u306e\u5f8c\u3067\u6761\u4ef6\u3092\u5909\u66f4\u3059\u308b<\/h3>\n<p>\u56de\u53ce\u6db2\u91cf\u304c\u5c11\u306a\u3044\u5834\u5408\u306f\u3001\u307e\u305a\u88c5\u7f6e\u306e\u30db\u30fc\u30eb\u30c9\u30a2\u30c3\u30d7\u3068\u64cd\u4f5c\u8a18\u9332\u3092\u78ba\u8a8d\u3059\u308b\u3002\u6fc3\u5ea6\u304c\u4f4e\u4e0b\u3057\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u5438\u7740\u3001\u6c88\u6bbf\u3001\u307e\u305f\u306f\u691c\u51fa\u5fdc\u7b54\u306e\u5909\u5316\u3092\u8abf\u3079\u308b\u3002\u4e21\u65b9\u304c\u540c\u6642\u306b\u8d77\u3053\u308a\u3046\u308b\u305f\u3081\u3001\u5358\u4e00\u306e\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3067\u306f\u539f\u56e0\u3092\u7279\u5b9a\u3067\u304d\u306a\u3044\u3002<\/p>\n<p>\u819c\u7d20\u6750\u3001\u51e6\u7406\u91cf\u3001\u305d\u306e\u4ed6\u306e\u5de5\u7a0b\u3092\u5909\u66f4\u3057\u305f\u5f8c\u306f\u3001\u56de\u53ce\u7387\u3068\u5206\u6790\u6027\u80fd\u3092\u518d\u691c\u8a3c\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002\u4e00\u3064\u306e\u6539\u5584\u3057\u305f\u30b7\u30b0\u30ca\u30eb\u304b\u3089\u3001\u305d\u306e\u819c\u304c\u3059\u3079\u3066\u306e\u30da\u30d7\u30c1\u30c9\u3068\u3059\u3079\u3066\u306e\u6fc3\u5ea6\u306b\u9069\u3057\u3066\u3044\u308b\u3068\u63a8\u8ad6\u3057\u3066\u306f\u306a\u3089\u306a\u3044\u3002<\/p>\n<h3>Write \u201ceffective recovery\u201d as a record that can be rechecked<\/h3>\n<p>\u5831\u544a\u306b\u306f\u3001\u56de\u53ce\u7387\u304c\u6fc3\u5ea6\u3001\u30d4\u30fc\u30af\u9762\u7a4d\u3001\u307e\u305f\u306f\u7dcf\u91cf\u306e\u3069\u308c\u3067\u8a08\u7b97\u3055\u308c\u305f\u304b\u3001\u307e\u305f\u5bb9\u91cf\u3068\u5e0c\u91c8\u304c\u88dc\u6b63\u3055\u308c\u305f\u304b\u3069\u3046\u304b\u3092\u660e\u78ba\u306b\u8a18\u8f09\u3059\u3079\u304d\u3067\u3042\u308b\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u4ed6\u8005\u304c\u540c\u3058\u7d50\u679c\u3092\u518d\u8a08\u7b97\u3067\u304d\u3001\u640d\u5931\u306e\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u3068\u5f8c\u7d9a\u306e\u5b9f\u9a13\u304c\u3064\u306a\u304c\u308b\u3002<\/p>\n<figure class=\"nhd-cp-diagram\"><figcaption class=\"nhd-cp-flow-title\">\u4eee\u60f3\u7684\u306a\u308d\u904e\u524d\u5f8c\u306e\u30c7\u30fc\u30bf<\/figcaption><div class=\"nhd-cp-steps\">\n<div class=\"nhd-cp-step\"><b>\u308d\u904e\u524d<\/b><span>Concentration: 100 \u03bcg\/mL<\/span><span>\u5bb9\u91cf: 1.00 mL<\/span><span>Total: 100 \u03bcg<\/span><\/div>\n<div class=\"nhd-cp-step\"><b>\u308d\u904e\u5f8c<\/b><span>Concentration: 80 \u03bcg\/mL<\/span><span>\u5bb9\u91cf: 0.80 mL<\/span><span>Total: 64 \u03bcg<\/span><\/div>\n<\/div><figcaption>\u6559\u80b2\u7528\u307e\u3068\u3081\u3002\u8aac\u660e\u3068\u539f\u5178\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u306f\u7e2e\u5c3a\u901a\u308a\u306e\u5206\u5b50\u30e2\u30c7\u30eb\u3067\u3082\u88fd\u54c1\u8a66\u9a13\u7d50\u679c\u3067\u3082\u3042\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n<div class=\"nhd-cp-table\">\n<table>\n<caption>\u4eee\u60f3\u7684\u306a\u308d\u904e\u524d\u5f8c\u306e\u30c7\u30fc\u30bf<\/caption>\n<thead>\n<tr>\n<th scope=\"col\">\u6bb5\u968e<\/th>\n<th scope=\"col\">\u96c6\u4e2d<\/th>\n<th scope=\"col\">\u97f3\u91cf<\/th>\n<th scope=\"col\">\u5408\u8a08<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u308d\u904e\u524d<\/td>\n<td>100 \u03bcg\/mL<\/td>\n<td>1.00 mL<\/td>\n<td>100 \u03bcg<\/td>\n<\/tr>\n<tr>\n<td>\u308d\u904e\u5f8c<\/td>\n<td>80 \u03bcg\/mL<\/td>\n<td>0.80 mL<\/td>\n<td>64 \u03bcg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<details>\n<summary>\u81ea\u5206\u3067\u5224\u65ad\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002\u6fc3\u5ea6\u3060\u3051\u3092\u898b\u305f\u5834\u5408\u300180%\u306e\u56de\u53ce\u7387\u3092\u5831\u544a\u3059\u308b\u3060\u3051\u3067\u5b8c\u5168\u3068\u8a00\u3048\u308b\u3067\u3057\u3087\u3046\u304b\uff1f<\/summary>\n<p>\u5b8c\u5168\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u91cd\u8981\u306a\u306e\u304c\u56de\u53ce\u3055\u308c\u305f\u30da\u30d7\u30c1\u30c9\u306e\u91cf\u3067\u3042\u308b\u306a\u3089\u3001\u56de\u53ce\u3055\u308c\u305f\u4f53\u7a4d\u3082\u6570\u3048\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u4eee\u5b9a\u306e\u4f8b\u3067\u306f\u3001\u7dcf\u56de\u53ce\u7387\u306f64%\u3067\u3059\u3002<\/p>\n<\/details>\n<p><a class=\"nhd-cp-download\" href=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/nhd-knowledge-20261013-worksheet-05.txt\" download>\u4e2d\u56fd\u8a9e\u306e\u30ef\u30fc\u30af\u30b7\u30fc\u30c8\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\uff08TXT\uff1b\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u30b3\u30d4\u30fc\u3057\u3066\u7de8\u96c6\uff09<\/a><\/p>\n<section class=\"nhd-cp-depth\">\n<h3>\u3059\u3079\u3066\u306e\u640d\u5931\u3092\u819c\u306b\u5272\u308a\u5f53\u3066\u308b\u306e\u306f\u907f\u3051\u308b<\/h3>\n<p>Compare a direct aliquot, an aliquot transferred without filtration, and a filtered aliquot. Record collected volumes. Knowing that 100 \u03bcg was present initially and 64 \u03bcg was recovered identifies a 36 \u03bcg shortfall; it does not allocate that amount among containers, the device, precipitate or other steps.<\/p>\n<h3>\u5b9f\u969b\u306e\u5438\u7740\u8a66\u9a13\u306f\u4f55\u3092\u793a\u3059\u306e\u304b\uff1f<\/h3>\n<p>\u30de\u30b9\u30c8\u30d1\u30e9\u30f3X\u3001\u30e1\u30ea\u30c1\u30f3\u3001\u30de\u30ac\u30a4\u30cb\u30f32\u306e2015 HPLC\u8a66\u9a13\u3067\u306f\u3001\u4e00\u90e8\u306e\u5bb9\u5668\u6761\u4ef6\u4e0b\u306790%\u4ee5\u4e0a\u306e\u640d\u5931\u304c\u5831\u544a\u3055\u308c\u305f\u3002\u3053\u306e\u8a66\u9a13\u306f\u305d\u308c\u3089\u306e\u30da\u30d7\u30c1\u30c9\u3068\u5bb9\u5668\u3092\u5bfe\u8c61\u3068\u3057\u305f\u3082\u306e\u3067\u3042\u308a\u3001\u305d\u306e\u5272\u5408\u306f\u7279\u5b9a\u306e\u30d5\u30a3\u30eb\u30bf\u30fc\u3001LL-37\u3001\u307e\u305f\u306fNHD\u88fd\u54c1\u306e\u56de\u53ce\u7387\u3067\u306f\u306a\u3044\u3002<\/p>\n<p>A useful filtration record states sample identity, processed and collected volumes, the concentration basis and the control results. \u201cGood recovery\u201d alone cannot be recalculated or applied confidently to another sample.<\/p>\n<p class=\"nhd-cp-source\"><a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0122419\" target=\"_blank\" rel=\"noopener\">\u30ac\u30e9\u30b9\u304a\u3088\u3073\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u306e\u56fa\u4f53\u8868\u9762\u3078\u306e\u967d\u30a4\u30aa\u30f3\u30da\u30d7\u30c1\u30c9\u306e\u5438\u7740<\/a> \u00b7 2015<\/p>\n<\/section>\n<h3>\u539f\u5178\u3068\u53c2\u8003\u6587\u732e<\/h3>\n<ul class=\"nhd-cp-source\">\n<li><a href=\"https:\/\/www.waters.com\/waters\/en_IE\/Peptide-Bioanalysis\/nav.htm?cid=135041966&amp;locale=en_IE\" target=\"_blank\" rel=\"noopener\">\u30da\u30d7\u30c1\u30c9\u306e\u30d0\u30a4\u30aa\u5206\u6790\u3068\u975e\u7279\u7570\u7684\u7d50\u5408<\/a><\/li>\n<\/ul>\n<p class=\"nhd-cp-source\">\u60c5\u5831\u6e90\u306f 2026-10-13 \u306b\u78ba\u8a8d\u6e08\u307f\u3067\u3059\u3002\u77e5\u898b\u306f\u7814\u7a76\u3055\u308c\u305f\u30e2\u30c7\u30eb\u3068\u6750\u6599\u306b\u9069\u7528\u3055\u308c\u307e\u3059\u3002\u30d0\u30c3\u30c1\u306e\u54c1\u8cea\u306b\u306f\u5b9f\u969b\u306e\u30d0\u30c3\u30c1\u306e\u8a18\u9332\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"<p>\u308d\u904e\u5f8c\u306e\u30b7\u30b0\u30ca\u30eb\u4f4e\u4e0b\u306f\u3001\u5fc5\u305a\u3057\u3082\u30da\u30d7\u30c1\u30c9\u304c\u3059\u3079\u3066\u819c\u306b\u5438\u7740\u3057\u305f\u3053\u3068\u3092\u610f\u5473\u3059\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u307e\u305a\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u3001\u5bb9\u91cf\u3001\u691c\u51fa\u6761\u4ef6\u3092\u63c3\u3048\u3066\u3001\u3069\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u30b5\u30f3\u30d7\u30eb\u304c\u5931\u308f\u308c\u305f\u306e\u304b\u3092\u628a\u63e1\u3057\u307e\u3057\u3087\u3046\u3002<\/p>","protected":false},"author":1,"featured_media":4528,"parent":865,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4521","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Peptide signal drops after filtration: is the filter retaining it? | NHD<\/title>\n<meta name=\"description\" content=\"A drop in signal after filtration is not necessarily all peptide adsorbed by the membrane. 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