{"id":4550,"date":"2026-10-13T16:14:10","date_gmt":"2026-10-13T16:14:10","guid":{"rendered":"https:\/\/nhdbio.com\/?page_id=4550"},"modified":"2026-10-13T16:14:10","modified_gmt":"2026-10-13T16:14:10","slug":"%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e3%81%aed%e4%bd%93%e3%83%bbl%e4%bd%93%e9%85%8d%e7%bd%ae%e8%a9%a6%e9%a8%93","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/ja\/analytical-methods\/peptide-d-l-configuration-testing\/","title":{"rendered":"\u5206\u5b50\u91cf\u306e\u4e00\u81f4\uff1a\u305d\u308c\u306f\u30da\u30d7\u30c1\u30c9\u306eD\/L\u914d\u7f6e\u3092\u78ba\u8a8d\u3059\u308b\u3082\u306e\u3067\u3059\u304b\uff1f"},"content":{"rendered":"
\n

D \u3068 L \u306f\u30a2\u30df\u30ce\u9178\u306e\u7a7a\u9593\u914d\u7f6e\u3092\u6307\u3057\u307e\u3059\u3002\u540c\u3058\u8cea\u91cf\u3092\u6301\u3064\u3053\u3068\u304c\u3042\u308b\u305f\u3081\u3001\u5206\u5b50\u91cf\u306e\u78ba\u8a8d\u3068\u30ad\u30e9\u30ea\u30c6\u30a3\u306e\u78ba\u8a8d\u306f\u5225\u3005\u306e\u554f\u3044\u3067\u3059\u3002<\/p>\n

\"\u3053\u306e\u30ac\u30a4\u30c9\u3067\u6271\u3046\u30c8\u30d4\u30c3\u30af\u306e\u30b3\u30df\u30c3\u30af\u30a4\u30e9\u30b9\u30c8\"
\u7de8\u96c6\u7528\u30a4\u30e9\u30b9\u30c8\u3067\u3042\u308a\u3001\u5b9f\u9a13\u5199\u771f\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n

D\u3068L\u306f\u3001\u91cd\u306d\u5408\u308f\u305b\u308b\u3053\u3068\u306e\u3067\u304d\u306a\u3044\u5de6\u53f3\u306e\u624b\u306e\u3088\u3046\u306a\u95a2\u4fc2\u3067\u3059<\/h3>\n

\u591a\u304f\u306e\u30a2\u30df\u30ce\u9178\u306f\u30ad\u30e9\u30eb\u3067\u3059\u3002\u7d44\u6210\u306f\u540c\u3058\u3067\u3082\u3001\u7a7a\u9593\u914d\u7f6e\u306b\u93e1\u50cf\u4f53\u304c\u5b58\u5728\u3057\u307e\u3059\u3002\u30b0\u30ea\u30b7\u30f3\u306f\u5bfe\u5fdc\u3059\u308b\u30ad\u30e9\u30eb\u4e2d\u5fc3\u3092\u6301\u305f\u306a\u3044\u305f\u3081\u3001\u4e00\u822c\u7684\u306a\u4f8b\u5916\u3067\u3059\u3002D\/L\u306e\u547d\u540d\u306f\u3001\u30da\u30d7\u30c1\u30c9\u5168\u4f53\u3092\u9006\u304b\u3089\u66f8\u304f\u3053\u3068\u3068\u5358\u7d14\u306b\u540c\u4e00\u8996\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u30da\u30d7\u30c1\u30c9\u306e\u305f\u3063\u305f1\u3064\u306e\u4f4d\u7f6e\u3067D\/L\u914d\u7f6e\u306b\u7f6e\u304d\u63db\u3048\u308b\u3053\u3068\u3068\u3001\u30da\u30d7\u30c1\u30c9\u5168\u4f53\u3092\u5b8c\u5168\u306a\u93e1\u50cf\u4f53\u306b\u5909\u3048\u308b\u3053\u3068\u306f\u3001\u5206\u6790\u5b66\u7684\u306b\u306f\u307e\u3063\u305f\u304f\u540c\u3058\u72b6\u6cc1\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

Why doesn't a mass match answer all the questions?<\/h3>\n

\u3042\u308b\u30a2\u30df\u30ce\u9178\u306eD\/L\u914d\u7f6e\u3060\u3051\u3092\u5909\u3048\u3066\u3082\u3001\u7d44\u6210\u3068\u7406\u8ad6\u8cea\u91cf\u306f\u5909\u308f\u3089\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u30a4\u30f3\u30bf\u30af\u30c8\u5206\u5b50\u8cea\u91cf\u304c\u4e00\u81f4\u3059\u308b\u3053\u3068\u3060\u3051\u3067\u306f\u3001\u76ee\u7684\u306e\u4f4d\u7f6e\u306e\u914d\u7f6e\u304c\u6b63\u3057\u3044\u3053\u3068\u3092\u8a3c\u660e\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u901a\u5e38\u306e\u975e\u30ad\u30e9\u30eb\u5206\u96e2\u3067\u305d\u308c\u3089\u3092\u533a\u5225\u3067\u304d\u308b\u304b\u3069\u3046\u304b\u306f\u3001\u7279\u5b9a\u306e\u5206\u5b50\u3068\u65b9\u6cd5\u306b\u3088\u308a\u307e\u3059\u3002\u5b8c\u5168\u306a\u93e1\u50cf\u4f53\u306e\u6750\u6599\u3068\u3001\u4e00\u90e8\u306e\u4f4d\u7f6e\u3092\u5909\u3048\u308b\u3053\u3068\u3067\u751f\u3058\u308b\u30b8\u30a2\u30b9\u30c6\u30ec\u30aa\u30de\u30fc\u306e\u6750\u6599\u3082\u5225\u3005\u306b\u8b70\u8ad6\u3059\u3079\u304d\u3067\u3059\u3002\u3059\u3079\u3066\u306e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3084\u3059\u3079\u3066\u306e\u8cea\u91cf\u5206\u6790\u304c\u5e38\u306b\u533a\u5225\u3067\u304d\u308b\u3001\u3042\u308b\u3044\u306f\u6c7a\u3057\u3066\u533a\u5225\u3067\u304d\u306a\u3044\u3068\u8a00\u3046\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u8ffd\u52a0\u306e\u8a3c\u62e0\u3092\u3069\u306e\u3088\u3046\u306b\u6c42\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u304b\uff1f<\/h3>\n

\u691c\u67fb\u6a5f\u95a2\u306b\u914d\u7f6e\u306e\u554f\u984c\u3092\u660e\u78ba\u306b\u4f1d\u3048\u3001\u9069\u5207\u306a\u65b9\u6cd5\u3068\u53c2\u7167\u6a19\u6e96\u306b\u3064\u3044\u3066\u76f8\u8ac7\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u306b\u306f\u30ad\u30e9\u30eb\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3001\u9069\u5207\u306a\u51e6\u7406\u5f8c\u306e\u30ad\u30e9\u30eb\u30a2\u30df\u30ce\u9178\u5206\u6790\u3001\u307e\u305f\u306f\u7279\u5b9a\u306e\u6750\u6599\u3067\u691c\u8a3c\u3055\u308c\u305f\u305d\u306e\u4ed6\u306e\u65b9\u6cd5\u304c\u542b\u307e\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u4e00\u90e8\u306e\u7814\u7a76\u3067\u306f\u3001D-\u30a2\u30df\u30ce\u9178\u3092\u542b\u3080\u30b8\u30a2\u30b9\u30c6\u30ec\u30aa\u30de\u30fc\u30da\u30d7\u30c1\u30c9\u3092\u533a\u5225\u3059\u308b\u305f\u3081\u306b\u7279\u5b9a\u306e\u30bf\u30f3\u30c7\u30e0\u8cea\u91cf\u5206\u6790\u6761\u4ef6\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u7279\u5b9a\u306e\u65b9\u6cd5\u304c\u60c5\u5831\u3092\u63d0\u4f9b\u3067\u304d\u308b\u3053\u3068\u3092\u793a\u3059\u3082\u306e\u3067\u3042\u308a\u3001\u65e5\u5e38\u7684\u306aLC-MS\u8cea\u91cf\u5206\u6790\u7d50\u679c\u304c\u3059\u3079\u3066\u306e\u30ad\u30e9\u30eb\u78ba\u8a8d\u3092\u3059\u3067\u306b\u5b8c\u4e86\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3059\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

\u51e6\u7406\u30d7\u30ed\u30bb\u30b9\u81ea\u4f53\u3082\u78ba\u8a8d\u306b\u542b\u3081\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059<\/h3>\n

\u65b9\u6cd5\u304c\u52a0\u6c34\u5206\u89e3\u307e\u305f\u306f\u305d\u306e\u4ed6\u306e\u51e6\u7406\u3092\u5fc5\u8981\u3068\u3059\u308b\u5834\u5408\u3001\u305d\u306e\u51e6\u7406\u304c\u7acb\u4f53\u914d\u7f6e\u306e\u5909\u5316\u3092\u5f15\u304d\u8d77\u3053\u3059\u304b\u3069\u3046\u304b\u3082\u8a55\u4fa1\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u6700\u7d42\u7684\u306a\u65b9\u6cd5\u306f\u3001\u51e6\u7406\u3055\u308c\u305f\u751f\u6210\u7269\u306e\u3042\u308b\u7279\u6027\u3092\u6e2c\u5b9a\u3059\u308b\u3060\u3051\u3067\u306a\u304f\u3001\u5143\u306e\u6750\u6599\u306b\u95a2\u3059\u308b\u554f\u3044\u306b\u7b54\u3048\u3089\u308c\u308b\u3082\u306e\u3067\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002<\/p>\n

When ordering, write the D positions, terminal modifications, and full sequence clearly. Labeling only "D peptide" can easily omit which positions need to be D and whether reverse order is also required.<\/p>\n

\u6b63\u78ba\u306a\u8a66\u9a13\u4f9d\u983c\u306f\u3069\u306e\u3088\u3046\u306b\u66f8\u304f\u3079\u304d\u3067\u3059\u304b\uff1f<\/h3>\n

You can write: We wish to confirm the D\/L configuration at specific positions in the given sequence; please state the analytical evidence that can be provided, the methods used, reference standards, and method limitations. This is closer to the identity that actually needs to be confirmed than "a correct molecular weight is enough."<\/p>\n

3\u3064\u306e\u8cea\u554f\u30013\u7a2e\u985e\u306e\u8a3c\u62e0\u306e\u5fc5\u8981\u6027<\/figcaption>
\n
\u8cea\u91cf\u304c\u4e88\u60f3\u3055\u308c\u308b\u7d44\u6210\u3068\u4e00\u81f4\u3059\u308b\u304b\u3069\u3046\u304b<\/b>\u5b8c\u5168\u306a\u5206\u5b50\u91cf\u3060\u3051\u3067\u5341\u5206\u304b\uff1a\u5b8c\u5168\u306a\u5206\u5b50\u91cf\u306f\u60c5\u5831\u3092\u63d0\u4f9b\u3067\u304d\u308b\u304c\u3001\u4ed6\u306e\u540c\u4e00\u6027\u8a18\u9332\u3068\u7d44\u307f\u5408\u308f\u305b\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059<\/span><\/div>\n
\u30a2\u30df\u30ce\u9178\u914d\u5217\u304c\u6b63\u3057\u3044\u304b\u3069\u3046\u304b<\/b>\u5b8c\u5168\u306a\u5206\u5b50\u91cf\u3060\u3051\u3067\u5341\u5206\u304b\uff1a\u901a\u5e38\u3001\u3055\u3089\u306a\u308b\u914d\u5217\u306e\u8a3c\u62e0\u304c\u5fc5\u8981\u3067\u3059<\/span><\/div>\n
\u6307\u5b9a\u3055\u308c\u305f\u4f4d\u7f6e\u304cD\u3067\u3042\u308b\u304b\u3069\u3046\u304b<\/b>\u5b8c\u5168\u306a\u5206\u5b50\u91cf\u3060\u3051\u3067\u5341\u5206\u304b\uff1a\u7acb\u4f53\u914d\u7f6e\u306e\u554f\u3044\u306b\u5bfe\u5fdc\u3059\u308b\u9069\u7528\u53ef\u80fd\u306a\u8a3c\u62e0\u304c\u5fc5\u8981\u3067\u3059<\/span><\/div>\n<\/div>
\u6559\u80b2\u7528\u307e\u3068\u3081\u3002\u8aac\u660e\u3068\u539f\u5178\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u306f\u7e2e\u5c3a\u901a\u308a\u306e\u5206\u5b50\u30e2\u30c7\u30eb\u3067\u3082\u88fd\u54c1\u8a66\u9a13\u7d50\u679c\u3067\u3082\u3042\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n
\n\n\n\n\n\n\n\n
3\u3064\u306e\u8cea\u554f\u30013\u7a2e\u985e\u306e\u8a3c\u62e0\u306e\u5fc5\u8981\u6027<\/caption>\n
\u8cea\u554f<\/th>\n\u5b8c\u5168\u306a\u5206\u5b50\u91cf\u3060\u3051\u3067\u5341\u5206\u304b<\/th>\n<\/tr>\n<\/thead>\n
\u8cea\u91cf\u304c\u4e88\u60f3\u3055\u308c\u308b\u7d44\u6210\u3068\u4e00\u81f4\u3059\u308b\u304b\u3069\u3046\u304b<\/td>\n\u5b8c\u5168\u306a\u5206\u5b50\u91cf\u306f\u60c5\u5831\u3092\u63d0\u4f9b\u3067\u304d\u308b\u304c\u3001\u4ed6\u306e\u540c\u4e00\u6027\u8a18\u9332\u3068\u7d44\u307f\u5408\u308f\u305b\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059<\/td>\n<\/tr>\n
\u30a2\u30df\u30ce\u9178\u914d\u5217\u304c\u6b63\u3057\u3044\u304b\u3069\u3046\u304b<\/td>\n\u901a\u5e38\u3001\u3055\u3089\u306a\u308b\u914d\u5217\u306e\u8a3c\u62e0\u304c\u5fc5\u8981\u3067\u3059<\/td>\n<\/tr>\n
\u6307\u5b9a\u3055\u308c\u305f\u4f4d\u7f6e\u304cD\u3067\u3042\u308b\u304b\u3069\u3046\u304b<\/td>\n\u69cb\u6210\u306e\u7591\u554f\u306b\u5bfe\u51e6\u3059\u308b\u9069\u7528\u53ef\u80fd\u306a\u30a8\u30d3\u30c7\u30f3\u30b9\u304c\u5fc5\u8981\u3067\u3059<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\n

\u5143\u306e\u65b9\u6cd5\u7814\u7a76\u306f\u4f55\u3092\u884c\u3044\u307e\u3057\u305f\u304b\uff1f<\/h3>\n

Adams and Zubarev compared ECD and CAD fragmentation in 2005 using models including tryptophan cage, a lactoferrin peptide and dermorphin. Their abstract reports calibration accuracy of 1% for ECD and 3\u20135% for CAD for relative D-residue abundance in the studied diastereomer mixtures. These are study-specific calibration results, not routine LC-MS detection limits or guaranteed errors.<\/p>\n

\u8cea\u91cf\u78ba\u8a8d\u3068\u7acb\u4f53\u5316\u5b66\u7684\u78ba\u8a8d\u3092\u5225\u3005\u306b\u4f9d\u983c\u3057\u3066\u304f\u3060\u3055\u3044<\/h3>\n

A mass match means the measured intact mass is compatible with the target record. Confirming configuration needs a method and controls that distinguish the relevant stereoisomers. If the report shows only an intact-mass peak, mark configuration as \u201cnot established by this report\u201d rather than assuming it passed.<\/p>\n<\/section>\n

\u539f\u5178\u3068\u53c2\u8003\u6587\u732e<\/h3>\n