{"id":871,"date":"2026-09-02T06:35:32","date_gmt":"2026-09-02T06:35:32","guid":{"rendered":"https:\/\/nhdbio.com\/analytical-methods\/chromatogram-interpretation\/"},"modified":"2026-10-10T09:19:22","modified_gmt":"2026-10-10T09:19:22","slug":"%e3%82%af%e3%83%ad%e3%83%9e%e3%83%88%e3%82%b0%e3%83%a9%e3%83%a0%e3%81%ae%e8%a7%a3%e9%87%88","status":"publish","type":"page","link":"https:\/\/nhdbio.com\/ja\/analytical-methods\/chromatogram-interpretation\/","title":{"rendered":"\u30da\u30d7\u30c1\u30c9\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\u306e\u8aad\u307f\u65b9\uff1a95%\u7d14\u5ea6\u3092\u7406\u89e3\u3059\u308b"},"content":{"rendered":"<div class=\"nhd-teaching-body\">\n<p>Open a peptide test report and you may see a curve that looks like a range of mountains, with \u201c95% purity\u201d printed beside it. Where does that number come from? Is the tallest peak necessarily the peptide you ordered?<\/p>\n<p>\u3053\u306e\u30ac\u30a4\u30c9\u3092\u7406\u89e3\u3059\u308b\u306e\u306b\u5316\u5b66\u306e\u80cc\u666f\u306f\u5fc5\u8981\u3042\u308a\u307e\u305b\u3093\u30021\u3064\u306e\u4f8b\u306e\u30c8\u30ec\u30fc\u30b9\u3092\u8aad\u307f\u3001\u305d\u306e\u9762\u7a4d\u7d14\u5ea6\u3092\u8a08\u7b97\u3057\u3001\u5831\u544a\u66f8\u304c\u7b54\u3048\u308b\u3079\u304d\u8cea\u554f\u3092\u7279\u5b9a\u3057\u307e\u3059\u3002<\/p>\n<div class=\"note\"><strong>\u3053\u306e\u8a18\u4e8b\u3092\u8aad\u307f\u7d42\u3048\u308b\u3068\u3001\u6b21\u306e\u3053\u3068\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\uff1a<\/strong> \u4fdd\u6301\u6642\u9593\u3068\u30d4\u30fc\u30af\u9762\u7a4d\u3092\u7279\u5b9a\u3057\u3001\u7d14\u5ea6\u8a08\u7b97\u3092\u78ba\u8a8d\u3057\u3001\u6e2c\u5b9a\u304c\u793a\u3059\u3082\u306e\u3068\u793a\u305b\u306a\u3044\u3082\u306e\u3092\u533a\u5225\u3059\u308b\u3002\u4ee5\u4e0b\u306e\u56f3\u3068\u6570\u5024\u306f\u3059\u3079\u3066\u6a21\u64ec\u7684\u306a\u6559\u80b2\u4f8b\u3067\u3042\u308a\u3001NHD\u306e\u30d0\u30c3\u30c1\u8a66\u9a13\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/div>\n<h2>1\u3002\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\u306f\u4f55\u3092\u8a18\u9332\u3059\u308b\u306e\u304b\uff1f<\/h2>\n<p>HPLC\u306f\u9ad8\u901f\u6db2\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3092\u610f\u5473\u3057\u307e\u3059\u3002\u6df7\u5408\u7269\u4e2d\u306e\u6210\u5206\u3092\u5206\u96e2\u3057\u3001\u305d\u308c\u3089\u304c\u88c5\u7f6e\u3092\u901a\u904e\u3059\u308b\u969b\u306b\u691c\u51fa\u3059\u308b\u65b9\u6cd5\u3068\u8003\u3048\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u7570\u306a\u308b\u6210\u5206\u306f\u30ab\u30e9\u30e0\u3092\u7570\u306a\u308b\u901f\u5ea6\u3067\u79fb\u52d5\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u308c\u3089\u304c\u691c\u51fa\u5668\u306b\u5230\u9054\u3059\u308b\u3068\u3001\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u304c\u305d\u306e\u4fe1\u53f7\u3092\u6642\u9593\u3068\u3068\u3082\u306b\u30d7\u30ed\u30c3\u30c8\u3057\u307e\u3059\u3002\u305d\u306e\u30d7\u30ed\u30c3\u30c8\u304c\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\u3067\u3059\u3002\u305d\u306e <a href=\"https:\/\/www.waters.com\/nextgen\/us\/en\/education\/primers\/beginner-s-guide-to-uplc\/bands-peaks-and-band-spreading.html\">Waters beginner\u2019s guide<\/a> \u5206\u96e2\u3068\u3001\u305d\u306e\u7d50\u679c\u751f\u3058\u308b\u30d4\u30fc\u30af\u3068\u306e\u95a2\u4fc2\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n<p>\u307e\u305a\u56f3 1 \u306e\u8ef8\u30684\u3064\u306e\u30d4\u30fc\u30af\u304b\u3089\u898b\u3066\u3044\u304d\u307e\u3057\u3087\u3046\u3002\u307e\u3060\u3059\u3079\u3066\u306e\u6570\u5024\u3092\u7406\u89e3\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<figure><img src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/lesson-images\/en\/01-read-chromatogram.png\" loading=\"lazy\" decoding=\"async\" alt=\"\u6642\u9593\u8ef8\u3068\u691c\u51fa\u5668\u5fdc\u7b54\u8ef8\u3092\u5099\u3048\u305f\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\u3002\u30d4\u30fc\u30afB\u306f\u76ee\u7684\u7269\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u305f\u3068\u4eee\u5b9a\"><figcaption>\u56f3 1\u3002B\u304c\u76ee\u7684\u6210\u5206\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u305f\u3068\u4eee\u5b9a\u3057\u3066\u3044\u307e\u3059\u3002\u6642\u9593\u3001\u5fdc\u7b54\u3001\u30d4\u30fc\u30af\u5f62\u72b6\u306f\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3067\u3059\u3002<\/figcaption><\/figure>\n<ul>\n<li><strong>\u6a2a\u8ef8\uff1a\u6642\u9593\u3002<\/strong> \u3053\u3053\u3067\u3001min\u306f\u5206\u3092\u610f\u5473\u3057\u307e\u3059\u3002\u3053\u306e\u8a66\u9a13\u6761\u4ef6\u3067\u306f\u3001B\u306f 6.2 \u5206\u3067\u6700\u5927\u30b7\u30b0\u30ca\u30eb\u306b\u9054\u3057\u307e\u3059\u3002\u3053\u308c\u304cB\u306e\u4fdd\u6301\u6642\u9593\u3067\u3059\u3002<\/li>\n<li><strong>\u7e26\u8ef8\uff1a\u691c\u51fa\u5668\u5fdc\u7b54\u3002<\/strong> \u3053\u308c\u306f\u30b7\u30b0\u30ca\u30eb\u306e\u5f37\u3055\u3092\u793a\u3059\u3082\u306e\u3067\u3001\u30d0\u30a4\u30a2\u30eb\u4e2d\u306e\u30df\u30ea\u30b0\u30e9\u30e0\u6570\u3092\u76f4\u63a5\u793a\u3059\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u5b9f\u969b\u306eUV\u30ec\u30dd\u30fc\u30c8\u3067\u306fmAU\u304c\u4f7f\u308f\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u5b9f\u969b\u306e\u30ec\u30dd\u30fc\u30c8\u306b\u8a18\u8f09\u3055\u308c\u305f\u5358\u4f4d\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li><strong>\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\uff1a\u30d4\u30fc\u30af\u306e\u4e0b\u306b\u3042\u308b\u57fa\u6e96\u7dda\u3002<\/strong> \u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u304c\u5404\u30d4\u30fc\u30af\u306e\u9762\u7a4d\u3092\u8a08\u7b97\u3059\u308b\u306b\u306f\u3001\u958b\u59cb\u70b9\u3001\u7d42\u4e86\u70b9\u3001\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u304c\u5fc5\u8981\u3067\u3059\u3002<\/li>\n<\/ul>\n<p><strong>\u6700\u521d\u306e\u78ba\u8a8d\uff1a<\/strong> \u691c\u51fa\u5668\u30c1\u30e3\u30f3\u30cd\u30eb\u3068\u5358\u4f4d\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u7570\u306a\u308b\u691c\u51fa\u5668\u3084\u6ce2\u9577\u306e\u30d4\u30fc\u30af\u9ad8\u3055\u3092\u76f4\u63a5\u6bd4\u8f03\u3057\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>2\u3002\u9762\u7a4d\u7d14\u5ea6\u306f\u9ad8\u3055\u3067\u306f\u306a\u304f\u9762\u7a4d\u306b\u4f9d\u5b58\u3057\u307e\u3059<\/h2>\n<p>\u30d4\u30fc\u30af\u3092\u5c71\u306b\u305f\u3068\u3048\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u5c71\u9802\u306f\u9ad8\u3055\u3092\u8868\u3057\u3001\u30d9\u30fc\u30b9\u30e9\u30a4\u30f3\u3088\u308a\u4e0a\u306e\u7a7a\u9593\u306f\u9762\u7a4d\u3092\u8868\u3057\u307e\u3059\u3002\u7d30\u3044\u30d4\u30fc\u30af\u306f\u3001\u9762\u7a4d\u304c\u5927\u304d\u304f\u306a\u304f\u3066\u3082\u9ad8\u304f\u898b\u3048\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<figure><img src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/lesson-images\/en\/02-height-vs-area.png\" loading=\"lazy\" decoding=\"async\" alt=\"\u9762\u7a4d\u304c\u7b49\u3057\u30442\u3064\u306e\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u30d4\u30fc\u30af100\uff1a\u4e00\u65b9\u306f\u72ed\u304f\u9ad8\u304f\u3001\u3082\u3046\u4e00\u65b9\u306f\u5e83\u304f\u4f4e\u3044\"><figcaption>\u56f3 2\u3002\u4e21\u65b9\u306e\u30d4\u30fc\u30af\u306e\u9762\u7a4d\u306f 100 \u3067\u3059\u3002\u9ad8\u3044\u307b\u3046\u304c\u9ad8\u3044\u7d14\u5ea6\u3092\u793a\u3059\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n<p>HPLC \u306e\u9762\u7a4d\u7d14\u5ea6\u306f\u3001\u901a\u5e38\u3001\u5bfe\u8c61\u30d4\u30fc\u30af\u306e\u9762\u7a4d\u3092\u8a08\u7b97\u306b\u542b\u307e\u308c\u308b\u7dcf\u9762\u7a4d\u3068\u6bd4\u8f03\u3057\u307e\u3059\u3002 <a href=\"https:\/\/www.bachem.com\/knowledge-center\/quality-control-of-amino-acids-peptides-a-guide\/\">Bachem\u2019s peptide quality-control guide<\/a> describes this approach and UV assessment around 210\u2013220 nm. Check the wavelength and method used in your own report.<\/p>\n<p>\u56f31\u306e\u7a4d\u5206\u8868\u306b\u3053\u308c\u3089\u306e\u7d50\u679c\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u3068\u3057\u307e\u3059\u30024\u3064\u306e\u30d4\u30fc\u30af\u304c\u3059\u3079\u3066\u542b\u307e\u308c\u3066\u304a\u308a\u3001\u3053\u306e\u4f8b\u3067\u306f\u30ec\u30b9\u30dd\u30f3\u30b9\u30d5\u30a1\u30af\u30bf\u30fc\u88dc\u6b63\u306f\u9069\u7528\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n<table>\n<thead>\n<tr>\n<th>\u30d4\u30fc\u30af<\/th>\n<th>\u4fdd\u6301\u6642\u9593<\/th>\n<th>\u7a4d\u5206\u9762\u7a4d\uff08\u4efb\u610f\u5358\u4f4d\uff09<\/th>\n<th>\u9762\u7a4d\u5272\u5408<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>A<\/td>\n<td>3.1\u5206<\/td>\n<td>25<\/td>\n<td>2.5%<\/td>\n<\/tr>\n<tr>\n<td><strong>B\uff1a\u30bf\u30fc\u30b2\u30c3\u30c8<\/strong><\/td>\n<td>6.2 \u5206<\/td>\n<td><strong>950<\/strong><\/td>\n<td><strong>95.0%<\/strong><\/td>\n<\/tr>\n<tr>\n<td>C<\/td>\n<td>8.4 \u5206<\/td>\n<td>15<\/td>\n<td>1.5%<\/td>\n<\/tr>\n<tr>\n<td>D<\/td>\n<td>10.1 \u5206<\/td>\n<td>10<\/td>\n<td>1.0%<\/td>\n<\/tr>\n<tr>\n<td>\u5408\u8a08<\/td>\n<td>\u2014<\/td>\n<td>1,000<\/td>\n<td>100%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u307e\u305a\u3001\u542b\u307e\u308c\u3066\u3044\u308b\u9762\u7a4d\u3092\u5408\u8a08\u3057\u307e\u3059\uff1a25 + 950 + 15 + 10 = 1,000\u3002\u6b21\u306b\u3001\u76ee\u6a19\u9762\u7a4d\u3092\u305d\u306e\u5408\u8a08\u3067\u5272\u308a\u307e\u3059\uff1a<\/p>\n<p class=\"formula\">950 \u00f7 1,000 \u00d7 100% = 95.0%<\/p>\n<p>\u3053\u306e\u7d50\u679c\u306f\u3001\u6b21\u306e\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3059\uff1a <strong>\u30bf\u30fc\u30b2\u30c3\u30c8\u306f\u3001\u542b\u307e\u308c\u308b\u30d4\u30fc\u30af\u9762\u7a4d\u306e95.0%\u3092\u5360\u3081\u3066\u3044\u307e\u3059\u3002<\/strong> \u5b9f\u969b\u306e\u30ec\u30dd\u30fc\u30c8\u3092\u78ba\u8a8d\u3059\u308b\u3068\u304d\u306f\u3001\u305d\u306e\u7a4d\u5206\u8868\u3068\u8a08\u7b97\u30eb\u30fc\u30eb\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<div class=\"exercise\"><strong>\u8a66\u3057\u3066\u307f\u307e\u3057\u3087\u3046\uff1a<\/strong> \u30bf\u30fc\u30b2\u30c3\u30c8\u9762\u7a4d\u306f980\u3067\u3001\u4ed6\u306b\u542b\u307e\u308c\u308b2\u3064\u306e\u30d4\u30fc\u30af\u306e\u9762\u7a4d\u306f12\u30688\u3067\u3059\u3002\u9762\u7a4d\u7d14\u5ea6\u306f\u3044\u304f\u3089\u3067\u3059\u304b\uff1f<\/p>\n<details>\n<summary>\u7b54\u3048\u3092\u8868\u793a<\/summary>\n<p>980 \u00f7 (980 + 12 + 8) \u00d7 100% = 98.0%.<\/p>\n<\/details>\n<\/div>\n<h2>3. A peak\u2019s position does not prove its identity<\/h2>\n<p>\u56f31\u3067\u306f\u3001B\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u3067\u3042\u308b\u3068\u3059\u3067\u306b\u4eee\u5b9a\u3057\u3066\u3044\u307e\u3059\u3002B\u304c\u6700\u5927\u306e\u30d4\u30fc\u30af\u3060\u304b\u3089\u3068\u3044\u3046\u3060\u3051\u3067\u3001\u305d\u306e\u4eee\u5b9a\u3092\u3057\u3066\u306f\u3044\u3051\u307e\u305b\u3093\u3002<\/p>\n<p>\u4fdd\u6301\u6642\u9593\u306f\u30e1\u30bd\u30c3\u30c9\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002\u30ab\u30e9\u30e0\u3001\u79fb\u52d5\u76f8\u3001\u307e\u305f\u306f\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u304c\u5909\u308f\u308b\u3068\u3001\u6210\u5206\u304c\u5225\u306e\u6642\u9593\u306b\u73fe\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u7570\u306a\u308b\u6210\u5206\u3067\u3082\u4fdd\u6301\u6642\u9593\u304c\u985e\u4f3c\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u5bfe\u8c61\u30d4\u30fc\u30af\u304c\u3069\u306e\u3088\u3046\u306b\u540c\u5b9a\u3055\u308c\u305f\u304b\u3092\u5c0b\u306d\u3066\u304f\u3060\u3055\u3044\u3002\u8cea\u554f\u306b\u3088\u3063\u3066\u306f\u3001\u53c2\u7167\u7269\u8cea\u3001\u8cea\u91cf\u6e2c\u5b9a\u3001\u307e\u305f\u306f\u305d\u306e\u4ed6\u306e\u9069\u5207\u306a\u8a3c\u62e0\u304c\u540c\u5b9a\u3092\u652f\u6301\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002HPLC \u5206\u96e2\u3068\u8cea\u91cf\u5206\u6790\u306b\u3088\u308b\u8a3c\u62e0\u306f\u7570\u306a\u308b\u554f\u3044\u306b\u7b54\u3048\u308b\u3082\u306e\u3067\u3042\u308a\u3001\u3069\u3061\u3089\u3082\u4ed6\u65b9\u306e\u4e07\u80fd\u306a\u4ee3\u66ff\u3068\u3057\u3066\u6271\u3046\u3079\u304d\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<h2>4\u3002\u5c0b\u306d\u308b\u4fa1\u5024\u306e\u3042\u308b3\u3064\u306e\u30d1\u30bf\u30fc\u30f3<\/h2>\n<figure><img src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/lesson-images\/en\/03-three-patterns.png\" loading=\"lazy\" decoding=\"async\" alt=\"\u5206\u96e2\u3057\u305f\u30d4\u30fc\u30af\u3001\u30b7\u30e7\u30eb\u30c0\u30fc\u30d4\u30fc\u30af\u3001\u8a66\u6599\u3068\u30d6\u30e9\u30f3\u30af\u306e\u4e21\u65b9\u306b\u73fe\u308c\u308b\u30b7\u30b0\u30ca\u30eb\u306e\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u4f8b\"><figcaption>\u56f3 3\u3002\u3053\u308c\u3089\u306e\u30d1\u30bf\u30fc\u30f3\u306f\u7570\u306a\u308b\u8ffd\u52a0\u78ba\u8a8d\u306e\u554f\u3044\u3092\u5fc5\u8981\u3068\u3057\u307e\u3059\u3002\u305d\u308c\u3089\u306f\u4e07\u80fd\u306a\u5408\u5426\u57fa\u6e96\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n<ul>\n<li><strong>\u4e92\u3044\u306b\u5206\u96e2\u3057\u305f\u30d4\u30fc\u30af\uff1a<\/strong> their areas are generally easier to integrate separately. A small peak\u2019s identity still cannot be inferred from its position alone.<\/li>\n<li><strong>\u30e1\u30a4\u30f3\u30d4\u30fc\u30af\u306e\u6a2a\u306e\u5c0f\u3055\u306a\u30b7\u30e7\u30eb\u30c0\u30fc\uff1a<\/strong> \u3053\u308c\u306f\u4e0d\u5b8c\u5168\u306a\u5206\u96e2\u3092\u793a\u5506\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u4ed6\u306e\u8981\u56e0\u3082\u5bc4\u4e0e\u3057\u5f97\u307e\u3059\u3002\u305d\u308c\u304c\u5bfe\u8c61\u9762\u7a4d\u306e\u8a08\u7b97\u306b\u5f71\u97ff\u3059\u308b\u304b\u3069\u3046\u304b\u3092\u5c0b\u306d\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li><strong>\u30d6\u30e9\u30f3\u30af\u4e2d\u306e\u985e\u4f3c\u30d4\u30fc\u30af\uff1a<\/strong> \u6eb6\u5a92\u3084\u30b7\u30b9\u30c6\u30e0\u306e\u5bc4\u4e0e\uff08\u30ad\u30e3\u30ea\u30fc\u30aa\u30fc\u30d0\u30fc\u306e\u53ef\u80fd\u6027\u3092\u542b\u3080\uff09\u3092\u8abf\u3079\u3066\u304f\u3060\u3055\u3044\u3002\u30da\u30d7\u30c1\u30c9\u4e0d\u7d14\u7269\u3068\u81ea\u52d5\u7684\u306b\u6c7a\u3081\u3064\u3051\u305f\u308a\u3001\u8aac\u660e\u306a\u3057\u306b\u9664\u53bb\u3057\u305f\u308a\u3057\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<p>\u30d4\u30fc\u30af\u306e\u9577\u3044\u30c6\u30fc\u30ea\u30f3\u30b0\u3082\u8b70\u8ad6\u3059\u308b\u4fa1\u5024\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u305d\u308c\u3060\u3051\u3067\u88fd\u54c1\u304c\u5206\u89e3\u3057\u305f\u8a3c\u62e0\u306b\u306f\u306a\u308a\u307e\u305b\u3093\u3002\u8a66\u9a13\u6761\u4ef6\u3001\u6ce8\u5165\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u3044\u305a\u308c\u3082\u30d4\u30fc\u30af\u5f62\u72b6\u306b\u5f71\u97ff\u3057\u5f97\u307e\u3059\u3002<\/p>\n<p>\u898b\u843d\u3068\u3055\u308c\u3084\u3059\u3044\u3082\u3046\u4e00\u3064\u306e\u9650\u754c\uff1a <strong>2\u3064\u306e\u6210\u5206\u304c\u540c\u3058\u898b\u304b\u3051\u306e\u30d4\u30fc\u30af\u306b\u5bc4\u4e0e\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/strong> \u30d4\u30fc\u30af\u304c1\u3064\u898b\u3048\u308b\u3053\u3068\u306f\u3001\u6210\u5206\u304c1\u3064\u3060\u3051\u5b58\u5728\u3059\u308b\u8a3c\u62e0\u306b\u306f\u306a\u308a\u307e\u305b\u3093\u3002<\/p>\n<h2>5\u3002\u5c0f\u3055\u306a\u30d4\u30fc\u30af\u30921\u3064\u9664\u5916\u3059\u308b\u3068\u5272\u5408\u304c\u5909\u308f\u308a\u307e\u3059<\/h2>\n<p>Integration is the software\u2019s process of setting the baseline and peak boundaries, then calculating areas. Which signals are included affects the result. <a href=\"https:\/\/www.agilent.com\/about\/data-integrity\/en\/5-tips-chromatographic-integration.html\">Agilent\u2019s integration guidance<\/a> \u30d4\u30fc\u30af\u5e45\u3001\u3057\u304d\u3044\u5024\u3001\u51e6\u7406\u65b9\u6cd5\u306a\u3069\u306e\u8a2d\u5b9a\u304c\u306a\u305c\u5206\u6790\u624b\u9806\u306b\u5f93\u3046\u5fc5\u8981\u304c\u3042\u308a\u3001\u30ec\u30d3\u30e5\u30fc\u3055\u308c\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u304b\u3092\u8aac\u660e\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306e\u4f8b\u306b\u623b\u308a\u307e\u3057\u3087\u3046\u3002\u9762\u7a4d 25 \u306eA\u304c\u9664\u5916\u3055\u308c\u305f\u5834\u5408\u3001\u5408\u8a08\u306f 1,000 \u3067\u306f\u306a\u304f 975 \u306b\u306a\u308a\u307e\u3059\uff1a<\/p>\n<p class=\"formula\">950 \u00f7 975 \u00d7 100% \u2248 97.44%<\/p>\n<p>\u8a66\u6599\u306f\u5909\u308f\u3063\u3066\u3044\u307e\u305b\u3093\u304c\u3001\u8a08\u7b97\u7d50\u679c\u306f 95.0% \u3067\u306f\u306a\u304f\u7d04 97.44% \u3068\u306a\u308a\u307e\u3059\u3002\u9664\u5916\u304c\u5e38\u306b\u8aa4\u308a\u3068\u306f\u9650\u308a\u307e\u305b\u3093\u3002\u65b9\u6cd5\u306b\u57fa\u3065\u3044\u3066\u6b63\u5f53\u306b\u9664\u5916\u3067\u304d\u308b\u30b7\u30b0\u30ca\u30eb\u3082\u3042\u308a\u307e\u3059\u3002\u91cd\u8981\u306a\u554f\u3044\u306f\u3001 <strong>\u4f55\u304c\u9664\u5916\u3055\u308c\u3001\u306a\u305c\u9664\u5916\u3055\u308c\u305f\u306e\u304b\uff1f<\/strong> \u5370\u5237\u3055\u308c\u305f\u7d50\u679c\u3092\u826f\u304f\u898b\u305b\u308b\u305f\u3081\u3060\u3051\u306b\u30d4\u30fc\u30af\u3092\u9664\u53bb\u3059\u3079\u304d\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<h2>6. \u201c95% area purity\u201d does not mean 9.5 mg of target peptide in 10 mg of powder<\/h2>\n<p>Area purity compares signals detected and included under a particular method. The powder\u2019s weight may also include water, counterions or other components.<\/p>\n<p>\u3057\u305f\u304c\u3063\u3066\u300110 mg \u8868\u793a\u3068 95% HPLC \u9762\u7a4d\u7d14\u5ea6\u7d50\u679c\u3060\u3051\u3067\u306f\u3001\u30d0\u30a4\u30a2\u30eb\u306b\u76ee\u6a19\u30da\u30d7\u30c1\u30c9\u306e 9.5 mg \u304c\u542b\u307e\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u7acb\u3067\u304d\u307e\u305b\u3093\u3002\u5b9f\u9a13\u3067\u5b9a\u7fa9\u3055\u308c\u305f\u30da\u30d7\u30c1\u30c9\u91cf\u304c\u5fc5\u8981\u306a\u5834\u5408\u306f\u3001\u9069\u5207\u306a\u542b\u91cf\u6e2c\u5b9a\u3068\u305d\u306e\u5831\u544a\u57fa\u6e96\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002 <a href=\"https:\/\/www.bachem.com\/knowledge-center\/quality-control-of-amino-acids-peptides-a-guide\/\">Bachem\u2019s guide<\/a> \u306f\u7d14\u5ea6\u3068\u30da\u30d7\u30c1\u30c9\u542b\u91cf\u3092\u533a\u5225\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>HPLC \u306e\u9762\u7a4d\u7d14\u5ea6\u306f\u3001\u30a8\u30f3\u30c9\u30c8\u30ad\u30b7\u30f3\u3001\u7121\u83cc\u6027\u3001\u305d\u306e\u4ed6\u306e\u5c02\u7528\u6e2c\u5b9a\u306b\u4ee3\u308f\u308b\u3082\u306e\u3067\u3082\u3042\u308a\u307e\u305b\u3093\u30021\u3064\u306e\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3067\u3059\u3079\u3066\u306e\u54c1\u8cea\u4e0a\u306e\u7591\u554f\u306b\u7b54\u3048\u3089\u308c\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<h2>\u5831\u544a\u66f8\u3092\u8aad\u3080\u305f\u3081\u306e\u5b9f\u8df5\u7684\u306a\u9806\u5e8f<\/h2>\n<ol>\n<li><strong>\u30b5\u30f3\u30d7\u30eb\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\uff1a<\/strong> \u540d\u524d\u3001\u4ed5\u69d8\u3001\u30ed\u30c3\u30c8\u756a\u53f7\u306f\u3001\u78ba\u8a8d\u3057\u3066\u3044\u308b\u6750\u6599\u3068\u4e00\u81f4\u3057\u3066\u3044\u307e\u3059\u304b\uff1f<\/li>\n<li><strong>\u65b9\u6cd5\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\uff1a<\/strong> \u691c\u51fa\u5668\u30c1\u30e3\u30f3\u30cd\u30eb\u3001\u6ce2\u9577\u3001\u30ab\u30e9\u30e0\u3001\u307e\u305f\u306f\u30e1\u30bd\u30c3\u30c9\u306e\u53c2\u7167\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u304b\uff1f<\/li>\n<li><strong>\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u7279\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\uff1a<\/strong> \u30d4\u30fc\u30af\u306f\u540c\u5b9a\u3055\u308c\u3066\u3044\u307e\u3059\u304b\uff1f\u307e\u305f\u3001\u305d\u306e\u540c\u5b9a\u3092\u88cf\u4ed8\u3051\u308b\u3082\u306e\u306f\u4f55\u3067\u3059\u304b\uff1f<\/li>\n<li><strong>\u9762\u7a4d\u30c6\u30fc\u30d6\u30eb\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\uff1a<\/strong> \u30bf\u30fc\u30b2\u30c3\u30c8\u9762\u7a4d\u3068\u5408\u8a08\u9762\u7a4d\u306f\u3001\u5831\u544a\u3055\u308c\u305f\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3092\u518d\u73fe\u3057\u3066\u3044\u307e\u3059\u304b\uff1f<\/li>\n<li><strong>\u8aac\u660e\u3055\u308c\u3066\u3044\u306a\u3044\u7279\u5fb4\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\uff1a<\/strong> \u660e\u78ba\u5316\u304c\u5fc5\u8981\u306a\u30b7\u30e7\u30eb\u30c0\u30fc\u3001\u5c0f\u3055\u306a\u30d4\u30fc\u30af\u3001\u9664\u5916\u306f\u3042\u308a\u307e\u3059\u304b\uff1f<\/li>\n<\/ol>\n<p>You can now ask specific questions rather than judging whether a trace \u201clooks good\u201d: a mismatched lot number, an unassigned target peak, a missing area table or an unclear calculation basis.<\/p>\n<h2>\u30da\u30d7\u30c1\u30c9\u6750\u6599\u306b\u3064\u3044\u3066\u554f\u3044\u5408\u308f\u305b\u308b\u969b\u306b\u3053\u308c\u3089\u306e\u8cea\u554f\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044<\/h2>\n<p>\u691c\u8a0e\u3057\u3066\u3044\u308b\u5834\u5408 <a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ghk-cu\/\">GHK-Cu<\/a> \u307e\u305f\u306f <a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ll-37\/\">LL-37<\/a>\u3001\u307e\u305a\u6b63\u78ba\u306a\u88fd\u54c1\u3068\u4ed5\u69d8\u3092\u7279\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u6b21\u306b\u3001\u5bfe\u5fdc\u3059\u308b\u30ed\u30c3\u30c8\u3067\u5229\u7528\u53ef\u80fd\u306a\u8a18\u9332\u3092\u5c0b\u306d\u3066\u304f\u3060\u3055\u3044\u3002\u30ab\u30bf\u30ed\u30b0\u306e\u8aac\u660e\u306f\u30d0\u30c3\u30c1\u30ec\u30dd\u30fc\u30c8\u306e\u4ee3\u308f\u308a\u306b\u306f\u306a\u308a\u307e\u305b\u3093\u3002<\/p>\n<p>\u3059\u3079\u3066\u306e\u5c02\u9580\u7528\u8a9e\u3092\u77e5\u3089\u306a\u304f\u3066\u3082\u3001\u3053\u306e\u30ea\u30af\u30a8\u30b9\u30c8\u3092\u4f7f\u7528\u3067\u304d\u307e\u3059\uff1a<\/p>\n<div class=\"note\">\u8a72\u5f53\u3059\u308b\u88fd\u54c1\u30ed\u30c3\u30c8\u3067\u5229\u7528\u53ef\u80fd\u306a\u8a66\u9a13\u8a18\u9332\u3092\u8aac\u660e\u3057\u3066\u304f\u3060\u3055\u3044\u3002HPLC\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\u304c\u5229\u7528\u53ef\u80fd\u306a\u5834\u5408\u306f\u3001\u5b8c\u5168\u306a\u30c8\u30ec\u30fc\u30b9\u3001\u7a4d\u5206\u8868\u3001\u8a66\u9a13\u6761\u4ef6\u3001\u304a\u3088\u3073\u30bf\u30fc\u30b2\u30c3\u30c8\u30d4\u30fc\u30af\u3092\u5272\u308a\u5f53\u3066\u308b\u6839\u62e0\u3092\u542b\u3081\u3066\u304f\u3060\u3055\u3044\u3002\u5229\u7528\u3067\u304d\u306a\u3044\u8a66\u9a13\u3092\u660e\u78ba\u306b\u7279\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/div>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e5%bc%95%e7%94%a8\/\">\u88fd\u54c1\u3068\u6587\u66f8\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u306b\u5c0b\u306d\u308b<\/a>\u3001\u307e\u305f\u306f\u8aad\u3080 <a href=\"https:\/\/nhdbio.com\/ja\/%e5%88%86%e6%9e%90%e6%89%8b%e6%b3%95\/%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e5%90%ab%e6%9c%89%e9%87%8f%e3%81%a8%e7%b4%94%e5%ba%a6%e3%81%ae%e9%96%a2%e4%bf%82\/\">\u30da\u30d7\u30c1\u30c9\u542b\u91cf\u3068\u7d14\u5ea6\u306e\u9055\u3044<\/a>.<\/p>\n<footer><strong>\u51fa\u5178\u3068\u56f3\u306e\u6ce8\u8a18<\/strong><\/p>\n<p>Technical sources checked 10 October 2026: Waters\u2019 chromatography primer, Agilent\u2019s integration guidance and Bachem\u2019s peptide quality-control guide, linked beside the relevant explanations. All three figures were drawn from teaching models. Retention times, areas and calculations are hypothetical and do not represent instrument performance, product specifications or batch results.<\/p>\n<p>AI\u652f\u63f4\u306b\u3088\u308b\u57f7\u7b46\u3068\u51fa\u5178\u78ba\u8a8d\u3002\u3053\u306e\u5165\u9580\u30ac\u30a4\u30c9\u306f\u3001\u7279\u5b9a\u306e\u7d50\u679c\u306b\u5bfe\u3059\u308b laboratory assessment \u306b\u4ee3\u308f\u308b\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002NHD\u306e\u30ab\u30bf\u30ed\u30b0\u8cc7\u6599\u306f\u7814\u7a76\u3001\u958b\u767a\u3001\u88fd\u9020\u7528\u3067\u3042\u308a\u3001\u4eba\u307e\u305f\u306f\u52d5\u7269\u3078\u306e\u4f7f\u7528\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n<\/footer>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>A step-by-step HPLC lesson with three simulated figures, a worked area-purity calculation and a checklist for reading peptide reports.<\/p>","protected":false},"author":0,"featured_media":0,"parent":865,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-871","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>How to Read a Peptide Chromatogram: Peak Area &amp; Purity | NHD<\/title>\n<meta name=\"description\" content=\"Learn to read peptide HPLC reports with three teaching figures. Check peak area, calculate purity, recognize shoulders and distinguish purity from peptide content.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ja\/\u5206\u6790\u624b\u6cd5\/\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\u306e\u89e3\u91c8\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How to Read a Peptide Chromatogram: Understanding 95% Purity\" \/>\n<meta property=\"og:description\" content=\"Learn to read peptide HPLC reports with three teaching figures. 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