{"id":3026,"date":"2026-10-03T16:08:47","date_gmt":"2026-10-03T16:08:47","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3026"},"modified":"2026-10-15T03:53:29","modified_gmt":"2026-10-15T03:53:29","slug":"%e3%83%9a%e3%83%97%e3%83%81%e3%83%89coa%e3%81%af%e6%a4%9c%e5%87%ba%e3%81%95%e3%82%8c%e3%81%9a%e3%80%81%e6%a4%9c%e5%87%ba%e5%ae%9a%e9%87%8f%e9%99%90%e7%95%8c","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ja\/peptide-coa-not-detected-detection-quantitation-limits\/","title":{"rendered":"\u201c\u300c\u4e0d\u691c\u51fa\u300d\u306f\u30bc\u30ed\u3067\u306f\u306a\u3044\uff1a\u691c\u51fa\u9650\u754c\u3068\u5b9a\u91cf\u9650\u754c\u3092\u8aad\u307f\u89e3\u304f"},"content":{"rendered":"<p>A report says \u201cnot detected.\u201d Can you write that the sample contains none of that substance? Not quite. Every method has a limit to what it can see and measure. The report needs to tell you that limit.<\/p>\n<h2>\u4f55\u304b\u3092\u898b\u308b\u3053\u3068\u3068\u3001\u305d\u308c\u3092\u6b63\u78ba\u306b\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306f\u5225\u3067\u3059<\/h2>\n<p>\u691c\u51fa\u9650\u754c\u306f\u3001\u4f4e\u30ec\u30d9\u30eb\u306e\u7269\u8cea\u3092\u305d\u306e\u65b9\u6cd5\u3067\u540c\u5b9a\u3067\u304d\u308b\u304b\u3069\u3046\u304b\u306b\u95a2\u4fc2\u3057\u307e\u3059\u3002\u5b9a\u91cf\u9650\u754c\u306f\u3001\u305d\u306e\u91cf\u3092\u9069\u5207\u306a\u6027\u80fd\u3067\u6e2c\u5b9a\u3067\u304d\u308b\u304b\u3069\u3046\u304b\u306b\u95a2\u4fc2\u3057\u307e\u3059\u3002\u304b\u3059\u304b\u306a\u30b7\u30b0\u30ca\u30eb\u306f\u3001\u305d\u306e\u91cf\u304c\u4fe1\u983c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308b\u524d\u306b\u8a8d\u8b58\u3067\u304d\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u5831\u544a\u9650\u754c\u306f\u3001\u624b\u9806\u306b\u3088\u3063\u3066\u5225\u306b\u5b9a\u7fa9\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u30ec\u30dd\u30fc\u30c8\u304c\u5b9f\u969b\u306b\u3069\u306e\u9650\u754c\u3092\u4f7f\u3063\u3066\u3044\u308b\u304b\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t17\/en\/1.png\" alt=\"Figure 1. Detection and quantitation are different questions.\"><figcaption>\u56f3 1\u3002\u691c\u51fa\u3068\u4fe1\u983c\u3067\u304d\u308b\u91cf\u306e\u6e2c\u5b9a\u306f\u3001\u7570\u306a\u308b\u554f\u3044\u306b\u7b54\u3048\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>\u4f4e\u3044\u65b9\u304b\u3089\u9ad8\u3044\u65b9\u3078\u3001\u4e00\u3064\u306e\u4f8b\u3092\u305f\u3069\u308a\u307e\u3057\u3087\u3046<\/h2>\n<p>In this teaching example, the detection limit is about 0.2 \u00b5g\/g and the quantitation limit is 0.6 \u00b5g\/g. Below the detection capability, presence cannot be reliably established by these rules.<\/p>\n<p>\u691c\u51fa\u3088\u308a\u4e0a\u3060\u304c 0.6 \u3088\u308a\u4e0b\u3067\u306f\u3001\u4fe1\u983c\u3067\u304d\u308b\u6570\u5024\u3068\u3057\u3066\u306e\u91cf\u306f\u306a\u3044\u3082\u306e\u306e\u3001\u5b58\u5728\u306e\u8a3c\u62e0\u304c\u3042\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u78ba\u7acb\u3055\u308c\u305f\u5b9a\u91cf\u7bc4\u56f2\u3067\u306f\u3001\u305d\u306e\u65b9\u6cd5\u306f\u91cf\u306e\u7d50\u679c\u3092\u652f\u6301\u3067\u304d\u307e\u3059\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t17\/en\/2.png\" alt=\"Figure 2. Illustrative thresholds: below detection, detected below quantitation, and within a supported quantitative range.\"><figcaption>Figure 2. Example detection and quantitation limits of 0.2 and 0.6 \u00b5g\/g.<\/figcaption><\/figure>\n<h2>\u305d\u306e\u9650\u754c\u5024\u306f\u3042\u306a\u305f\u306e\u8981\u4ef6\u3092\u6e80\u305f\u3057\u3066\u3044\u307e\u3059\u304b\uff1f<\/h2>\n<p>Suppose you require less than 0.1 \u00b5g\/g, but the result only supports less than 0.2 \u00b5g\/g. It does not settle whether the sample meets the stricter requirement.<\/p>\n<p>\u3069\u3061\u3089\u306e\u6570\u5024\u3082\u5c0f\u3055\u304f\u898b\u3048\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305d\u308c\u3067\u3082\u4e21\u8005\u306e\u5dee\u306f\u91cd\u8981\u3067\u3059\u3002\u540c\u3058\u7269\u8cea\u3001\u5358\u4f4d\u3001\u8a66\u6599\u57fa\u6e96\u3067\u6bd4\u8f03\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>A blank box is not \u201cnot detected\u201d<\/h2>\n<p>A blank field may mean no result was supplied. \u201cNot tested\u201d means the test was not performed. \u201cNot detected\u201d describes an outcome under a method\u2019s reporting rules. None automatically means an exact zero.<\/p>\n<p>\u8868\u793a\u3055\u308c\u305f0.000\u3082\u3001\u4e38\u3081\u3084\u51e6\u7406\u304c\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u6b63\u78ba\u306a\u5024\u3068\u3057\u3066\u6271\u3046\u524d\u306b\u3001\u3069\u306e\u3088\u3046\u306b\u7b97\u51fa\u3055\u308c\u305f\u304b\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t17\/en\/3.png\" alt=\"Figure 3. Report ND with the method, unit and reporting threshold.\"><figcaption>Figure 3. Read \u201cnot detected\u201d together with the method, unit and reporting limit.<\/figcaption><\/figure>\n<h2>\u5b8c\u5168\u306a\u8cea\u554f\u30921\u3064\u3057\u3066\u304f\u3060\u3055\u3044<\/h2>\n<p>\u3069\u306e\u7269\u8cea\u3092\u3001\u3069\u306e\u65b9\u6cd5\u3067\u3001\u3069\u306e\u5358\u4f4d\u3067\u3001\u3069\u306e\u5831\u544a\u9650\u754c\u3067\u8a66\u9a13\u3057\u305f\u304b\u3092\u5c0b\u306d\u3066\u304f\u3060\u3055\u3044\u3002\u4f8b\u3048\u3070\u3001\u30a8\u30f3\u30c9\u30c8\u30ad\u30b7\u30f3\u8a66\u9a13\u3068\u6b8b\u7559\u6eb6\u5a92\u8a66\u9a13\u306f\u7570\u306a\u308b\u7269\u8cea\u3092\u8abf\u67fb\u3057\u307e\u3059\u3002<\/p>\n<div class=\"exercise\"><strong>A report states less than 1 \u00b5g\/g. Does that establish less than 0.5 \u00b5g\/g?<\/strong><\/p>\n<details>\n<summary>\u7b54\u3048\u3092\u898b\u308b<\/summary>\n<p>No. The range from 0.5 to 1 \u00b5g\/g has not been excluded.<\/p>\n<\/details>\n<\/div>\n<h2>LL-37\u307e\u305f\u306fGHK-Cu\u306e\u7d50\u679c\u3092\u4f9d\u983c\u3059\u308b<\/h2>\n<p>LL-37\u307e\u305f\u306fGHK-Cu\u306b\u3064\u3044\u3066\u306f\u3001\u6e2c\u5b9a\u3057\u305f\u3044\u7269\u8cea\u3068\u5fc5\u8981\u306a\u5831\u544a\u80fd\u529b\u3092\u6307\u5b9a\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5b9f\u969b\u306b\u3069\u306e\u8a66\u9a13\u304c\u884c\u308f\u308c\u305f\u304b\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002HPLC\u7d14\u5ea6\u306f\u30a8\u30f3\u30c9\u30c8\u30ad\u30b7\u30f3\u7d50\u679c\u3092\u63d0\u4f9b\u3057\u307e\u305b\u3093\u3002<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ll-37\/\">LL-37<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ghk-cu\/\">GHK-Cu<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e5%bc%95%e7%94%a8\/\">\u8cc7\u6750\u304a\u3088\u3073\u6587\u66f8\u306e\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u3068\u76f8\u8ac7\u3059\u308b<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ja\/%e5%88%86%e6%9e%90%e6%89%8b%e6%b3%95\/%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e3%81%aecoa%e3%81%ae%e8%aa%ad%e3%81%bf%e6%96%b9\/\">\u5206\u6790\u30ac\u30a4\u30c9\u3092\u7d9a\u3051\u308b<\/a><\/p>\n<footer><strong>\u51fa\u5178\u3068\u4f8b\u306e\u6ce8\u8a18<\/strong><\/p>\n<p>\u6570\u5024\u3068\u66f2\u7dda\u306f\u6559\u80b2\u7528\u306e\u4f8b\u3067\u3042\u308a\u3001\u88fd\u54c1\u30d0\u30c3\u30c1\u306e\u8a66\u9a13\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u51fa\u5178\u78ba\u8a8d\u65e5\uff1a11 \u5e74 2026 \u6708\u3002<\/p>\n<ul>\n<li><a href=\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\">ICH Q2(R2) \u00b7 analytical validation<\/a><\/li>\n<\/ul>\n<p>AI\u652f\u63f4\u306b\u3088\u308b\u57f7\u7b46\u304a\u3088\u3073\u60c5\u5831\u6e90\u306e\u30ec\u30d3\u30e5\u30fc\u3002\u30ab\u30bf\u30ed\u30b0\u8cc7\u6599\u306f\u7814\u7a76\u3001\u958b\u767a\u3001\u88fd\u9020\u7528\u3067\u3042\u308a\u3001\u4eba\u307e\u305f\u306f\u52d5\u7269\u3078\u306e\u4f7f\u7528\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u300c\u691c\u51fa\u3055\u308c\u306a\u304b\u3063\u305f\u300d\u3053\u3068\u304c\u4f55\u3092\u6559\u3048\u3066\u304f\u308c\u308b\u306e\u304b\u3001\u305d\u3057\u3066\u3042\u3068\u3069\u306e\u6570\u5024\u304c\u5fc5\u8981\u306a\u306e\u304b\u3092\u5b66\u3073\u307e\u3057\u3087\u3046\u3002<\/p>","protected":false},"author":1,"featured_media":3054,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"318,344","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and clearly labeled examples","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Reference data and teaching examples are separated from NHD lot results; product specifications and availability require confirmation.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"AI-assisted drafting, source checks, translation and generated comic illustration. This is not independent peer review or supplied-lot verification.","pim_sources":"[{\"title\":\"ICH Q2(R2): Validation of Analytical Procedures\",\"note\":\"International Council for Harmonisation. Step 4, adopted November 2023; error-corrected 2025.\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2%28R2%29_Guideline_2023_1130_ErrorCorrection_2025.pdf\"},{\"title\":\"Teaching reference \u2014 database.ich.org \u2014 ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\",\"note\":\"\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\"},{\"title\":\"ICH Q2(R2) Validation of Analytical Procedures\",\"note\":\"ICH Q2(R2) Validation of Analytical Procedures\",\"url\":\"https:\/\/www.ema.europa.eu\/en\/scientific-guidelines\/ich-q2r2-validation-analytical-procedures\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3026","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>\u201cNot Detected\u201d Is Not Zero: Read Detection and Quantitation Limits | NHD<\/title>\n<meta name=\"description\" content=\"Learn what \u201cnot detected\u201d tells you, and what number you still need.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ja\/\u30da\u30d7\u30c1\u30c9coa\u306f\u691c\u51fa\u3055\u308c\u305a\u3001\u691c\u51fa\u5b9a\u91cf\u9650\u754c\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u201cNot Detected\u201d Is Not Zero: Read Detection and Quantitation Limits\" \/>\n<meta property=\"og:description\" content=\"Learn what \u201cnot detected\u201d tells you, and what number you still need.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nhdbio.com\/ja\/\u30da\u30d7\u30c1\u30c9coa\u306f\u691c\u51fa\u3055\u308c\u305a\u3001\u691c\u51fa\u5b9a\u91cf\u9650\u754c\/\" \/>\n<meta property=\"og:site_name\" content=\"NHD\" \/>\n<meta property=\"article:published_time\" content=\"2026-10-03T16:08:47+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-10-15T03:53:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/limits-cover-1024x683.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1024\" \/>\n\t<meta property=\"og:image:height\" content=\"683\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"NHD Technical Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"NHD Technical Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"2\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/\"},\"author\":{\"name\":\"NHD Technical Team\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/person\\\/0ffb0dec7fda0f720073fc468fac6fc1\"},\"headline\":\"\u201cNot Detected\u201d Is Not Zero: Read Detection and Quantitation Limits\",\"datePublished\":\"2026-10-03T16:08:47+00:00\",\"dateModified\":\"2026-10-15T03:53:29+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/\"},\"wordCount\":469,\"publisher\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-coa-not-detected-detection-quantitation-limits\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/limits-cover.png\",\"articleSection\":[\"Evidence &amp; 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