{"id":3027,"date":"2026-10-03T16:09:26","date_gmt":"2026-10-03T16:09:26","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3027"},"modified":"2026-10-11T07:38:55","modified_gmt":"2026-10-11T07:38:55","slug":"%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e3%82%b9%e3%83%88%e3%83%83%e3%82%af%e5%b8%8c%e9%87%88%e3%81%ae%e6%9c%80%e7%b5%82dmso%e3%83%91%e3%83%bc%e3%82%bb%e3%83%b3%e3%83%86%e3%83%bc%e3%82%b8","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ja\/peptide-stock-dilution-final-dmso-percentage\/","title":{"rendered":"\u30da\u30d7\u30c1\u30c9\u30b9\u30c8\u30c3\u30af\u3092\u5e0c\u91c8\u3057\u307e\u3059\u304b\uff1f\u6700\u7d42\u7684\u306aDMSO\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3092\u6570\u3048\u3066\u304f\u3060\u3055\u3044"},"content":{"rendered":"<p>\u6fc3\u7e2e\u30da\u30d7\u30c1\u30c9\u30b9\u30c8\u30c3\u30af\u3092\u5c11\u91cf\u52a0\u3048\u308b\u3068\u3001\u305d\u306e\u6eb6\u5a92\u3082\u4e00\u7dd2\u306b\u52a0\u308f\u308a\u307e\u3059\u3002\u30b9\u30c8\u30c3\u30af\u306bDMSO\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u30da\u30d7\u30c1\u30c9\u3060\u3051\u3067\u306a\u304f\u305d\u306e\u6db2\u4f53\u3082\u8ffd\u8de1\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e2\u3064\u306e\u8a08\u7b97\u306f\u7570\u306a\u308b\u554f\u3044\u306b\u7b54\u3048\u308b\u3082\u306e\u3067\u3059\u3002<\/p>\n<h2>\u307e\u305a\u6eb6\u5a92\u306e\u4f53\u7a4d\u3092\u6570\u3048\u308b<\/h2>\n<p>DMSO\u306f\u3001\u7814\u7a76\u7528\u30b9\u30c8\u30c3\u30af\u6eb6\u6db2\u3067\u6642\u3005\u4f7f\u7528\u3055\u308c\u308b\u6eb6\u5a92\u3067\u3059\u3002\u6700\u7d42\u7684\u306a\u4f53\u7a4d\u30d1\u30fc\u30bb\u30f3\u30c8\u3092\u8a08\u7b97\u3059\u308b\u306b\u306f\u3001DMSO\u304c\u4f55\u30de\u30a4\u30af\u30ed\u30ea\u30c3\u30c8\u30eb\u52a0\u3048\u3089\u308c\u305f\u304b\u3092\u6c42\u3081\u3001\u6700\u7d42\u6df7\u5408\u6db2\u306e\u4f53\u7a4d\u3067\u5272\u308a\u307e\u3059\u3002<\/p>\n<p>\u7d14\u7c8b\u306aDMSO\u3067\u4f5c\u3063\u305f\u30b9\u30c8\u30c3\u30af\u6eb6\u6db2\u3067\u306f\u3001\u79fb\u3057\u305f\u4e00\u30de\u30a4\u30af\u30ed\u30ea\u30c3\u30c8\u30eb\u306b\u3064\u304d\u4e00\u30de\u30a4\u30af\u30ed\u30ea\u30c3\u30c8\u30eb\u306eDMSO\u304c\u52a0\u308f\u308a\u307e\u3059\u300220% DMSO\u3092\u542b\u3080\u30b9\u30c8\u30c3\u30af\u6eb6\u6db2\u3067\u306f\u3001\u52a0\u308f\u308b\u91cf\u306f\u305d\u308c\u3088\u308a\u5c11\u306a\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t05\/en\/1.png\" alt=\"Figure 1. A transfer carries both peptide and solvent into the final mixture.\"><figcaption>\u56f31\u3002\u30da\u30d7\u30c1\u30c9\u3068\u6eb6\u5a92\u306e\u4e21\u65b9\u304c\u30b9\u30c8\u30c3\u30af\u306e\u5206\u53d6\u91cf\u3068\u3068\u3082\u306b\u79fb\u52d5\u3057\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>\u7d14\u7c8b\u306aDMSO\u3092\u7528\u3044\u305f\u4f8b<\/h2>\n<p>Assume a 1 mM peptide stock in pure DMSO. Transfer 10 \u00b5L and make the final mixture up to 1,000 \u00b5L. The peptide concentration is 1,000 \u00b5M \u00d7 10 \u00f7 1,000 = 10 \u00b5M.<\/p>\n<p>The DMSO percentage is 10 \u00f7 1,000 \u00d7 100% = 1% by volume. These numbers describe the same mixture, but one describes peptide concentration and the other solvent proportion.<\/p>\n<p class=\"formula\">10 \u00b5L DMSO \u00f7 1,000 \u00b5L final volume \u00d7 100% = 1%<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t05\/en\/2.png\" alt=\"Figure 2. 10 \u00b5L of a stock in 100% DMSO made up to 1,000 \u00b5L gives 1% DMSO by volume.\"><figcaption>Figure 2. In this example, 10 \u00b5L of pure DMSO in a final 1,000 \u00b5L gives 1% DMSO.<\/figcaption><\/figure>\n<h2>\u30b9\u30c8\u30c3\u30af\u304c20% DMSO\u3092\u542b\u3080\u5834\u5408\u3001\u4f55\u304c\u5909\u308f\u308a\u307e\u3059\u304b\uff1f<\/h2>\n<p>The same 10 \u00b5L aliquot now carries 10 \u00d7 0.20 = 2 \u00b5L of DMSO. In a final 1,000 \u00b5L mixture, that is 0.2% by volume.<\/p>\n<p>\u8907\u6570\u306e\u8a66\u85ac\u304cDMSO\u3092\u542b\u3080\u5834\u5408\u306f\u3001\u6700\u7d42\u5bb9\u91cf\u3067\u5272\u308b\u524d\u306b\u3001\u305d\u308c\u3089\u3059\u3079\u3066\u306eDMSO\u5bc4\u4e0e\u3092\u5408\u8a08\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30da\u30d7\u30c1\u30c9\u30b9\u30c8\u30c3\u30af\u3060\u3051\u3092\u6570\u3048\u3066\u306f\u3044\u3051\u307e\u305b\u3093\u3002<\/p>\n<h2>\u6eb6\u5a92\u5bfe\u7167\u304c\u91cd\u8981\u306a\u7406\u7531<\/h2>\n<p>\u30a6\u30a7\u30eb\u304c\u7570\u306a\u308b\u30b9\u30c8\u30c3\u30af\u4f53\u7a4d\u3092\u53d7\u3051\u53d6\u308b\u5834\u5408\u3001\u305d\u308c\u3089\u306eDMSO\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3082\u7570\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u9069\u5207\u306a\u6eb6\u5a92\u5bfe\u7167\u306f\u3001\u3059\u3079\u3066\u306e\u5909\u5316\u3092\u30da\u30d7\u30c1\u30c9\u306b\u5e30\u5c5e\u3055\u305b\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u6eb6\u5a92\u306e\u5bc4\u4e0e\u3092\u8abf\u3079\u308b\u306e\u306b\u5f79\u7acb\u3061\u307e\u3059\u3002<\/p>\n<p>\u7279\u5b9a\u306e\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u304c\u8a31\u5bb9\u3067\u304d\u308b\u304b\u3069\u3046\u304b\u306f\u3001\u7814\u7a76\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002\u3053\u3053\u3067\u306e1%\u30680.2%\u306f\u8a08\u7b97\u5024\u3067\u3042\u308a\u3001\u63a8\u5968\u9650\u754c\u5024\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t05\/en\/3.png\" alt=\"Figure 3. Track solvent fraction alongside the peptide concentration at every level.\"><figcaption>\u56f33\u3002\u30da\u30d7\u30c1\u30c9\u6fc3\u5ea6\u3068\u4f75\u305b\u3066\u6eb6\u5a92\u66dd\u9732\u91cf\u3092\u8a08\u7b97\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/figcaption><\/figure>\n<h2>\u6df7\u5408\u6eb6\u5a92\u30b9\u30c8\u30c3\u30af\u306e\u30a2\u30ea\u30b3\u30fc\u30c8\u3092\u8a66\u3057\u3066\u304f\u3060\u3055\u3044<\/h2>\n<div class=\"exercise\"><strong>Transfer 5 \u00b5L of a stock containing 40% DMSO into a final 1,000 \u00b5L. What is the final DMSO percentage?<\/strong><\/p>\n<details>\n<summary>\u7b54\u3048\u3092\u898b\u308b<\/summary>\n<p>The aliquot contains 5 \u00d7 0.40 = 2 \u00b5L of DMSO. The final percentage is 2 \u00f7 1,000 \u00d7 100% = 0.2% by volume.<\/p>\n<\/details>\n<\/div>\n<p>\u8a08\u7b97\u306e\u6a2a\u306b\u30b9\u30c8\u30c3\u30af\u306e\u6eb6\u5a92\u7d44\u6210\u3068\u6700\u7d42\u4f53\u7a4d\u3092\u66f8\u3044\u3066\u304f\u3060\u3055\u3044\u3002\u30b9\u30c8\u30c3\u30af\u306e\u4f53\u7a4d\u3060\u3051\u3067\u306f\u518d\u73fe\u3059\u308b\u306e\u306b\u5341\u5206\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<h2>LL-37\u307e\u305f\u306fSS-31\u306e\u4f5c\u696d\u3092\u8a08\u753b\u3059\u308b\u969b\u306f\u6eb6\u5a92\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044<\/h2>\n<p>LL-37\u307e\u305f\u306fSS-31\u306b\u3064\u3044\u3066\u306f\u3001\u4f9b\u7d66\u3055\u308c\u305f\u6750\u6599\u306b\u3069\u306e\u6eb6\u5a92\u304c\u9069\u3057\u3066\u3044\u308b\u304b\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u6b21\u306b\u3001\u305d\u306e\u6eb6\u5a92\u304c\u8a08\u753b\u3057\u3066\u3044\u308b\u6700\u7d42\u6df7\u5408\u6db2\u306b\u3069\u306e\u7a0b\u5ea6\u5bc4\u4e0e\u3059\u308b\u304b\u3092\u8a08\u7b97\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u88fd\u54c1\u540d\u306f\u666e\u904d\u7684\u306aDMSO\u8abf\u88fd\u6cd5\u3092\u78ba\u7acb\u3059\u308b\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ll-37\/\">LL-37<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ss-31-%e3%82%a8%e3%83%a9%e3%83%9f%e3%83%97%e3%83%ac%e3%83%81%e3%83%89\/\">SS-31<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e5%bc%95%e7%94%a8\/\">\u8cc7\u6750\u304a\u3088\u3073\u6587\u66f8\u306e\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u3068\u76f8\u8ac7\u3059\u308b<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ja\/%e5%88%86%e6%9e%90%e6%89%8b%e6%b3%95\/%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e5%90%ab%e6%9c%89%e9%87%8f%e3%81%a8%e7%b4%94%e5%ba%a6%e3%81%ae%e9%96%a2%e4%bf%82\/\">\u5206\u6790\u30ac\u30a4\u30c9\u3092\u7d9a\u3051\u308b<\/a><\/p>\n<footer><strong>\u51fa\u5178\u3068\u4f8b\u306e\u6ce8\u8a18<\/strong><\/p>\n<p>\u6570\u5024\u3068\u66f2\u7dda\u306f\u6559\u80b2\u7528\u306e\u4f8b\u3067\u3042\u308a\u3001\u88fd\u54c1\u30d0\u30c3\u30c1\u306e\u8a66\u9a13\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u51fa\u5178\u78ba\u8a8d\u65e5\uff1a11 \u5e74 2026 \u6708\u3002<\/p>\n<ul>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/support\/calculators-and-apps\/solution-dilution-calculator\">Sigma-Aldrich \u00b7 solution dilution calculator<\/a><\/li>\n<li><a href=\"https:\/\/www.sigmaaldrich.com\/content\/dam\/sigma-aldrich\/docs\/Sigma\/General_Information\/peptide_handling_guide.pdf\">Sigma-Aldrich \u00b7 peptide handling guide<\/a><\/li>\n<\/ul>\n<p>AI\u652f\u63f4\u306b\u3088\u308b\u57f7\u7b46\u304a\u3088\u3073\u60c5\u5831\u6e90\u306e\u30ec\u30d3\u30e5\u30fc\u3002\u30ab\u30bf\u30ed\u30b0\u8cc7\u6599\u306f\u7814\u7a76\u3001\u958b\u767a\u3001\u88fd\u9020\u7528\u3067\u3042\u308a\u3001\u4eba\u307e\u305f\u306f\u52d5\u7269\u3078\u306e\u4f7f\u7528\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u30da\u30d7\u30c1\u30c9\u30b9\u30c8\u30c3\u30af\u3092\u5e0c\u91c8\u3059\u308b\u969b\u306b\u3001\u6700\u7d42\u6df7\u5408\u7269\u306b\u3069\u308c\u3060\u3051\u306eDMSO\u304c\u5165\u308b\u304b\u3092\u8ffd\u8de1\u3057\u307e\u3059\u3002<\/p>","protected":false},"author":1,"featured_media":3057,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and clearly labeled examples","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Reference data and teaching examples are separated from NHD lot results; product specifications and availability require confirmation.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"AI-assisted drafting, source checks, translation and generated comic illustration. This is not independent peer review or supplied-lot verification.","pim_sources":"[{\"title\":\"Thermo Fisher: handling and storage instructions for standard peptides\",\"note\":\"Thermo Fisher: handling and storage instructions for standard peptides. Accessed 2026-10-03.\",\"url\":\"https:\/\/documents.thermofisher.com\/TFS-Assets\/BID\/Reference-Materials\/handling-storage-instruction-standard-peptides.pdf\"},{\"title\":\"Teaching reference \u2014 www.sigmaaldrich.com \u2014 solution-dilution-calculator\",\"note\":\"\",\"url\":\"https:\/\/www.sigmaaldrich.com\/US\/en\/support\/calculators-and-apps\/solution-dilution-calculator\"},{\"title\":\"Teaching reference \u2014 www.sigmaaldrich.com \u2014 peptide_handling_guide.pdf\",\"note\":\"\",\"url\":\"https:\/\/www.sigmaaldrich.com\/content\/dam\/sigma-aldrich\/docs\/Sigma\/General_Information\/peptide_handling_guide.pdf\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3027","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Diluting a Peptide Stock? Count the Final DMSO Percentage | NHD<\/title>\n<meta name=\"description\" content=\"Track how much DMSO enters the final mixture when you dilute a peptide stock.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ja\/\u30da\u30d7\u30c1\u30c9\u30b9\u30c8\u30c3\u30af\u5e0c\u91c8\u306e\u6700\u7d42dmso\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Diluting a Peptide Stock? 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