{"id":3081,"date":"2026-10-03T20:25:51","date_gmt":"2026-10-03T20:25:51","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3081"},"modified":"2026-10-03T20:25:51","modified_gmt":"2026-10-03T20:25:51","slug":"%e3%83%9a%e3%83%97%e3%83%81%e3%83%89lc-ms%e3%81%ae%e9%9b%bb%e8%8d%b7%e7%8a%b6%e6%85%8bm-z%e3%81%ae%e7%b0%a1%e5%8d%98%e3%81%aa%e4%be%8b","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ja\/peptide-lcms-charge-state-mz-simple-example\/","title":{"rendered":"\u306a\u305c\u30da\u30d7\u30c1\u30c9\u8cea\u91cf\u30b9\u30da\u30af\u30c8\u30eb\u306f\u4e88\u60f3\u8cea\u91cf\u306e\u534a\u5206\u3092\u793a\u3059\u306e\u3067\u3059\u304b\uff1f"},"content":{"rendered":"<p>\u3042\u306a\u305f\u306e\u30da\u30d7\u30c1\u30c9\u6587\u66f8\u306b\u306f 2,000 Da \u4ed8\u8fd1\u306e\u5206\u5b50\u91cf\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30b9\u30da\u30af\u30c8\u30eb\u306b\u306f 1,001 \u4ed8\u8fd1\u306b\u30d4\u30fc\u30af\u304c\u3042\u308a\u307e\u3059\u3002\u4f9b\u7d66\u5143\u306f\u9593\u9055\u3063\u305f\u7269\u8cea\u3092\u9001\u3063\u305f\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u305d\u306e\u6570\u5024\u3060\u3051\u3067\u306f\u7b54\u3048\u3089\u308c\u307e\u305b\u3093\u3002\u8cea\u91cf\u5206\u6790\u8a08\u306f\u8cea\u91cf\u3092\u96fb\u8377\u3067\u5272\u3063\u305f\u5024\u3092\u6e2c\u5b9a\u3057\u3001\u901a\u5e38 m\/z \u3068\u8868\u8a18\u3057\u307e\u3059\u3002<\/p>\n<h2>1\u3064\u306e\u5206\u5b50\u304c\u8907\u6570\u306e\u96fb\u8377\u3092\u5e2f\u3073\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059<\/h2>\n<p>Think of the instrument\u2019s number as a divided value. In positive electrospray, a peptide can pick up more than one proton. Two charges divide the ion\u2019s mass by two; three divide it by three. Thermo Fisher\u2019s introduction explains why mass spectrometry reports mass-to-charge values rather than a simple weight.<\/p>\n<h2>\u8a08\u7b97\u4f8b\uff1a2,000 Da \u306f\u7d04 1,001 m\/z \u306b\u306a\u308a\u307e\u3059<\/h2>\n<p>Take an imaginary peptide with a neutral mass of 2,000 Da. For proton-only positive ions, use m\/z = (M + z \u00d7 1.0073) \u00f7 z, where M is neutral mass and z is charge. The approximate proton mass is 1.0073 Da. At charge +1, the result is 2,001.0073; at +2, it is 1,001.0073; at +3, it is 667.6739.<\/p>\n<p>\u3053\u308c\u3089\u306f\u8a08\u7b97\u306b\u3088\u308b\u6559\u80b2\u7528\u306e\u5024\u3067\u3042\u308a\u3001NHD \u306e\u8a66\u9a13\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u540c\u3058\u4eee\u60f3\u5206\u5b50\u306e3\u3064\u306e\u96fb\u8377\u72b6\u614b\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002\u5c0f\u3055\u3044\u6570\u5024\u306f\u30da\u30d7\u30c1\u30c9\u9396\u304c\u534a\u5206\u306b\u5207\u308c\u305f\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u305b\u3093\u3002<\/p>\n<h2>\u5831\u544a\u66f8\u304c\u3069\u306e\u6570\u5024\u3092\u793a\u3057\u3066\u3044\u308b\u304b\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044<\/h2>\n<p>\u96fb\u8377\u306e\u5272\u308a\u5f53\u3066\u3092\u78ba\u8a8d\u3057\u3001\u5831\u544a\u304c\u751f\u306em\/z\u3092\u793a\u3057\u3066\u3044\u308b\u306e\u304b\u3001\u518d\u69cb\u6210\u3055\u308c\u305f\u4e2d\u6027\u8cea\u91cf\u3092\u793a\u3057\u3066\u3044\u308b\u306e\u304b\u3092\u78ba\u304b\u3081\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001\u5358\u540c\u4f4d\u4f53\u8cea\u91cf\u3068\u5e73\u5747\u8cea\u91cf\u306e\u3069\u3061\u3089\u3092\u4f7f\u3063\u3066\u3044\u308b\u304b\u3082\u5c0b\u306d\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u3089\u306f\u540c\u4f4d\u4f53\u3092\u8981\u7d04\u3059\u308b\u7570\u306a\u308b\u65b9\u6cd5\u3067\u3059\u3002\u30ca\u30c8\u30ea\u30a6\u30e0\u4ed8\u52a0\u4f53\u3001\u4ed6\u306e\u30a4\u30aa\u30f3\u5f62\u614b\u3001\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u7570\u306a\u308b\u89e3\u91c8\u304c\u5fc5\u8981\u3067\u3001\u5358\u7d14\u306a\u30d7\u30ed\u30c8\u30f3\u306e\u307f\u306e\u5f0f\u3067\u306f\u5bfe\u5fdc\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n<h2>\u8cea\u91cf\u306e\u4e00\u81f4\u306f\u6709\u7528\u3067\u3059\u304c\u3001\u305d\u308c\u3060\u3051\u304c\u54c1\u8cea\u30c1\u30a7\u30c3\u30af\u306e\u3059\u3079\u3066\u3067\u306f\u3042\u308a\u307e\u305b\u3093<\/h2>\n<p>\u8cea\u91cf\u306e\u4e00\u81f4\u306f\u63d0\u6848\u3055\u308c\u305f\u540c\u4e00\u6027\u3092\u652f\u6301\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u305d\u308c\u81ea\u4f53\u3067\u3001\u914d\u5217\u9806\u5e8f\u3001\u7d14\u5ea6\u3001\u7c89\u672b\u4e2d\u306e\u30da\u30d7\u30c1\u30c9\u91cf\u3092\u8a3c\u660e\u3059\u308b\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u8cea\u91cf\u306e\u7d50\u679c\u306f\u3001\u5408\u610f\u3055\u308c\u305f\u5206\u6790\u8a18\u9332\u3068\u4f75\u305b\u3066\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u79c1\u305f\u3061\u306e <a href=\"https:\/\/nhdbio.com\/ja\/%e5%88%86%e6%9e%90%e6%89%8b%e6%b3%95\/lc-ms-%e5%90%8c%e4%b8%80%e6%80%a7%e6%a4%9c%e8%a8%bc\/\">LC\u2013MS\u306b\u3088\u308b\u540c\u5b9a\u30ac\u30a4\u30c9<\/a> \u304c\u305d\u306e\u533a\u5225\u3092\u8aac\u660e\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u30ab\u30bf\u30ed\u30b0\u54c1\u76ee\u3092\u8cfc\u5165\u3057\u307e\u3059\u304b\u3001\u4f8b\u3048\u3070 <a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ll-37\/\">LL-37<\/a>\u3001\u305d\u308c\u3068\u3082\u30ab\u30b9\u30bf\u30e0\u914d\u5217\u3092\u4f9d\u983c\u3057\u307e\u3059\u304b\uff1f <a href=\"https:\/\/nhdbio.com\/ja\/%e5%bc%95%e7%94%a8\/\">NHD \u306b\u6b63\u78ba\u306a\u7269\u8cea\u3092\u9001\u308a\u3001\u3069\u306e\u540c\u4e00\u6027\u8a18\u9332\u304c\u5229\u7528\u53ef\u80fd\u304b\u3092\u5c0b\u306d\u3066\u304f\u3060\u3055\u3044<\/a>. Confirm the scope before ordering; this example does not establish any supplied lot\u2019s identity.<\/p>\n<p>\u5b9f\u9a13\u7814\u7a76\u5c02\u7528\u3002\u4eba\u307e\u305f\u306f\u52d5\u7269\u3078\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n<h2>\u53c2\u8003\u6587\u732e<\/h2>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/pl\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\">Thermo Fisher: \u8cea\u91cf\u5206\u6790\u306e\u6982\u8981<\/a><\/li>\n<li><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/posters\/po-80036-asms22-charge-assignment-of-isotopically-po80036-en.pdf\">Thermo Fisher: \u540c\u4f4d\u4f53\u5206\u96e2\u3055\u308c\u305f\u8cea\u91cf\u30c7\u30fc\u30bf\u306e\u96fb\u8377\u306e\u5272\u308a\u5f53\u3066<\/a><\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p>\u3042\u306a\u305f\u306e\u30da\u30d7\u30c1\u30c9\u6587\u66f8\u306b\u306f 2,000 Da \u4ed8\u8fd1\u306e\u5206\u5b50\u91cf\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30b9\u30da\u30af\u30c8\u30eb\u306b\u306f 1,001 \u4ed8\u8fd1\u306b\u30d4\u30fc\u30af\u304c\u3042\u308a\u307e\u3059\u3002\u4f9b\u7d66\u5143\u306f\u9593\u9055\u3063\u305f\u7269\u8cea\u3092\u9001\u3063\u305f\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u305d\u306e\u6570\u5024\u3060\u3051\u3067\u306f\u7b54\u3048\u3089\u308c\u307e\u305b\u3093\u3002\u8cea\u91cf\u5206\u6790\u8a08\u306f\u8cea\u91cf\u3092\u96fb\u8377\u3067\u5272\u3063\u305f\u5024\u3092\u6e2c\u5b9a\u3057\u3001\u901a\u5e38 m\/z \u3068\u8868\u8a18\u3057\u307e\u3059\u3002<\/p>","protected":false},"author":1,"featured_media":3083,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and labeled calculations","pim_reviewer":"Codex AI editorial","pim_reviewed_date":"2026-10-04","pim_editorial_note":"Examples are calculations, not NHD lot results. Confirm project feasibility and document availability.","pim_review_status":"reviewed","pim_review_reason":"Source, arithmetic and search-intent checks; no independent human technical review recorded.","pim_creation_method":"AI-assisted writing, source checks and illustrated cover.","pim_sources":"[{\"title\":\"Thermo Fisher: Overview of mass spectrometry\",\"url\":\"https:\/\/www.thermofisher.com\/pl\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\",\"note\":\"Technical reference; numerical planning examples are original calculations.\"},{\"title\":\"Thermo Fisher: Charge assignment of isotopically resolved mass data\",\"url\":\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/posters\/po-80036-asms22-charge-assignment-of-isotopically-po80036-en.pdf\",\"note\":\"Technical reference; numerical planning examples are original calculations.\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3081","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Why does a peptide mass spectrum show half the expected mass? - NHD<\/title>\n<meta name=\"description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ja\/\u30da\u30d7\u30c1\u30c9lc-ms\u306e\u96fb\u8377\u72b6\u614bm-z\u306e\u7c21\u5358\u306a\u4f8b\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Why does a peptide mass spectrum show half the expected mass?\" \/>\n<meta property=\"og:description\" content=\"Your peptide document lists a molecular mass near 2,000 Da, but the spectrum has a peak near 1,001. Has the supplier sent the wrong material? That number alone cannot answer the question. A mass spectrometer measures mass divided by charge, usually written as m\/z.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nhdbio.com\/ja\/\u30da\u30d7\u30c1\u30c9lc-ms\u306e\u96fb\u8377\u72b6\u614bm-z\u306e\u7c21\u5358\u306a\u4f8b\/\" \/>\n<meta property=\"og:site_name\" content=\"NHD\" \/>\n<meta property=\"article:published_time\" content=\"2026-10-03T20:25:51+00:00\" \/>\n<meta name=\"author\" content=\"NHD Technical Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"NHD Technical Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"2\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\"},\"author\":{\"name\":\"NHD Technical Team\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/person\\\/0ffb0dec7fda0f720073fc468fac6fc1\"},\"headline\":\"Why does a peptide mass spectrum show half the expected mass?\",\"datePublished\":\"2026-10-03T20:25:51+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\"},\"wordCount\":378,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/charge-cover.png\",\"articleSection\":[\"Evidence &amp; Quality Guides\"],\"inLanguage\":\"ja\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\",\"url\":\"https:\\\/\\\/nhdbio.com\\\/peptide-lcms-charge-state-mz-simple-example\\\/\",\"name\":\"Why does a peptide mass spectrum show half the expected mass? 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