{"id":3086,"date":"2026-10-03T20:25:53","date_gmt":"2026-10-03T20:25:53","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3086"},"modified":"2026-10-11T07:41:08","modified_gmt":"2026-10-11T07:41:08","slug":"%e9%87%8d%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e6%a8%99%e8%ad%98%e3%81%ae%e8%b3%aa%e9%87%8f%e3%82%b7%e3%83%95%e3%83%88%e3%81%a8%e9%9b%bb%e8%8d%b7%e3%81%ae%e4%be%8b","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ja\/heavy-peptide-label-mass-shift-charge-example\/","title":{"rendered":"8 Da \u306e\u91cd\u6a19\u8b58\u306a\u306e\u306b 4 \u306e m\/z \u30b7\u30d5\u30c8\uff1f\u96fb\u8377\u304c\u305d\u306e\u9055\u3044\u3092\u8aac\u660e\u3057\u307e\u3059"},"content":{"rendered":"<p>\u6a19\u8b58\u306f\u7d048 Da\u3092\u52a0\u3048\u307e\u3059\u304c\u3001\u30b9\u30da\u30af\u30c8\u30eb\u306f\u308f\u305a\u304b4\u3057\u304b\u30b7\u30d5\u30c8\u3057\u307e\u305b\u3093\u3002\u3053\u308c\u306f\u3001\u4e00\u81f4\u3059\u308b\u4e8c\u91cd\u96fb\u8377\u30a4\u30aa\u30f3\u306b\u307e\u3055\u306b\u671f\u5f85\u3055\u308c\u308b\u901a\u308a\u306e\u7d50\u679c\u3067\u3059\u3002\u91cd\u8981\u306a\u306e\u306f\u3001\u8cea\u91cf\u30b9\u30da\u30af\u30c8\u30eb\u306e\u8ef8\u306f\u3057\u3070\u3057\u3070\u5168\u5206\u5b50\u8cea\u91cf\u3067\u306f\u306a\u304fm\/z\u3092\u793a\u3059\u3068\u3044\u3046\u70b9\u3067\u3059\u3002<\/p>\n<h2>\u306a\u305c\u6a19\u8b58\u30da\u30d7\u30c1\u30c9\u306f\u91cd\u304f\u306a\u308b\u306e\u3067\u3059\u304b\uff1f<\/h2>\n<p>\u5b89\u5b9a\u540c\u4f4d\u4f53\u6a19\u8b58\u306f\u3001\u9078\u629e\u3055\u308c\u305f\u539f\u5b50\u3092\u6307\u5b9a\u3055\u308c\u305f\u4f4d\u7f6e\u3067\u6307\u5b9a\u3055\u308c\u305f\u91cd\u3044\u540c\u4f4d\u4f53\u306b\u7f6e\u304d\u63db\u3048\u307e\u3059\u3002\u7279\u5b9a\u3055\u308c\u3066\u3044\u306a\u3044\u91cd\u91d1\u5c5e\u3092\u7d50\u5408\u3059\u308b\u3068\u3044\u3046\u610f\u5473\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u914d\u5217\u3068\u6a19\u8b58\u4f4d\u7f6e\u3092\u660e\u8a18\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>This lesson uses a deliberately rounded +8.0000 Da example. A real label\u2019s exact mass must be calculated from its actual isotope composition.<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t15\/en\/1.png\" alt=\"\u56f31\u3002\u91cd\u3044\u6a19\u8b58\u306f\u6307\u5b9a\u3055\u308c\u305f\u914d\u5217\u3092\u4fdd\u3061\u306a\u304c\u3089\u540c\u4f4d\u4f53\u7d44\u6210\u3092\u5909\u5316\u3055\u305b\u307e\u3059\u3002\"><figcaption>\u56f31\u3002\u5b9a\u7fa9\u3055\u308c\u305f\u540c\u4f4d\u4f53\u7f6e\u63db\u306b\u3088\u308a\u3001\u6a19\u8b58\u5206\u5b50\u306f\u91cd\u304f\u306a\u308a\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>\u306a\u305c8 Da\u304c\u8ef8\u4e0a\u30674\u306b\u306a\u308b\u306e\u304b<\/h2>\n<p>\u305d\u308c\u4ee5\u5916\u306f\u4e00\u81f4\u3059\u308b\u30a4\u30aa\u30f3\u306b\u3064\u3044\u3066\u3001\u5206\u5b50\u8cea\u91cf\u306e\u5dee\u3092\u305d\u306e\u96fb\u8377\u3067\u5272\u308a\u307e\u3059\u3002\u96fb\u8377\u304c1\u3064\u306a\u3089m\/z\u30b7\u30d5\u30c8\u306f8\u30012\u3064\u306a\u30894\u30013\u3064\u306a\u3089\u7d042.6667\u3067\u3059\u3002<\/p>\n<p>\u5206\u5b50\u306f\u540c\u3058\u8cea\u91cf\u3092\u5f97\u305f\u307e\u307e\u3067\u3059\u3002\u5909\u308f\u3063\u305f\u306e\u306f\u3001\u8ef8\u304c\u305d\u306e\u5dee\u3092\u8868\u793a\u3059\u308b\u65b9\u6cd5\u3060\u3051\u3067\u3059\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t15\/en\/2.png\" alt=\"\u56f32\u3002\u4eee\u5b9a\u306e+8.0000 Da\u6a19\u8b58\u306f\u3001\u4e00\u81f4\u3059\u308b1+\u30012+\u30013+\u30a4\u30aa\u30f3\u30928\u30014\u30012.6667 m\/z\u30b7\u30d5\u30c8\u3055\u305b\u307e\u3059\u3002\"><figcaption>\u56f3 2\u3002\u4eee\u60f3\u7684\u306a 8 Da \u30e9\u30d9\u30eb\u306f\u3001\u96fb\u8377\u304c\u7570\u306a\u308b\u3068\u7570\u306a\u308b m\/z \u30b7\u30d5\u30c8\u3092\u4e0e\u3048\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>\u5fc5\u305a\u30da\u30a2\u540c\u58eb\u3092\u6bd4\u8f03\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044<\/h2>\n<p>\u540c\u3058\u96fb\u8377\u3068\u4ed8\u52a0\u7269\u30bf\u30a4\u30d7\u3092\u6301\u3064\u8efd\u30a4\u30aa\u30f3\u3068\u91cd\u30a4\u30aa\u30f3\u3092\u6bd4\u8f03\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u8efd\u30442\u4fa1\u30d4\u30fc\u30af\u3068\u91cd\u30443\u4fa1\u30d4\u30fc\u30af\u306f\u3001\u3053\u306e\u5358\u7d14\u306a\u5dee\u306e\u5f0f\u3067\u306f\u6bd4\u8f03\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n<p>\u540c\u4f4d\u4f53\u30d1\u30bf\u30fc\u30f3\u3068\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u60c5\u5831\u306f\u3001\u30da\u30a2\u306e\u78ba\u8a8d\u306b\u5f79\u7acb\u3061\u307e\u3059\u3002\u305d\u3046\u3067\u306a\u3051\u308c\u3070\u3001\u8fd1\u304f\u306b\u3042\u308b\u7121\u95a2\u4fc2\u306a\u30d4\u30fc\u30af\u3092\u91cd\u3044\u5f62\u614b\u3068\u8aa4\u8a8d\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u91cd\u3044\u30d4\u30fc\u30af\u304c\u3042\u308b\u304b\u3089\u3068\u3044\u3063\u3066\u3001\u81ea\u52d5\u7684\u306b\u5b9a\u91cf\u6a19\u6e96\u306b\u306a\u308b\u308f\u3051\u3067\u306f\u306a\u3044\u7406\u7531<\/h2>\n<p>\u5185\u90e8\u6a19\u6e96\u306f\u3001\u6e2c\u5b9a\u30b7\u30b0\u30ca\u30eb\u306e\u6bd4\u8f03\u3092\u52a9\u3051\u308b\u305f\u3081\u306b\u6dfb\u52a0\u3059\u308b\u7269\u8cea\u3067\u3059\u3002\u6a19\u8b58\u30da\u30d7\u30c1\u30c9\u3092\u305d\u306e\u3088\u3046\u306b\u4f7f\u3046\u306b\u306f\u3001\u305d\u306e\u540c\u4e00\u6027\u3068\u91cf\u3092\u628a\u63e1\u3057\u3001\u9078\u629e\u3057\u305f\u65b9\u6cd5\u3067\u306e\u6319\u52d5\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u6a19\u8b58\u306e\u5bcc\u5316\u7387\u3001\u6b8b\u5b58\u3059\u308b\u672a\u6a19\u8b58\u7269\u8cea\u3001\u8a66\u6599\u56de\u53ce\u7387\u304c\u5f71\u97ff\u3059\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u8efd\u3044\u30b7\u30b0\u30ca\u30eb\u3068\u91cd\u3044\u30b7\u30b0\u30ca\u30eb\u306e\u6bd4\u3092\u6fc3\u5ea6\u7d50\u679c\u306b\u3059\u308b\u306b\u306f\u3001\u9069\u5207\u306b\u6821\u6b63\u3055\u308c\u305f\u65b9\u6cd5\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t15\/en\/3.png\" alt=\"\u56f3 3\u3002\u5185\u90e8\u6a19\u6e96\u306b\u306f\u540c\u4e00\u6027\u3001\u91cf\u3001\u304a\u3088\u3073\u30e1\u30bd\u30c3\u30c9\u9069\u5408\u6027\u304c\u5fc5\u8981\u3067\u3042\u308a\u3001\u91cd\u3044\u30d4\u30fc\u30af\u3060\u3051\u3067\u306f\u4e0d\u5341\u5206\u3067\u3059\u3002\"><figcaption>\u56f3 3\u3002\u5b9a\u91cf\u5185\u90e8\u6a19\u6e96\u306b\u306f\u3001\u3088\u308a\u91cd\u3044\u8cea\u91cf\u30b7\u30b0\u30ca\u30eb\u3060\u3051\u3067\u306f\u4e0d\u5341\u5206\u3067\u3059\u3002<\/figcaption><\/figure>\n<h2>\u5225\u306e\u6a19\u8b58\u8cea\u91cf\u3092\u8a66\u3059<\/h2>\n<p>For a real order, state the complete sequence, labeled positions, isotope composition and end groups. \u201cHeavy peptide\u201d alone is not a complete specification.<\/p>\n<div class=\"exercise\"><strong>\u4eee\u8aac\u7684\u306a\u30e9\u30d9\u30eb\u306f10.0000 Da\u3092\u8ffd\u52a0\u3057\u307e\u3059\u3002\u4e00\u81f4\u3059\u308b\u4e8c\u91cd\u96fb\u8377\u30a4\u30aa\u30f3\u306f\u3069\u308c\u3060\u3051\u30b7\u30d5\u30c8\u3057\u307e\u3059\u304b\uff1f<\/strong><\/p>\n<details>\n<summary>\u7b54\u3048\u3092\u898b\u308b<\/summary>\n<p>10.0000 \u00f7 2 = 5.0000 m\/z.<\/p>\n<\/details>\n<\/div>\n<h2>\u6a19\u8b58MOTS-c\u914d\u5217\u306e\u30ea\u30af\u30a8\u30b9\u30c8<\/h2>\n<p>\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3067\u6a19\u8b58MOTS-c\u914d\u5217\u304c\u5fc5\u8981\u306a\u5834\u5408\u306f\u3001\u5b8c\u5168\u306a\u6a19\u8b58\u4ed5\u69d8\u3092\u30ab\u30b9\u30bf\u30e0\u30ea\u30af\u30a8\u30b9\u30c8\u3068\u3057\u3066\u63d0\u51fa\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u901a\u5e38\u306e\u30ab\u30bf\u30ed\u30b0\u8a18\u9332\u306f\u3001\u6a19\u8b58\u5909\u7570\u4f53\u304c\u5229\u7528\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3059\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/mots-c-human\/\">MOTS-c<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e5%bc%95%e7%94%a8\/\">\u8cc7\u6750\u304a\u3088\u3073\u6587\u66f8\u306e\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u3068\u76f8\u8ac7\u3059\u308b<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ja\/%e5%88%86%e6%9e%90%e6%89%8b%e6%b3%95\/lc-ms-%e5%90%8c%e4%b8%80%e6%80%a7%e6%a4%9c%e8%a8%bc\/\">\u5206\u6790\u30ac\u30a4\u30c9\u3092\u7d9a\u3051\u308b<\/a><\/p>\n<footer><strong>\u51fa\u5178\u3068\u4f8b\u306e\u6ce8\u8a18<\/strong><\/p>\n<p>\u6570\u5024\u3068\u66f2\u7dda\u306f\u6559\u80b2\u7528\u306e\u4f8b\u3067\u3042\u308a\u3001\u88fd\u54c1\u30d0\u30c3\u30c1\u306e\u8a66\u9a13\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u51fa\u5178\u78ba\u8a8d\u65e5\uff1a11 \u5e74 2026 \u6708\u3002<\/p>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\">Thermo Fisher Scientific \u00b7 mass spectrometry<\/a><\/li>\n<li><a href=\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\">ICH Q2(R2) \u00b7 analytical validation<\/a><\/li>\n<\/ul>\n<p>AI\u652f\u63f4\u306b\u3088\u308b\u57f7\u7b46\u304a\u3088\u3073\u60c5\u5831\u6e90\u306e\u30ec\u30d3\u30e5\u30fc\u3002\u30ab\u30bf\u30ed\u30b0\u8cc7\u6599\u306f\u7814\u7a76\u3001\u958b\u767a\u3001\u88fd\u9020\u7528\u3067\u3042\u308a\u3001\u4eba\u307e\u305f\u306f\u52d5\u7269\u3078\u306e\u4f7f\u7528\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u91cd\u6a19\u8b58\u306e\u8cea\u91cf\u5909\u5316\u3092\u96fb\u8377\u8a08\u7b97\u3092\u901a\u3057\u3066\u8ffd\u8de1\u3057\u307e\u3059\u3002<\/p>","protected":false},"author":1,"featured_media":3084,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"","pim_article_type":"Peptide buying and basics","pim_evidence_level":"Technical references and labeled calculations","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Examples are calculations, not NHD lot results. Confirm project feasibility and document availability.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"AI-assisted writing, source checks and illustrated cover.","pim_sources":"[{\"title\":\"Teaching reference \u2014 www.thermofisher.com \u2014 overview-mass-spectrometry.html\",\"note\":\"\",\"url\":\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\"},{\"title\":\"Teaching reference \u2014 database.ich.org \u2014 ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\",\"note\":\"\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3086","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>An 8 Da Heavy Label but a 4 m\/z Shift? 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