{"id":3443,"date":"2026-10-05T03:29:16","date_gmt":"2026-10-05T03:29:16","guid":{"rendered":"https:\/\/nhdbio.com\/?p=3443"},"modified":"2026-10-15T03:30:49","modified_gmt":"2026-10-15T03:30:49","slug":"%e5%87%8d%e7%b5%90%e4%b9%be%e7%87%a5%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e3%81%ae%e6%b0%b4%e5%88%86%e5%90%ab%e9%87%8f%e3%82%ab%e3%83%bc%e3%83%ab%e3%83%95%e3%82%a3%e3%83%83%e3%82%b7%e3%83%a3%e3%83%bc","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ja\/lyophilized-peptide-water-content-karl-fischer\/","title":{"rendered":"\u4e7e\u71e5\u3057\u3066\u898b\u3048\u308b\u30da\u30d7\u30c1\u30c9\u7c89\u672b\u3067\u3082\u6c34\u5206\u3092\u542b\u3080\u3053\u3068\u304c\u3042\u308a\u307e\u3059"},"content":{"rendered":"<p>Two vials contain dry-looking powder. Does that mean their water content is the same\u2014or zero? Appearance cannot tell you the percentage. A simple example shows why the distinction matters when you weigh a small sample.<\/p>\n<h2>\u51cd\u7d50\u4e7e\u71e5\u306f\u6c34\u5206\u30bc\u30ed\u3092\u6e2c\u5b9a\u3057\u305f\u3053\u3068\u306b\u306f\u306a\u3089\u306a\u3044<\/h2>\n<p>\u51cd\u7d50\u4e7e\u71e5\u306f\u6c34\u5206\u3092\u9664\u53bb\u3057\u307e\u3059\u304c\u3001\u6c34\u5206\u30bc\u30ed\u3068\u3044\u3046\u8a66\u9a13\u7d50\u679c\u3092\u78ba\u5b9a\u3059\u308b\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u4e7e\u71e5\u3057\u305f\u5916\u89b3\u306e\u30b1\u30fc\u30ad\u3084\u7c89\u672b\u3067\u3082\u6c34\u5206\u3092\u542b\u3080\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u5f62\u72b6\u306f\u89b3\u5bdf\u3067\u3042\u308a\u3001\u6c34\u5206\u6e2c\u5b9a\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p>\u6c34\u5206\u91cf\u304c\u91cd\u8981\u306a\u5834\u5408\u306f\u3001\u5199\u771f\u304b\u3089\u5224\u65ad\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u9069\u5207\u306a\u6e2c\u5b9a\u3092\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t18\/en\/1.png\" alt=\"\u56f3 1. \u4e7e\u3044\u305f\u5916\u89b3\u306e\u30b1\u30fc\u30ad\u306f\u5916\u89b3\u4e0a\u306e\u89b3\u5bdf\u3067\u3042\u308a\u3001\u6c34\u5206\u542b\u91cf\u306e\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\"><figcaption>\u56f3 1\u3002\u4e7e\u71e5\u3057\u305f\u5916\u89b3\u3068\u6e2c\u5b9a\u3055\u308c\u305f\u6c34\u5206\u542b\u91cf\u306f\u7570\u306a\u308b\u89b3\u5bdf\u3067\u3059\u3002<\/figcaption><\/figure>\n<h2>10 mg\u306b\u304a\u3051\u308b3%\u6c34\u5206\u3068\u306f\u4f55\u3092\u610f\u5473\u3057\u307e\u3059\u304b\u3002<\/h2>\n<p>Assume a suitable test finds 3.0% water by weight in the sample as received. In 10 mg of powder, water weighs 10 \u00d7 0.03 = 0.3 mg. The non-water portion is 9.7 mg.<\/p>\n<p>\u6b8b\u308a\u306e9.7 mg\u304c\u81ea\u52d5\u7684\u306b\u3059\u3079\u3066\u76ee\u7684\u30da\u30d7\u30c1\u30c9\u3067\u3042\u308b\u3068\u306f\u9650\u308a\u307e\u305b\u3093\u3002\u5869\u6210\u5206\u3084\u305d\u306e\u4ed6\u306e\u7269\u8cea\u3092\u542b\u3080\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t18\/en\/2.png\" alt=\"\u56f3 2. \u67b6\u7a7a\u306e 10 mg \u7c89\u672b\u306b\u304a\u3044\u3066\u3001\u8a18\u9332\u3055\u308c\u305f 3% \u306e\u6c34\u5206\u7387\u306f 0.3 mg \u306e\u6c34\u306b\u76f8\u5f53\u3057\u307e\u3059\u3002\"><figcaption>\u56f3 2\u3002\u3053\u306e\u4f8b\u3067\u306f\u30013% \u306e\u6c34\u306b\u542b\u307e\u308c\u308b 10 mg \u306f 0.3 mg \u306e\u6c34\u3092\u542b\u3093\u3067\u3044\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>\u6c34\u5206\u8a66\u9a13\u306f\u4f55\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u304b\uff1f<\/h2>\n<p>\u30ab\u30fc\u30eb\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u6cd5\u306f\u3001\u6c34\u306e\u6e2c\u5b9a\u306b\u9069\u3057\u305f\u5316\u5b66\u53cd\u5fdc\u3092\u5229\u7528\u3057\u307e\u3059\u3002\u8a66\u6599\u8abf\u88fd\u3068\u624b\u9806\u306f\u3001\u6750\u6599\u306b\u9069\u3057\u305f\u3082\u306e\u3067\u3042\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u7d50\u679c\u3068\u5408\u308f\u305b\u3066\u305d\u308c\u3089\u306e\u8a73\u7d30\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u4e7e\u71e5\u6e1b\u91cf\u306f\u3001\u6307\u5b9a\u3055\u308c\u305f\u6761\u4ef6\u4e0b\u3067\u5931\u308f\u308c\u305f\u91cd\u91cf\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u4ed6\u306e\u63ee\u767a\u6027\u7269\u8cea\u3082\u305d\u306e\u6e1b\u91cf\u306b\u5bc4\u4e0e\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001\u81ea\u52d5\u7684\u306b\u6c34\u5206\u7279\u7570\u7684\u306a\u7d50\u679c\u3068\u540c\u3058\u306b\u306a\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<h2>\u306a\u305c\u4e7e\u71e5\u57fa\u6e96\u306e\u5272\u5408\u306f\u9ad8\u304f\u898b\u3048\u308b\u306e\u304b<\/h2>\n<p>Suppose the 10 mg sample contains 8 mg target peptide and 0.3 mg water. On the original sample weight, peptide is 8 \u00f7 10 = 80%. If only the water is removed from the denominator, it is 8 \u00f7 9.7 \u00d7 100% \u2248 82.47%.<\/p>\n<p>\u30da\u30d7\u30c1\u30c9\u91cf\u306f\u5897\u52a0\u3057\u3066\u3044\u307e\u305b\u3093\u3002\u5206\u6bcd\u3068\u3057\u3066\u4f7f\u7528\u3055\u308c\u308b\u91cd\u91cf\u304c\u6e1b\u5c11\u3057\u305f\u306e\u3067\u3059\u3002\u53d7\u3051\u5165\u308c\u6642\u306e\u79e4\u91cf\u8a08\u7b97\u306b\u4e7e\u71e5\u57fa\u6e96\u306e\u7d50\u679c\u3092\u76f4\u63a5\u4f7f\u7528\u3057\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t18\/en\/3.png\" alt=\"\u56f3 3. \u305d\u306e\u307e\u307e\u57fa\u6e96\u3068\u4e7e\u71e5\u57fa\u6e96\u306e\u7d50\u679c\u3067\u306f\u5206\u6bcd\u304c\u7570\u306a\u308a\u307e\u3059\u3002\"><figcaption>\u56f3 3\u3002\u5206\u6bcd\u3092\u5909\u3048\u308b\u3068\u5272\u5408\u306f\u5909\u308f\u308a\u307e\u3059\u304c\u3001\u30da\u30d7\u30c1\u30c9\u91cf\u306f\u5909\u308f\u308a\u307e\u305b\u3093\u3002<\/figcaption><\/figure>\n<h2>\u6c34\u5206\u542b\u91cf\u306f\u4fdd\u5b58\u5bff\u547d\u306e\u8a66\u9a13\u3067\u306f\u306a\u3044<\/h2>\n<p>\u3044\u3044\u3048\u3002\u5b89\u5b9a\u6027\u306f\u6750\u6599\u3001\u5305\u88c5\u3001\u53d6\u6271\u3044\u3001\u304a\u3088\u3073\u88cf\u4ed8\u3051\u3068\u306a\u308b\u8a66\u9a13\u306b\u3088\u3063\u3066\u6c7a\u307e\u308a\u307e\u3059\u3002\u6c34\u5206\u542b\u91cf\u3001HPLC\u7d14\u5ea6\u3001\u76ee\u7684\u30da\u30d7\u30c1\u30c9\u91cf\u306f\u305d\u308c\u305e\u308c\u5225\u306e\u7d50\u679c\u3067\u3059\u3002<\/p>\n<div class=\"exercise\"><strong>5 mg\u8a66\u6599\u306b\u306f\u3001\u91cd\u91cf\u6bd4\u30674%\u306e\u6c34\u5206\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u6c34\u5206\u91cf\u306f\u3069\u308c\u3060\u3051\u3067\u3059\u304b\uff1f<\/strong><\/p>\n<details>\n<summary>\u7b54\u3048\u3092\u898b\u308b<\/summary>\n<p>5 \u00d7 0.04 = 0.2 mg water. The other 4.8 mg is non-water material, not necessarily all target peptide.<\/p>\n<\/details>\n<\/div>\n<h2>MOTS-c\u307e\u305f\u306fKPV\u306e\u91cf\u3092\u6bd4\u8f03\u3059\u308b<\/h2>\n<p>MOTS-c\u307e\u305f\u306fKPV\u306e\u91cf\u3092\u6bd4\u8f03\u3059\u308b\u5834\u5408\u306f\u3001\u30e9\u30d9\u30eb\u91cd\u91cf\u304c\u4f55\u3092\u8868\u3059\u304b\u3001\u304a\u3088\u3073\u95a2\u9023\u3059\u308b\u6c34\u5206\u307e\u305f\u306f\u30da\u30d7\u30c1\u30c9\u542b\u91cf\u306e\u7d50\u679c\u304c\u5b58\u5728\u3059\u308b\u304b\u3092\u660e\u78ba\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5916\u89b3\u304b\u3089\u6c34\u5206\u898f\u683c\u3092\u78ba\u5b9a\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/mots-c-human\/\">MOTS-c<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/kpv\/\">KPV<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e5%bc%95%e7%94%a8\/\">\u8cc7\u6750\u304a\u3088\u3073\u6587\u66f8\u306e\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u3068\u76f8\u8ac7\u3059\u308b<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ja\/%e5%88%86%e6%9e%90%e6%89%8b%e6%b3%95\/%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e5%90%ab%e6%9c%89%e9%87%8f%e3%81%a8%e7%b4%94%e5%ba%a6%e3%81%ae%e9%96%a2%e4%bf%82\/\">\u5206\u6790\u30ac\u30a4\u30c9\u3092\u7d9a\u3051\u308b<\/a><\/p>\n<footer><strong>\u51fa\u5178\u3068\u4f8b\u306e\u6ce8\u8a18<\/strong><\/p>\n<p>\u6570\u5024\u3068\u66f2\u7dda\u306f\u6559\u80b2\u7528\u306e\u4f8b\u3067\u3042\u308a\u3001\u88fd\u54c1\u30d0\u30c3\u30c1\u306e\u8a66\u9a13\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u51fa\u5178\u78ba\u8a8d\u65e5\uff1a11 \u5e74 2026 \u6708\u3002<\/p>\n<ul>\n<li><a href=\"https:\/\/www.bachem.com\/knowledge-center\/quality-control-of-amino-acids-peptides-a-guide\/\">Bachem \u00b7 peptide quality control<\/a><\/li>\n<li><a href=\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\">ICH Q2(R2) \u00b7 analytical validation<\/a><\/li>\n<\/ul>\n<p>AI\u652f\u63f4\u306b\u3088\u308b\u57f7\u7b46\u304a\u3088\u3073\u60c5\u5831\u6e90\u306e\u30ec\u30d3\u30e5\u30fc\u3002\u30ab\u30bf\u30ed\u30b0\u8cc7\u6599\u306f\u7814\u7a76\u3001\u958b\u767a\u3001\u88fd\u9020\u7528\u3067\u3042\u308a\u3001\u4eba\u307e\u305f\u306f\u52d5\u7269\u3078\u306e\u4f7f\u7528\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u6c34\u5206\u542b\u91cf\u3068\u30da\u30d7\u30c1\u30c9\u542b\u91cf\u3092\u5206\u3051\u308b\u305f\u3081\u306b\u3001\u5c0f\u3055\u306a\u91cd\u91cf\u306e\u4f8b\u3092\u4f7f\u3044\u307e\u3059\u3002<\/p>","protected":false},"author":1,"featured_media":3961,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"329,344,318,369","pim_article_type":"Practical research guide","pim_evidence_level":"Technical guidance with labeled teaching examples","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Study results and labeled examples are separated. Original-source, arithmetic, readability and internal-link checks; no independent human peer review recorded.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"AI-assisted writing and source checks; conceptual illustrated cover.","pim_sources":"[{\"title\":\"Bachem: Quality Control of Amino Acids and Peptides\",\"note\":\"Bachem: Quality Control of Amino Acids and Peptides. Accessed 2026-10-05.\",\"url\":\"https:\/\/www.bachem.com\/knowledge-center\/quality-control-of-amino-acids-peptides-a-guide\/\"},{\"title\":\"Teaching reference \u2014 database.ich.org \u2014 ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\",\"note\":\"\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\"},{\"title\":\"Care and handling of peptides\",\"note\":\"Care and handling of peptides\",\"url\":\"https:\/\/www.bachem.com\/knowledge-center\/care-and-handling-of-peptides\/\"}]","footnotes":""},"categories":[20],"tags":[],"class_list":["post-3443","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evidence-quality-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Dry-Looking Peptide Powder Can Still Contain Water | NHD<\/title>\n<meta name=\"description\" content=\"Use a small weight example to separate water content from peptide content.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nhdbio.com\/ja\/\u51cd\u7d50\u4e7e\u71e5\u30da\u30d7\u30c1\u30c9\u306e\u6c34\u5206\u542b\u91cf\u30ab\u30fc\u30eb\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Dry-Looking Peptide Powder Can Still Contain Water\" \/>\n<meta property=\"og:description\" content=\"Use a small weight example to separate water content from peptide content.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/nhdbio.com\/ja\/\u51cd\u7d50\u4e7e\u71e5\u30da\u30d7\u30c1\u30c9\u306e\u6c34\u5206\u542b\u91cf\u30ab\u30fc\u30eb\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\/\" \/>\n<meta property=\"og:site_name\" content=\"NHD\" \/>\n<meta property=\"article:published_time\" content=\"2026-10-05T03:29:16+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-10-15T03:30:49+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/nhd-comic-3443.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"800\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"NHD Technical Team\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:description\" content=\"Peptide Raw Materials and Custom Peptide Synthesis\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"NHD Technical Team\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"2\u5206\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/lyophilized-peptide-water-content-karl-fischer\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/lyophilized-peptide-water-content-karl-fischer\\\/\"},\"author\":{\"name\":\"NHD Technical Team\",\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#\\\/schema\\\/person\\\/0ffb0dec7fda0f720073fc468fac6fc1\"},\"headline\":\"Dry-Looking Peptide Powder Can Still Contain Water\",\"datePublished\":\"2026-10-05T03:29:16+00:00\",\"dateModified\":\"2026-10-15T03:30:49+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/lyophilized-peptide-water-content-karl-fischer\\\/\"},\"wordCount\":441,\"publisher\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/nhdbio.com\\\/lyophilized-peptide-water-content-karl-fischer\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/nhdbio.com\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/nhd-comic-3443.jpg\",\"articleSection\":[\"Evidence &amp; 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