{"id":543,"date":"2026-08-27T18:00:00","date_gmt":"2026-08-27T18:00:00","guid":{"rendered":"https:\/\/nhdbio.com\/?p=543"},"modified":"2026-10-11T07:39:44","modified_gmt":"2026-10-11T07:39:44","slug":"lc%e3%80%81ms%e3%80%81%e3%83%9a%e3%83%97%e3%83%81%e3%83%89%e5%90%8c%e4%b8%80%e6%80%a7%e3%80%81%e6%ad%a3%e7%a2%ba%e8%b3%aa%e9%87%8f%e3%80%81%e5%90%8c%e4%bd%8d%e4%bd%93%e3%80%81msms","status":"publish","type":"post","link":"https:\/\/nhdbio.com\/ja\/lc-ms-peptide-identity-exact-mass-isotopes-msms\/","title":{"rendered":"\u8cea\u91cf\u304c\u4e00\u81f4\u3057\u3066\u3082\u914d\u5217\u5168\u4f53\u3067\u306f\u306a\u3044\uff1a\u30da\u30d7\u30c1\u30c9MS\/MS\u5165\u9580"},"content":{"rendered":"<p>The words \u201cact\u201d and \u201ccat\u201d use the same letters but mean different things. Peptide sequences can also use the same building blocks in a different order. Their complete masses may match even though their sequences do not.<\/p>\n<h2>3\u3064\u306e\u6587\u5b57\u304b\u3089\u59cb\u3081\u307e\u3057\u3087\u3046<\/h2>\n<p>A\u3001G\u3001K\u306f\u305d\u308c\u305e\u308c\u30a2\u30e9\u30cb\u30f3\u3001\u30b0\u30ea\u30b7\u30f3\u3001\u30ea\u30b8\u30f3\u306e\u7565\u53f7\u3067\u3059\u3002AGK\u3068GAK\u306f\u305d\u308c\u305e\u308c1\u3064\u305a\u3064\u542b\u3093\u3067\u3044\u307e\u3059\u3002\u672b\u7aef\u304c\u4e00\u81f4\u3057\u3001\u4ed6\u306e\u4fee\u98fe\u304c\u306a\u3051\u308c\u3070\u3001\u5168\u4f53\u306e\u7d44\u6210\u3068\u5b8c\u5168\u306a\u8cea\u91cf\u306f\u4e00\u81f4\u3057\u307e\u3059\u3002<\/p>\n<p>\u7570\u306a\u308b\u306e\u306f\u9806\u5e8f\u3067\u3059\u3002\u7dcf\u8cea\u91cf\u306e\u6e2c\u5b9a\u3067\u306f\u3001\u540c\u3058\u69cb\u6210\u8981\u7d20\u306e\u3059\u3079\u3066\u306e\u914d\u5217\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t12\/en\/1.png\" alt=\"\u56f31\u3002\u4f8b\u793a\u914d\u5217AGK\u3068GAK\u306f\u3001\u540c\u3058\u6b8b\u57fa\u3092\u7570\u306a\u308b\u9806\u5e8f\u3067\u542b\u3093\u3067\u3044\u307e\u3059\u3002\"><figcaption>\u56f31\u3002AGK\u3068GAK\u306f\u540c\u3058\u69cb\u6210\u8981\u7d20\u3092\u7570\u306a\u308b\u9806\u5e8f\u3067\u542b\u3093\u3067\u3044\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>MS\/MS\u306f\u3069\u306e\u3088\u3046\u306a\u8ffd\u52a0\u306e\u30b9\u30c6\u30c3\u30d7\u3092\u52a0\u3048\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/h2>\n<p>MS\/MS\u306f\u307e\u305a\u30da\u30d7\u30c1\u30c9\u304b\u3089\u30a4\u30aa\u30f3\u30921\u3064\u9078\u629e\u3057\u3001\u6b21\u306b\u305d\u308c\u3092\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306b\u5206\u89e3\u3057\u3066\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u9396\u5168\u4f53\u3060\u3051\u3092\u8a08\u91cf\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u9396\u306e\u65ad\u7247\u306b\u95a2\u3059\u308b\u60c5\u5831\u3082\u53ce\u96c6\u3057\u307e\u3059\u3002<\/p>\n<p>\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306f\u5206\u5b50\u3068\u8a66\u9a13\u6761\u4ef6\u306b\u4f9d\u5b58\u3057\u307e\u3059\u3002\u6559\u80b2\u7528\u306e\u306f\u3057\u3054\u306f\u8003\u3048\u65b9\u3092\u793a\u3059\u3082\u306e\u3067\u3042\u308a\u3001\u8003\u3048\u5f97\u308b\u3059\u3079\u3066\u306e\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u304c\u5b9f\u969b\u306e\u30b9\u30da\u30af\u30c8\u30eb\u306b\u73fe\u308c\u308b\u3053\u3068\u3092\u7d04\u675f\u3059\u308b\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<h2>\u7c21\u5358\u306a\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u30e9\u30c0\u30fc\u3092\u8aad\u3080<\/h2>\n<p>\u7c21\u7565\u5316\u3057\u305fb\u30a4\u30aa\u30f3\u30e9\u30c0\u30fc\u306f\u3001N\u672b\u7aef\u5074\u304b\u3089\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u3092\u305f\u3069\u308a\u307e\u3059\u3002AGK\u306e\u4f8b\u3067\u306f\u3001\u6700\u521d\u306e\u30d4\u30fc\u30b9\u306fA\u3068AG\u306b\u5bfe\u5fdc\u3057\u307e\u3059\u3002GAK\u3067\u306f\u3001G\u3068GA\u306b\u5bfe\u5fdc\u3057\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u6700\u521d\u306e\u30d4\u30fc\u30b9\u306f\u914d\u5217\u3092\u898b\u5206\u3051\u308b\u52a9\u3051\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u5b9f\u969b\u306e\u5e30\u5c5e\u306b\u306f\u3001\u6b63\u3057\u3044\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306e\u5316\u5b66\u3001\u96fb\u8377\u3001\u6e2c\u5b9a\u8cea\u91cf\u304c\u5fc5\u8981\u3067\u3059\u3002\u56f3\u306f\u914d\u5217\u306e\u90e8\u5206\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u6e2c\u5b9a\u3055\u308c\u305f\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u8cea\u91cf\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t12\/en\/2.png\" alt=\"\u56f3 2\u3002\u6982\u5ff5\u7684b\u30a4\u30aa\u30f3\u30e9\u30c0\u30fc\u304cN\u672b\u7aef\u304b\u3089\u6210\u9577\u3057\u307e\u3059\u3002\u6e2c\u5b9a\u3055\u308c\u305f\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u8cea\u91cf\u306f\u793a\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3002\"><figcaption>\u56f3 2\u3002\u6982\u5ff5\u7684\u306ab\u30a4\u30aa\u30f3\u30e9\u30c0\u30fc\u306f\u3001\u6700\u521d\u306e\u914d\u5217\u4f4d\u7f6e\u3092\u533a\u5225\u3067\u304d\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>\u4e00\u90e8\u306e\u533a\u5225\u306b\u306f\u4ed6\u306e\u65b9\u6cd5\u304c\u4f9d\u7136\u3068\u3057\u3066\u5fc5\u8981\u3067\u3059<\/h2>\n<p>\u30ed\u30a4\u30b7\u30f3\u3068\u30a4\u30bd\u30ed\u30a4\u30b7\u30f3\u306f\u540c\u3058\u5143\u7d20\u7d44\u6210\u3092\u6301\u3061\u307e\u3059\u3002\u57fa\u672c\u7684\u306a\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u8cea\u91cf\u30e9\u30c0\u30fc\u3067\u306f\u3001\u305d\u308c\u3089\u3092\u533a\u5225\u3067\u304d\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u901a\u5e38\u306e\u8cea\u91cf\u30de\u30c3\u30c1\u30f3\u30b0\u3082\u3001D\u5bfeL\u306e\u7acb\u4f53\u5316\u5b66\u3092\u78ba\u5b9a\u3057\u307e\u305b\u3093\u3002<\/p>\n<p>\u30c7\u30fc\u30bf\u304c\u3069\u306e\u4f4d\u7f6e\u3092\u652f\u6301\u3057\u3001\u3069\u306e\u4f4d\u7f6e\u304c\u66d6\u6627\u306a\u307e\u307e\u304b\u3092\u5c0b\u306d\u3066\u304f\u3060\u3055\u3044\u3002\u5831\u544a\u66f8\u306f\u3001\u3059\u3079\u3066\u306e\u8cea\u91cf\u30de\u30c3\u30c1\u3092\u5b8c\u5168\u306a\u914d\u5217\u78ba\u8a8d\u3068\u3057\u3066\u6271\u3046\u306e\u3067\u306f\u306a\u304f\u3001\u305d\u306e\u3053\u3068\u3092\u8aac\u660e\u3059\u3079\u304d\u3067\u3059\u3002<\/p>\n<figure><img decoding=\"async\" src=\"https:\/\/nhdbio.com\/wp-content\/plugins\/nhd-content-refresh\/teaching-images\/t12\/en\/3.png\" alt=\"\u56f3 3\u3002\u30a4\u30f3\u30bf\u30af\u30c8\u8cea\u91cf\u3068\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306e\u8a3c\u62e0\u306f\u3001\u7570\u306a\u308b\u540c\u4e00\u6027\u306e\u7591\u554f\u306b\u7b54\u3048\u307e\u3059\u3002\"><figcaption>\u56f3 3\u3002\u5206\u5b50\u5168\u4f53\u306e\u8cea\u91cf\u3068\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u30c7\u30fc\u30bf\u306f\u3001\u7570\u306a\u308b\u554f\u3044\u306b\u7b54\u3048\u307e\u3059\u3002<\/figcaption><\/figure>\n<h2>\u8cea\u91cf\u4e00\u81f4\u306e\u78ba\u8a8d\u3068\u914d\u5217\u4e00\u81f4\u306e\u78ba\u8a8d\u3092\u5206\u3051\u3066\u8003\u3048\u308b<\/h2>\n<p>\u30a4\u30f3\u30bf\u30af\u30c8\u8cea\u91cf\u3082MS\/MS\u3082\u3001\u305d\u308c\u3060\u3051\u3067\u306fHPLC\u7d14\u5ea6\u3084\u751f\u7269\u5b66\u7684\u6d3b\u6027\u306e\u7d50\u679c\u3092\u81ea\u52d5\u7684\u306b\u793a\u3059\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u5404\u6e2c\u5b9a\u306f\u3001\u5b9f\u969b\u306b\u4f55\u3092\u8abf\u3079\u3066\u3044\u308b\u306e\u304b\u306b\u5fdc\u3058\u3066\u8aac\u660e\u3055\u308c\u308b\u3079\u304d\u3067\u3059\u3002<\/p>\n<div class=\"exercise\"><strong>AGK\u3068GAK\u306f\u5b8c\u5168\u306a\u8cea\u91cf\u304c\u540c\u3058\u3067\u3059\u3002\u305d\u308c\u306f\u540c\u3058\u914d\u5217\u3067\u3042\u308b\u3053\u3068\u3092\u8a3c\u660e\u3057\u307e\u3059\u304b\uff1f<\/strong><\/p>\n<details>\n<summary>\u7b54\u3048\u3092\u898b\u308b<\/summary>\n<p>\u3044\u3044\u3048\u3002\u6700\u521d\u306e2\u3064\u306e\u4f4d\u7f6e\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u9069\u5207\u306a\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u8a3c\u62e0\u304c\u3042\u308c\u3070\u3001\u305d\u308c\u3089\u3092\u533a\u5225\u3059\u308b\u52a9\u3051\u306b\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<\/details>\n<\/div>\n<h2>MOTS-c\u307e\u305f\u306fLL-37\u306e\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3067\u914d\u5217\u60c5\u5831\u304c\u5fc5\u8981\u306a\u5834\u5408<\/h2>\n<p>MOTS-c\u307e\u305f\u306fLL-37\u306e\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3067\u914d\u5217\u30ec\u30d9\u30eb\u60c5\u5831\u304c\u5fc5\u8981\u306a\u5834\u5408\u306f\u3001\u9069\u5207\u306aMS\/MS\u30c7\u30fc\u30bf\u304c\u5229\u7528\u53ef\u80fd\u304b\u3069\u3046\u304b\u3092\u5177\u4f53\u7684\u306b\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30a4\u30f3\u30bf\u30af\u30c8\u8cea\u91cf\u3092\u5831\u544a\u3057\u3066\u3044\u308b\u6587\u66f8\u306b\u306f\u3001\u3053\u306e\u8ffd\u52a0\u89e3\u6790\u304c\u542b\u307e\u308c\u3066\u3044\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/mots-c-human\/\">MOTS-c<\/a> \/ <a href=\"https:\/\/nhdbio.com\/ja\/%e8%a3%bd%e5%93%81\/ll-37\/\">LL-37<\/a><\/p>\n<p><a href=\"https:\/\/nhdbio.com\/ja\/%e5%bc%95%e7%94%a8\/\">\u8cc7\u6750\u304a\u3088\u3073\u6587\u66f8\u306e\u8981\u4ef6\u306b\u3064\u3044\u3066NHD\u3068\u76f8\u8ac7\u3059\u308b<\/a> \u00b7 <a href=\"https:\/\/nhdbio.com\/ja\/%e5%88%86%e6%9e%90%e6%89%8b%e6%b3%95\/lc-ms-%e5%90%8c%e4%b8%80%e6%80%a7%e6%a4%9c%e8%a8%bc\/\">\u5206\u6790\u30ac\u30a4\u30c9\u3092\u7d9a\u3051\u308b<\/a><\/p>\n<footer><strong>\u51fa\u5178\u3068\u4f8b\u306e\u6ce8\u8a18<\/strong><\/p>\n<p>\u6570\u5024\u3068\u66f2\u7dda\u306f\u6559\u80b2\u7528\u306e\u4f8b\u3067\u3042\u308a\u3001\u88fd\u54c1\u30d0\u30c3\u30c1\u306e\u8a66\u9a13\u7d50\u679c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u51fa\u5178\u78ba\u8a8d\u65e5\uff1a11 \u5e74 2026 \u6708\u3002<\/p>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\">Thermo Fisher Scientific \u00b7 mass spectrometry<\/a><\/li>\n<li><a href=\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\">ICH Q2(R2) \u00b7 analytical validation<\/a><\/li>\n<\/ul>\n<p>AI\u652f\u63f4\u306b\u3088\u308b\u57f7\u7b46\u304a\u3088\u3073\u60c5\u5831\u6e90\u306e\u30ec\u30d3\u30e5\u30fc\u3002\u30ab\u30bf\u30ed\u30b0\u8cc7\u6599\u306f\u7814\u7a76\u3001\u958b\u767a\u3001\u88fd\u9020\u7528\u3067\u3042\u308a\u3001\u4eba\u307e\u305f\u306f\u52d5\u7269\u3078\u306e\u4f7f\u7528\u3092\u76ee\u7684\u3068\u3057\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n<\/footer>","protected":false},"excerpt":{"rendered":"<p>\u77ed\u3044\u914d\u5217\u30922\u3064\u4f7f\u3063\u3066\u3001\u8cea\u91cf\u304c\u4e00\u81f4\u3057\u3066\u3082\u9806\u5e8f\u304c\u4e00\u81f4\u3059\u308b\u8a3c\u62e0\u306b\u306f\u306a\u3089\u306a\u3044\u7406\u7531\u3092\u78ba\u304b\u3081\u307e\u3057\u3087\u3046\u3002<\/p>","protected":false},"author":1,"featured_media":4026,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"pim_family_ids":"","pim_article_type":"Quality documentation guide","pim_evidence_level":"ICH Q6B\/FDA peptide-characterization principles plus peer-reviewed LC-MS methods; no lot-specific spectrum","pim_reviewer":"AI-assisted source and editorial check","pim_reviewed_date":"2026-10-11","pim_editorial_note":"Illustrated spectra are editorial abstractions. This guide does not authenticate a sequence or catalog lot; interpretation requires the original method, raw data and sample traceability.","pim_review_status":"reviewed","pim_review_reason":"Teaching examples, arithmetic, source links and plain language checked; product enquiries linked.","pim_creation_method":"The editorial workflow starts with linked regulatory guidance and peer-reviewed analytical-method records; extracts scope, definitions, required fields and limitations; and separates general method principles from product- and lot-specific claims. AI-assisted drafting was used to structure the first version. Claims, examples and boundaries were checked against the linked records in the site editorial workflow. This is not independent peer review or evidence that any catalog lot has passed the tests described.","pim_sources":"[{\"title\":\"ICH Q6B: Specifications\u2014Test Procedures and Acceptance Criteria for Biotechnological\/Biological Products\",\"note\":\"Discusses mass spectrometry and peptide mapping as characterization tools.\",\"url\":\"https:\/\/www.fda.gov\/media\/71510\/download\"},{\"title\":\"FDA: ANDAs for Certain Highly Purified Synthetic Peptide Drug Products\",\"note\":\"Discusses peptide-related impurities and why robust analytical characterization matters.\",\"url\":\"https:\/\/www.fda.gov\/media\/107622\/download\"},{\"title\":\"High-resolution MS\/MS for synthetic peptide sequence confirmation\",\"note\":\"Peer-reviewed method record; PMID 26424266.\",\"url\":\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26424266\/\"},{\"title\":\"LC-MS\/MS characterization of synthetic peptide impurities\",\"note\":\"Peer-reviewed impurity-characterization record; PMID 19953542.\",\"url\":\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19953542\/\"},{\"title\":\"Teaching reference \u2014 www.thermofisher.com \u2014 overview-mass-spectrometry.html\",\"note\":\"\",\"url\":\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/overview-mass-spectrometry.html\"},{\"title\":\"Teaching reference \u2014 database.ich.org \u2014 ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\",\"note\":\"\",\"url\":\"https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2(R2)_Guideline_2023_1130_ErrorCorrection_2025.pdf\"}]","footnotes":""},"categories":[21],"tags":[],"class_list":["post-543","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-quality-documentation-guides"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.0 (Yoast SEO v28.1) - 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