{"version":"1.0","provider_name":"NHD","provider_url":"https:\/\/nhdbio.com\/pt-br","author_name":"NHD Technical Team","author_url":"https:\/\/nhdbio.com\/pt-br\/author\/qianmiao-technical-team\/","title":"TFA or formic acid: why does the same peptide give different results?","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"hkVIubnlE8\"><a href=\"https:\/\/nhdbio.com\/pt-br\/analytical-methods\/tfa-formic-acid-peptide-lc-ms\/\">TFA ou \u00e1cido f\u00f3rmico: por que o mesmo pept\u00eddeo d\u00e1 resultados diferentes?<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/nhdbio.com\/pt-br\/analytical-methods\/tfa-formic-acid-peptide-lc-ms\/embed\/#?secret=hkVIubnlE8\" width=\"600\" height=\"338\" title=\"&#8220;TFA or formic acid: why does the same peptide give different results?&#8221; &#8212; NHD\" data-secret=\"hkVIubnlE8\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/nhdbio.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>","thumbnail_url":"https:\/\/nhdbio.com\/wp-content\/uploads\/2026\/10\/nhd-knowledge-20261013-07.webp","thumbnail_width":1672,"thumbnail_height":941,"description":"TFA and formic acid can both serve as mobile phase additives in some peptide analysis methods, but they affect separation and mass spectrometry response differently. When you change the additive, you cannot just look at which number is larger."}